Portable real-time fluorescence cytometry of microscale cell culture analog devices

Donghyun Kim; Daniel A. Tatosian; Michael L. Shuler

doi:10.1117/12.644521

Portable real-time fluorescence cytometry of microscale cell culture analog devices

Donghyun Kim, Daniel A. Tatosian, Michael L. Shuler

Department of Electrical and Electronic Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.

Original language	English
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
DOIs	https://doi.org/10.1117/12.644521
Publication status	Published - 2006
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV - San Jose, CA, United States Duration: 2006 Jan 23 → 2006 Jan 25

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6088
ISSN (Print)	1605-7422

Other

Other	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
Country/Territory	United States
City	San Jose, CA
Period	06/1/23 → 06/1/25

Bibliographical note

Funding Information:
This study was supported by a grant from the National R&D Program for Cancer Control, Ministry of Health and Welfare, Republic of Korea (HA16C0015) (1720150).

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.644521

Cite this

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title = "Portable real-time fluorescence cytometry of microscale cell culture analog devices",

abstract = "A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.",

author = "Donghyun Kim and Tatosian, {Daniel A.} and Shuler, {Michael L.}",

note = "Funding Information: This study was supported by a grant from the National R&D Program for Cancer Control, Ministry of Health and Welfare, Republic of Korea (HA16C0015) (1720150).; Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV ; Conference date: 23-01-2006 Through 25-01-2006",

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series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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Kim, D, Tatosian, DA & Shuler, ML 2006, Portable real-time fluorescence cytometry of microscale cell culture analog devices. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE., 60880X, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, San Jose, CA, United States, 06/1/23. https://doi.org/10.1117/12.644521

Portable real-time fluorescence cytometry of microscale cell culture analog devices. / Kim, Donghyun; Tatosian, Daniel A.; Shuler, Michael L.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2006. 60880X (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6088).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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Portable real-time fluorescence cytometry of microscale cell culture analog devices

Abstract

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Bibliographical note

All Science Journal Classification (ASJC) codes

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