The restoration of damaged articular cartilage is a long-pursued goal in regenerative medicine. Chondrocyte-specific differentiation of mesenchymal stem cells (MSCs) may be an effective means of repairing damaged cartilage. We identified small molecule 6 with sulfonamide as an agent that promotes specific chondrogenic differentiation of human adipose-derived MSCs (hASCs). Unlike other chondrogenic differentiation media composed of various defined components, simply adding compound 6 into culture medium was sufficient to induce chondrogenesis in this study. In an animal osteoarthritis model, both the small molecule 6 and the 6-treated hASCs exhibited enhanced recovery of injured articular cartilage. This work provides new insight into MSC differentiation induced by small molecules and potential new therapeutic approaches for articular cartilage injury.
Bibliographical noteFunding Information:
This research was supported by a Korea Science and Engineering Foundation grant funded by the Korean government (MEST) (NRF-2015M3A9E6029519, NRF-2015M3A9E6029407, and NRF-2015M3A9E6029598), research fund of Catholic Kwandong University International St. Mary’s Hospital ( CKURF-201407080001 ), a grant from the Translational Research Center for Protein Function Control ( NRF-2009-0083522 ), the Ministry of Science, ICT and Future Planning ( NRF-2013M3A6A4072536 ), the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education ( NRF-2015R1A6A3A01020077 ), and the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea ( HI14C1324 ).
All Science Journal Classification (ASJC) codes
- Molecular Medicine
- Molecular Biology
- Pharmaceutical Science
- Drug Discovery
- Clinical Biochemistry
- Organic Chemistry