Positive and negative feedback regulatory loops of thiol-oxidative stress response mediated by an unstable isoform of σR in actinomycetes

Min Sik Kim, Mi Young Hahn, Yoobok Cho, Sangnae Cho, Jung Hye Roe

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Alternate sigma factors provide an effective way of diversifying bacterial gene expression in response to environmental changes. In Streptomyces coelicolor where more than 65 sigma factors are predicted, σR is the major regulator for response to thiol-oxidative stresses. σR becomes available when its bound antisigma factor RsrA is oxidized at sensitive cysteine thiols to form disulphide bonds. σR regulon includes genes for itself and multiple thiol-reducing systems, which constitute positive and negative feedback loops respectively. We found that the positive amplification loop involves an isoform of σRR') with an N-terminal extension of 55 amino acids, produced from an upstream start codon. A major difference between constitutive σR and inducible σR' is that the latter is markedly unstable (t1/2 ∼ 10 min) compared with the former (> 70 min). The rapid turnover of σR' is partly due to induced ClpP1/P2 proteases from the σR regulon. This represents a novel way of elaborating positive and negative feedback loops in a control circuit. Similar phenomenon may occur in other actinomycetes that harbour multiple start codons in the sigR homologous gene. We observed that sigH gene, the sigR orthologue in Mycobacterium smegmatis, produces an unstable larger isoform of σH upon induction by thiol-oxidative stress.

Original languageEnglish
Pages (from-to)815-825
Number of pages11
JournalMolecular Microbiology
Volume73
Issue number5
DOIs
Publication statusPublished - 2009 Sep 1

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Actinobacteria
Sulfhydryl Compounds
Protein Isoforms
Oxidative Stress
Sigma Factor
Regulon
Initiator Codon
Streptomyces coelicolor
Genes
Mycobacterium smegmatis
Bacterial Genes
Disulfides
Cysteine
Peptide Hydrolases
Gene Expression
Amino Acids

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

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abstract = "Alternate sigma factors provide an effective way of diversifying bacterial gene expression in response to environmental changes. In Streptomyces coelicolor where more than 65 sigma factors are predicted, σR is the major regulator for response to thiol-oxidative stresses. σR becomes available when its bound antisigma factor RsrA is oxidized at sensitive cysteine thiols to form disulphide bonds. σR regulon includes genes for itself and multiple thiol-reducing systems, which constitute positive and negative feedback loops respectively. We found that the positive amplification loop involves an isoform of σR (σR') with an N-terminal extension of 55 amino acids, produced from an upstream start codon. A major difference between constitutive σR and inducible σR' is that the latter is markedly unstable (t1/2 ∼ 10 min) compared with the former (> 70 min). The rapid turnover of σR' is partly due to induced ClpP1/P2 proteases from the σR regulon. This represents a novel way of elaborating positive and negative feedback loops in a control circuit. Similar phenomenon may occur in other actinomycetes that harbour multiple start codons in the sigR homologous gene. We observed that sigH gene, the sigR orthologue in Mycobacterium smegmatis, produces an unstable larger isoform of σH upon induction by thiol-oxidative stress.",
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Positive and negative feedback regulatory loops of thiol-oxidative stress response mediated by an unstable isoform of σR in actinomycetes. / Kim, Min Sik; Hahn, Mi Young; Cho, Yoobok; Cho, Sangnae; Roe, Jung Hye.

In: Molecular Microbiology, Vol. 73, No. 5, 01.09.2009, p. 815-825.

Research output: Contribution to journalArticle

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