Abstract
A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.
| Original language | English |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 6088 |
| DOIs | |
| Publication status | Published - 2006 May 8 |
| Event | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV - San Jose, CA, United States Duration: 2006 Jan 23 → 2006 Jan 25 |
Other
| Other | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 06/1/23 → 06/1/25 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging
Cite this
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Portable real-time fluorescence cytometry of microscale cell culture analog devices. / Kim, Donghyun; Tatosian, Daniel A.; Shuler, Michael L.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6088 2006. 60880X.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Portable real-time fluorescence cytometry of microscale cell culture analog devices
AU - Kim, Donghyun
AU - Tatosian, Daniel A.
AU - Shuler, Michael L.
PY - 2006/5/8
Y1 - 2006/5/8
N2 - A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.
AB - A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.
UR - http://www.scopus.com/inward/record.url?scp=33646202794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646202794&partnerID=8YFLogxK
U2 - 10.1117/12.644521
DO - 10.1117/12.644521
M3 - Conference contribution
SN - 081946130X
SN - 9780819461308
VL - 6088
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -