Abstract
A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.
Original language | English |
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Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
DOIs | |
Publication status | Published - 2006 |
Event | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV - San Jose, CA, United States Duration: 2006 Jan 23 → 2006 Jan 25 |
Publication series
Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
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Volume | 6088 |
ISSN (Print) | 1605-7422 |
Other
Other | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV |
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Country/Territory | United States |
City | San Jose, CA |
Period | 06/1/23 → 06/1/25 |
Bibliographical note
Funding Information:This study was supported by a grant from the National R&D Program for Cancer Control, Ministry of Health and Welfare, Republic of Korea (HA16C0015) (1720150).
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomaterials
- Radiology Nuclear Medicine and imaging