Positron emitting magnetic nanoconstructs for PET/MR imaging

Santosh Aryal, Jaehong Key, Cinzia Stigliano, Melissa D. Landis, Daniel Y. Lee, Paolo Decuzzi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Hybrid PET/MRI scanners have the potential to provide fundamental molecular, cellular, and anatomic information essential for optimizing therapeutic and surgical interventions. However, their full utilization is currently limited by the lack of truly multi-modal contrast agents capable of exploiting the strengths of each modality. Here, we report on the development of long-circulating positron-emitting magnetic nanoconstructs (PEM) designed to image solid tumors for combined PET/MRI. PEMs are synthesized by a modified nano-precipitation method mixing poly(lactic-co-glycolic acid) (PLGA), lipids, and polyethylene glycol (PEG) chains with 5 nm iron oxide nanoparticles (USPIOs). PEM lipids are coupled with 1,4,7,10-tetraazacyclododecane-1,4,7,10- tetraacetic acid (DOTA) and subsequently chelated to 64Cu. PEMs show a diameter of 140 ± 7 nm and a transversal relaxivity r2 of 265.0 ± 10.0 (mM × s)-1, with a r2/r 1 ratio of 123. Using a murine xenograft model bearing human breast cancer cell line (MDA-MB-231), intravenously administered PEMs progressively accumulate in tumors reaching a maximum of 3.5 ± 0.25% ID/g tumor at 20 h post-injection. Correlation of PET and MRI signals revealed non-uniform intratumoral distribution of PEMs with focal areas of accumulation at the tumor periphery. These long-circulating PEMs with high transversal relaxivity and tumor accumulation may allow for detailed interrogation over multiple scales in a clinically relevant setting. Long-circulating positron-emitting magnetic nanoconstructs (PEM) are designed to image solid tumors for combined PET/MRI. These long-circulating PEMs with high transversal relaxivity and tumor accumulation may allow detailed interrogation over multiple scales in a clinically relevant setting.

Original languageEnglish
Pages (from-to)2688-2696
Number of pages9
JournalSmall
Volume10
Issue number13
DOIs
Publication statusPublished - 2014

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

Fingerprint Dive into the research topics of 'Positron emitting magnetic nanoconstructs for PET/MR imaging'. Together they form a unique fingerprint.

  • Cite this

    Aryal, S., Key, J., Stigliano, C., Landis, M. D., Lee, D. Y., & Decuzzi, P. (2014). Positron emitting magnetic nanoconstructs for PET/MR imaging. Small, 10(13), 2688-2696. https://doi.org/10.1002/smll.201303933