Portable real-time fluorescence cytometry of microscale cell culture analog devices

Donghyun Kim; Daniel A. Tatosian; Michael L. Shuler

doi:10.1117/12.644521

Portable real-time fluorescence cytometry of microscale cell culture analog devices

Donghyun Kim, Daniel A. Tatosian, Michael L. Shuler

Department of Electrical and Electronic Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.

Original language	English
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
DOIs	https://doi.org/10.1117/12.644521
Publication status	Published - 2006
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV - San Jose, CA, United States Duration: 2006 Jan 23 → 2006 Jan 25

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6088
ISSN (Print)	1605-7422

Other

Other	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
Country	United States
City	San Jose, CA
Period	06/1/23 → 06/1/25

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

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Cite this

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title = "Portable real-time fluorescence cytometry of microscale cell culture analog devices",

abstract = "A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.",

author = "Donghyun Kim and Tatosian, {Daniel A.} and Shuler, {Michael L.}",

year = "2006",

doi = "10.1117/12.644521",

language = "English",

isbn = "081946130X",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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Kim, D, Tatosian, DA & Shuler, ML 2006, Portable real-time fluorescence cytometry of microscale cell culture analog devices. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE., 60880X, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, San Jose, CA, United States, 06/1/23. https://doi.org/10.1117/12.644521

Portable real-time fluorescence cytometry of microscale cell culture analog devices. / Kim, Donghyun; Tatosian, Daniel A.; Shuler, Michael L.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2006. 60880X (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6088).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AB - A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.

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