TY - GEN
T1 - Portable real-time fluorescence cytometry of microscale cell culture analog devices
AU - Kim, Donghyun
AU - Tatosian, Daniel A.
AU - Shuler, Michael L.
PY - 2006/5/8
Y1 - 2006/5/8
N2 - A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.
AB - A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.
UR - http://www.scopus.com/inward/record.url?scp=33646202794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646202794&partnerID=8YFLogxK
U2 - 10.1117/12.644521
DO - 10.1117/12.644521
M3 - Conference contribution
AN - SCOPUS:33646202794
SN - 081946130X
SN - 9780819461308
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
Y2 - 23 January 2006 through 25 January 2006
ER -