Portable real-time fluorescence cytometry of microscale cell culture analog devices

Donghyun Kim, Daniel A. Tatosian, Michael L. Shuler

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6088
DOIs
Publication statusPublished - 2006 May 8
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV - San Jose, CA, United States
Duration: 2006 Jan 232006 Jan 25

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
CountryUnited States
CitySan Jose, CA
Period06/1/2306/1/25

Fingerprint

cytometry
Green Fluorescent Proteins
Cell culture
microbalances
Cell Culture Techniques
Fluorescence
analogs
Proteins
Photobleaching
Equipment and Supplies
fluorescence
Daunorubicin
Doxycycline
Light emitting diodes
Light sources
Microscopic examination
Genes
Cells
mice
proteins

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Kim, D., Tatosian, D. A., & Shuler, M. L. (2006). Portable real-time fluorescence cytometry of microscale cell culture analog devices. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6088). [60880X] https://doi.org/10.1117/12.644521
Kim, Donghyun ; Tatosian, Daniel A. ; Shuler, Michael L. / Portable real-time fluorescence cytometry of microscale cell culture analog devices. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6088 2006.
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Kim, D, Tatosian, DA & Shuler, ML 2006, Portable real-time fluorescence cytometry of microscale cell culture analog devices. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6088, 60880X, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, San Jose, CA, United States, 06/1/23. https://doi.org/10.1117/12.644521

Portable real-time fluorescence cytometry of microscale cell culture analog devices. / Kim, Donghyun; Tatosian, Daniel A.; Shuler, Michael L.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6088 2006. 60880X.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Kim D, Tatosian DA, Shuler ML. Portable real-time fluorescence cytometry of microscale cell culture analog devices. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6088. 2006. 60880X https://doi.org/10.1117/12.644521