Abstract
Peroxisome proliferator-activated receptor-gamma (PPARy), a member of the nuclear receptor of ligand-activated transcription factors, plays a key role in lipid and glucose metabolism or adipocytes differentiation. A lignan compound was isolated from mace (the aril of Myristica fragrans Houtt.) as a PPARγ ligand, which was identified as fragrin A or 2-(4-allyl-2,6-dimethoxyphenoxy)-l- (4-hydroxy-3-methoxyphenyl)-propane. To ascertain whether fragrin A has PPARy ligand-binding activity, it was performed that GAL-4/PPARγ transactivation assay. PPARy ligand-binding activity of fragrin A increased 4.7, 6.6, and 7.3-fold at 3, 5, and 10 μM, respectively, when compared with a vehicle control. Fragrin A also enhanced adipocytes differentiation and increased the expression of PPARy target genes such as adipocytes fatty acid-binding protein (aP2), lipoprotein lipase (LPL), and phosphoenol pyruvate carboxykinase (PEPCK). Furthermore, it significantly increased the expression level of glucose transporter 4 (GLUT4). These results indicate that fragrin A can be developed as a PPARy agonist for the improvement of insulin resistance associated with type 2 diabetes.
| Original language | English |
|---|---|
| Pages (from-to) | 1146-1150 |
| Number of pages | 5 |
| Journal | Food Science and Biotechnology |
| Volume | 17 |
| Issue number | 6 |
| Publication status | Published - 2008 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology
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PPARγ ligand-binding activity of fragrin a isolated from mace (the aril of Myristica fragrans Houtt.). / Lee, Jae Young; Kim, Ba Reum; Oh, Hyun In; Shen, Lingai; Kim, Naeung Bae; Hwang, Jae Kwan.
In: Food Science and Biotechnology, Vol. 17, No. 6, 01.12.2008, p. 1146-1150.Research output: Contribution to journal › Article
TY - JOUR
T1 - PPARγ ligand-binding activity of fragrin a isolated from mace (the aril of Myristica fragrans Houtt.)
AU - Lee, Jae Young
AU - Kim, Ba Reum
AU - Oh, Hyun In
AU - Shen, Lingai
AU - Kim, Naeung Bae
AU - Hwang, Jae Kwan
PY - 2008/12/1
Y1 - 2008/12/1
N2 - Peroxisome proliferator-activated receptor-gamma (PPARy), a member of the nuclear receptor of ligand-activated transcription factors, plays a key role in lipid and glucose metabolism or adipocytes differentiation. A lignan compound was isolated from mace (the aril of Myristica fragrans Houtt.) as a PPARγ ligand, which was identified as fragrin A or 2-(4-allyl-2,6-dimethoxyphenoxy)-l- (4-hydroxy-3-methoxyphenyl)-propane. To ascertain whether fragrin A has PPARy ligand-binding activity, it was performed that GAL-4/PPARγ transactivation assay. PPARy ligand-binding activity of fragrin A increased 4.7, 6.6, and 7.3-fold at 3, 5, and 10 μM, respectively, when compared with a vehicle control. Fragrin A also enhanced adipocytes differentiation and increased the expression of PPARy target genes such as adipocytes fatty acid-binding protein (aP2), lipoprotein lipase (LPL), and phosphoenol pyruvate carboxykinase (PEPCK). Furthermore, it significantly increased the expression level of glucose transporter 4 (GLUT4). These results indicate that fragrin A can be developed as a PPARy agonist for the improvement of insulin resistance associated with type 2 diabetes.
AB - Peroxisome proliferator-activated receptor-gamma (PPARy), a member of the nuclear receptor of ligand-activated transcription factors, plays a key role in lipid and glucose metabolism or adipocytes differentiation. A lignan compound was isolated from mace (the aril of Myristica fragrans Houtt.) as a PPARγ ligand, which was identified as fragrin A or 2-(4-allyl-2,6-dimethoxyphenoxy)-l- (4-hydroxy-3-methoxyphenyl)-propane. To ascertain whether fragrin A has PPARy ligand-binding activity, it was performed that GAL-4/PPARγ transactivation assay. PPARy ligand-binding activity of fragrin A increased 4.7, 6.6, and 7.3-fold at 3, 5, and 10 μM, respectively, when compared with a vehicle control. Fragrin A also enhanced adipocytes differentiation and increased the expression of PPARy target genes such as adipocytes fatty acid-binding protein (aP2), lipoprotein lipase (LPL), and phosphoenol pyruvate carboxykinase (PEPCK). Furthermore, it significantly increased the expression level of glucose transporter 4 (GLUT4). These results indicate that fragrin A can be developed as a PPARy agonist for the improvement of insulin resistance associated with type 2 diabetes.
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M3 - Article
AN - SCOPUS:61349105410
VL - 17
SP - 1146
EP - 1150
JO - Food Science and Biotechnology
JF - Food Science and Biotechnology
SN - 1226-7708
IS - 6
ER -