Positive selection of the peripheral B cell repertoire in gut-associated lymphoid tissues

Kijong Rhee, Paul J. Jasper, Periannan Sethupathi, Malathy Shanmugam, Dennis Lanning, Katherine L. Knight

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Gut-associated lymphoid tissues (GALTs) interact with intestinal microflora to drive GALT development and diversify the primary antibody repertoire; however, the molecular mechanisms that link these events remain elusive. Alicia rabbits provide an excellent model to investigate the relationship between GALT, intestinal microflora, and modulation of the antibody repertoire. Most B cells in neonatal Alicia rabbits express VHn allotype immunoglobulin (Ig)M. Within weeks, the number of VHn B cells decreases, whereas V Ha allotype B cells increase in number and become predominant. We hypothesized that the repertoire shift from VHn to VHa B cells results from interactions between GALT and intestinal microflora. To test this hypothesis, we surgically removed organized GALT from newborn Alicia pups and ligated the appendix to sequester it from intestinal microflora. Flow cytometry and nucleotide sequence analyses revealed that the VHn to VHa repertoire shift did not occur, demonstrating the requirement for interactions between GALT and intestinal microflora in the selective expansion of VHa B cells. By comparing amino acid sequences of VHn and VHa Ig, we identified a putative VH ligand binding site for a bacterial or endogenous B cell superantigen. We propose that interaction of such a superantigen with VHa B cells results in their selective expansion.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalJournal of Experimental Medicine
Volume201
Issue number1
DOIs
Publication statusPublished - 2005 Jan 3

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Lymphoid Tissue
B-Lymphocytes
Superantigens
Rabbits
Antibodies
Cell Communication
Immunoglobulin M
Sequence Analysis
Immunoglobulins
Amino Acid Sequence
Flow Cytometry
Binding Sites
Gastrointestinal Microbiome
Ligands

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Rhee, Kijong ; Jasper, Paul J. ; Sethupathi, Periannan ; Shanmugam, Malathy ; Lanning, Dennis ; Knight, Katherine L. / Positive selection of the peripheral B cell repertoire in gut-associated lymphoid tissues. In: Journal of Experimental Medicine. 2005 ; Vol. 201, No. 1. pp. 55-62.
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Positive selection of the peripheral B cell repertoire in gut-associated lymphoid tissues. / Rhee, Kijong; Jasper, Paul J.; Sethupathi, Periannan; Shanmugam, Malathy; Lanning, Dennis; Knight, Katherine L.

In: Journal of Experimental Medicine, Vol. 201, No. 1, 03.01.2005, p. 55-62.

Research output: Contribution to journalArticle

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