Portable real-time fluorescence cytometry of microscale cell culture analog devices

Donghyun Kim, Daniel A. Tatosian, Michael L. Shuler

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
DOIs
Publication statusPublished - 2006 May 8
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV - San Jose, CA, United States
Duration: 2006 Jan 232006 Jan 25

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6088
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
CountryUnited States
CitySan Jose, CA
Period06/1/2306/1/25

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Kim, D., Tatosian, D. A., & Shuler, M. L. (2006). Portable real-time fluorescence cytometry of microscale cell culture analog devices. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE [60880X] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6088). https://doi.org/10.1117/12.644521