Portable in situ fluorescence cytometry of microscale cell-based assays

Daniel A. Tatosian, Michael L. Shuler, Donghyun Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A portable fluorescence cytometric system has been developed for characterizing chemical concentration and cellular status in microscale cell culture analog (μCCA) devices. Based on discrete optical components, the system provides a modular platform for real-time image measurements applicable to a variety of cell-based microassays. As a feasibility study, we investigated the real-time dynamics of daunorubicin uptake with cultured mouse L-cells in a μCCA compartment. Time course results measured by the portable fluorescence cytometric system confirmed that in the μCCA devices daunorubicin accumulation is proportional to the liquid turnover rate.

Original languageEnglish
Pages (from-to)1689-1691
Number of pages3
JournalOptics Letters
Volume30
Issue number13
DOIs
Publication statusPublished - 2005 Jul 1

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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