Possible role of light in the maintenance of EIN3/EIL1 stability in Arabidopsis seedlings

Jae Hoon Lee; Xing Wang Deng; Woo Taek Kim

doi:10.1016/j.bbrc.2006.09.074

Possible role of light in the maintenance of EIN3/EIL1 stability in Arabidopsis seedlings

Jae Hoon Lee, Xing Wang Deng, Woo Taek Kim

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32 Citations (Scopus)

Abstract

To examine the mechanism of EIN3-mediated gene expression by ethylene, the expression patterns of ethylene-inducible genes by ethylene were monitored in Col-0 and ethylene signaling mutants. In Col-0, the inducibility of ACC oxidase by ethylene in light-grown seedlings was much higher than in dark-grown seedlings. While the expression of ACC oxidase was highly increased by ethylene not only in Col-0 but in ein3-1 under light treatment, this pattern was completely abrogated in etiolated ein3-1 seedlings, suggesting the expression of EIN3-mediated ACC oxidase genes could be affected by light. To check if the level of EIN3 and EIL1 was regulated by light, cell-free degradation assays were performed. This resulted in the rapid degradation of these proteins within 1 h after adding dark-grown cell extracts and this degradation was retarded by light-grown extracts. Here, we propose that light may act as a negative regulator in the destabilization of EIN3/EIL1.

Original language	English
Pages (from-to)	484-491
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	350
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2006.09.074
Publication status	Published - 2006 Nov 17

Bibliographical note

Funding Information:
We are grateful to Dr. Joseph R. Ecker for providing eil1-1, eil1-2, ein3-1/eil1-1, and ein3-1/eil1-2 mutants. Several ethylene signaling mutants were kindly provided from the Arabidopsis Biological Resource Center. The authors thank Dr. Sunglan Chung (Department of Cell Biology, Yale University) for critical reading of the manuscript. This work was supported by grants from the Plant Diversity Research Center (21st Century Frontier Research Program funded by the Ministry of Science and Technology of the Korean government) and the Plant Metabolism Research Center at Kyung Hee University (Science Research Center Project No. R11-2000-081 from the Korea Science and Engineering Foundation). J.-H.L. was supported by the Post-doctoral Fellowship Program of Korea Research Foundation (KRF M01-2004-000-20090-0).

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2006.09.074

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title = "Possible role of light in the maintenance of EIN3/EIL1 stability in Arabidopsis seedlings",

abstract = "To examine the mechanism of EIN3-mediated gene expression by ethylene, the expression patterns of ethylene-inducible genes by ethylene were monitored in Col-0 and ethylene signaling mutants. In Col-0, the inducibility of ACC oxidase by ethylene in light-grown seedlings was much higher than in dark-grown seedlings. While the expression of ACC oxidase was highly increased by ethylene not only in Col-0 but in ein3-1 under light treatment, this pattern was completely abrogated in etiolated ein3-1 seedlings, suggesting the expression of EIN3-mediated ACC oxidase genes could be affected by light. To check if the level of EIN3 and EIL1 was regulated by light, cell-free degradation assays were performed. This resulted in the rapid degradation of these proteins within 1 h after adding dark-grown cell extracts and this degradation was retarded by light-grown extracts. Here, we propose that light may act as a negative regulator in the destabilization of EIN3/EIL1.",

author = "Lee, {Jae Hoon} and Deng, {Xing Wang} and Kim, {Woo Taek}",

note = "Funding Information: We are grateful to Dr. Joseph R. Ecker for providing eil1-1, eil1-2, ein3-1/eil1-1, and ein3-1/eil1-2 mutants. Several ethylene signaling mutants were kindly provided from the Arabidopsis Biological Resource Center. The authors thank Dr. Sunglan Chung (Department of Cell Biology, Yale University) for critical reading of the manuscript. This work was supported by grants from the Plant Diversity Research Center (21st Century Frontier Research Program funded by the Ministry of Science and Technology of the Korean government) and the Plant Metabolism Research Center at Kyung Hee University (Science Research Center Project No. R11-2000-081 from the Korea Science and Engineering Foundation). J.-H.L. was supported by the Post-doctoral Fellowship Program of Korea Research Foundation (KRF M01-2004-000-20090-0).",

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AU - Lee, Jae Hoon

AU - Deng, Xing Wang

AU - Kim, Woo Taek

N1 - Funding Information: We are grateful to Dr. Joseph R. Ecker for providing eil1-1, eil1-2, ein3-1/eil1-1, and ein3-1/eil1-2 mutants. Several ethylene signaling mutants were kindly provided from the Arabidopsis Biological Resource Center. The authors thank Dr. Sunglan Chung (Department of Cell Biology, Yale University) for critical reading of the manuscript. This work was supported by grants from the Plant Diversity Research Center (21st Century Frontier Research Program funded by the Ministry of Science and Technology of the Korean government) and the Plant Metabolism Research Center at Kyung Hee University (Science Research Center Project No. R11-2000-081 from the Korea Science and Engineering Foundation). J.-H.L. was supported by the Post-doctoral Fellowship Program of Korea Research Foundation (KRF M01-2004-000-20090-0).

PY - 2006/11/17

Y1 - 2006/11/17

N2 - To examine the mechanism of EIN3-mediated gene expression by ethylene, the expression patterns of ethylene-inducible genes by ethylene were monitored in Col-0 and ethylene signaling mutants. In Col-0, the inducibility of ACC oxidase by ethylene in light-grown seedlings was much higher than in dark-grown seedlings. While the expression of ACC oxidase was highly increased by ethylene not only in Col-0 but in ein3-1 under light treatment, this pattern was completely abrogated in etiolated ein3-1 seedlings, suggesting the expression of EIN3-mediated ACC oxidase genes could be affected by light. To check if the level of EIN3 and EIL1 was regulated by light, cell-free degradation assays were performed. This resulted in the rapid degradation of these proteins within 1 h after adding dark-grown cell extracts and this degradation was retarded by light-grown extracts. Here, we propose that light may act as a negative regulator in the destabilization of EIN3/EIL1.

AB - To examine the mechanism of EIN3-mediated gene expression by ethylene, the expression patterns of ethylene-inducible genes by ethylene were monitored in Col-0 and ethylene signaling mutants. In Col-0, the inducibility of ACC oxidase by ethylene in light-grown seedlings was much higher than in dark-grown seedlings. While the expression of ACC oxidase was highly increased by ethylene not only in Col-0 but in ein3-1 under light treatment, this pattern was completely abrogated in etiolated ein3-1 seedlings, suggesting the expression of EIN3-mediated ACC oxidase genes could be affected by light. To check if the level of EIN3 and EIL1 was regulated by light, cell-free degradation assays were performed. This resulted in the rapid degradation of these proteins within 1 h after adding dark-grown cell extracts and this degradation was retarded by light-grown extracts. Here, we propose that light may act as a negative regulator in the destabilization of EIN3/EIL1.

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Possible role of light in the maintenance of EIN3/EIL1 stability in Arabidopsis seedlings

Abstract

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