Positive control for cytotoxicity evaluation of dental vinyl polysiloxane impression materials using sodium lauryl sulfate

Jae Sung Kwon, Sang Bae Lee, Kwang Mahn Kim, Kyoung Nam Kim

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2 Citations (Scopus)


OBJECTIVES: Vinyl polysiloxane (VPS) is elastomeric dental impression material which, despite having very few reports of adverse reactions, has shown high levels of cytotoxicity that is difficult to be interpreted without referencing to the positive control material. Therefore, in this study, positive control VPS was developed using sodium lauryl sulfate (SLS) for the reference of cytotoxicity test.

MATERIALS AND METHODS: The positive control VPS with SLS was formed with a different proportion of SLS (0, 1, 2, 4, 8 and 16 wt%) added to the base. The cytotoxicity test was then carried out using the extractions or dilutions of the extractions from each of the test samples using murine fibroblast cells (L929).

RESULTS: The final product of positive control VPS behaved similar to commercially available VPS; being initially liquid-like and then becoming rubber-like. Ion chromatography showed that the level of SLS released from the product increased as the proportion of added SLS increased, consequently resulting in an increased level of cytotoxicity. Also, the commercially available VPS was less cytotoxic than the positive control VPS with more or equal to 2 wt% of SLS. However, even the VPS with the highest SLS (16 wt%) did not cause oral mucosa irritation during the animal study.

CONCLUSIONS: The positive control VPS was successfully produced using SLS, which will be useful in terms of providing references during in vitro cytotoxicity testing.

Original languageEnglish
Pages (from-to)618-622
Number of pages5
JournalActa odontologica Scandinavica
Issue number8
Publication statusPublished - 2014 Nov 1

Bibliographical note

Funding Information:
This research was supported by the Dental Devices Testing & Evaluation Center, Yonsei University College of Dentistry and by a grant (14172MFDS334) from Ministry of Food and Drug Safety.

All Science Journal Classification (ASJC) codes

  • Dentistry(all)


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