PPARγ activation induces CD36 expression and stimulates foam cell like changes in rVSMCs

Hyun Joung Lim; Seahyoung Lee; Kuy Sook Lee; Jin Hee Park; Yangsoo Jang; Eun Jig Lee; Hyun Young Park

doi:10.1016/j.prostaglandins.2006.06.006

PPARγ activation induces CD36 expression and stimulates foam cell like changes in rVSMCs

Hyun Joung Lim, Seahyoung Lee, Kuy Sook Lee, Jin Hee Park, Yangsoo Jang, Eun Jig Lee, Hyun Young Park

Department of Internal Medicine

Research output: Contribution to journal › Article

42 Citations (Scopus)

Abstract

The purpose of the present study was to determine the role of peroxisome proliferator-activated receptor gamma (PPARγ) activation in smooth muscle cell (SMC) derived form cell formation. Wild and mutant type PPARγ were delivered by adenovirus then activated with troglitazone. The result of Oil Red O staining and FACS analysis showed that PPARγ activation induced lipid accumulation in rVSMCs. Furthermore, PPARγ activation reduced SMC marker genes such as α-actin while induced adipocyte differentiation marker genes and lipid metabolism-related genes as evidenced by RT-PCR and fluorescent immunocytochemistry. All these data demonstrate that PPARγ activation can drive foam cell like change in rVSMCs. Our results strongly suggest that PPARγ expression induces CD36 expression and adipocyte differentiation gene activation in the process of atherosclerosis and might be one of the crucial events in SMC derived foam cell formation.

Original language	English
Pages (from-to)	165-174
Number of pages	10
Journal	Prostaglandins and Other Lipid Mediators
Volume	80
Issue number	3-4
DOIs	https://doi.org/10.1016/j.prostaglandins.2006.06.006
Publication status	Published - 2006 Sep

All Science Journal Classification (ASJC) codes

Biochemistry
Physiology
Pharmacology
Cell Biology

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10.1016/j.prostaglandins.2006.06.006

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title = "PPARγ activation induces CD36 expression and stimulates foam cell like changes in rVSMCs",

abstract = "The purpose of the present study was to determine the role of peroxisome proliferator-activated receptor gamma (PPARγ) activation in smooth muscle cell (SMC) derived form cell formation. Wild and mutant type PPARγ were delivered by adenovirus then activated with troglitazone. The result of Oil Red O staining and FACS analysis showed that PPARγ activation induced lipid accumulation in rVSMCs. Furthermore, PPARγ activation reduced SMC marker genes such as α-actin while induced adipocyte differentiation marker genes and lipid metabolism-related genes as evidenced by RT-PCR and fluorescent immunocytochemistry. All these data demonstrate that PPARγ activation can drive foam cell like change in rVSMCs. Our results strongly suggest that PPARγ expression induces CD36 expression and adipocyte differentiation gene activation in the process of atherosclerosis and might be one of the crucial events in SMC derived foam cell formation.",

author = "Lim, {Hyun Joung} and Seahyoung Lee and Lee, {Kuy Sook} and Park, {Jin Hee} and Yangsoo Jang and Lee, {Eun Jig} and Park, {Hyun Young}",

year = "2006",

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T1 - PPARγ activation induces CD36 expression and stimulates foam cell like changes in rVSMCs

AU - Lim, Hyun Joung

AU - Lee, Seahyoung

AU - Lee, Kuy Sook

AU - Park, Jin Hee

AU - Jang, Yangsoo

AU - Lee, Eun Jig

AU - Park, Hyun Young

PY - 2006/9

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N2 - The purpose of the present study was to determine the role of peroxisome proliferator-activated receptor gamma (PPARγ) activation in smooth muscle cell (SMC) derived form cell formation. Wild and mutant type PPARγ were delivered by adenovirus then activated with troglitazone. The result of Oil Red O staining and FACS analysis showed that PPARγ activation induced lipid accumulation in rVSMCs. Furthermore, PPARγ activation reduced SMC marker genes such as α-actin while induced adipocyte differentiation marker genes and lipid metabolism-related genes as evidenced by RT-PCR and fluorescent immunocytochemistry. All these data demonstrate that PPARγ activation can drive foam cell like change in rVSMCs. Our results strongly suggest that PPARγ expression induces CD36 expression and adipocyte differentiation gene activation in the process of atherosclerosis and might be one of the crucial events in SMC derived foam cell formation.

AB - The purpose of the present study was to determine the role of peroxisome proliferator-activated receptor gamma (PPARγ) activation in smooth muscle cell (SMC) derived form cell formation. Wild and mutant type PPARγ were delivered by adenovirus then activated with troglitazone. The result of Oil Red O staining and FACS analysis showed that PPARγ activation induced lipid accumulation in rVSMCs. Furthermore, PPARγ activation reduced SMC marker genes such as α-actin while induced adipocyte differentiation marker genes and lipid metabolism-related genes as evidenced by RT-PCR and fluorescent immunocytochemistry. All these data demonstrate that PPARγ activation can drive foam cell like change in rVSMCs. Our results strongly suggest that PPARγ expression induces CD36 expression and adipocyte differentiation gene activation in the process of atherosclerosis and might be one of the crucial events in SMC derived foam cell formation.

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