PPARγ ligand-binding activity of fragrin a isolated from mace (the aril of Myristica fragrans Houtt.)

Jae Young Lee, Ba Reum Kim, Hyun In Oh, Lingai Shen, Naeung Bae Kim, Jae Kwan Hwang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Peroxisome proliferator-activated receptor-gamma (PPARy), a member of the nuclear receptor of ligand-activated transcription factors, plays a key role in lipid and glucose metabolism or adipocytes differentiation. A lignan compound was isolated from mace (the aril of Myristica fragrans Houtt.) as a PPARγ ligand, which was identified as fragrin A or 2-(4-allyl-2,6-dimethoxyphenoxy)-l- (4-hydroxy-3-methoxyphenyl)-propane. To ascertain whether fragrin A has PPARy ligand-binding activity, it was performed that GAL-4/PPARγ transactivation assay. PPARy ligand-binding activity of fragrin A increased 4.7, 6.6, and 7.3-fold at 3, 5, and 10 μM, respectively, when compared with a vehicle control. Fragrin A also enhanced adipocytes differentiation and increased the expression of PPARy target genes such as adipocytes fatty acid-binding protein (aP2), lipoprotein lipase (LPL), and phosphoenol pyruvate carboxykinase (PEPCK). Furthermore, it significantly increased the expression level of glucose transporter 4 (GLUT4). These results indicate that fragrin A can be developed as a PPARy agonist for the improvement of insulin resistance associated with type 2 diabetes.

Original languageEnglish
Pages (from-to)1146-1150
Number of pages5
JournalFood Science and Biotechnology
Volume17
Issue number6
Publication statusPublished - 2008 Dec 1

Fingerprint

omega-Chloroacetophenone
mace
Myristica fragrans
Peroxisome Proliferator-Activated Receptors
adipocytes
Adipocytes
Ligands
fatty acid-binding proteins
Fatty Acid-Binding Proteins
Lignans
Propane
glucose transporters
propane
lipoprotein lipase
Facilitative Glucose Transport Proteins
Lipoprotein Lipase
PPAR gamma
lignans
transcriptional activation
Cytoplasmic and Nuclear Receptors

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

Cite this

Lee, Jae Young ; Kim, Ba Reum ; Oh, Hyun In ; Shen, Lingai ; Kim, Naeung Bae ; Hwang, Jae Kwan. / PPARγ ligand-binding activity of fragrin a isolated from mace (the aril of Myristica fragrans Houtt.). In: Food Science and Biotechnology. 2008 ; Vol. 17, No. 6. pp. 1146-1150.
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PPARγ ligand-binding activity of fragrin a isolated from mace (the aril of Myristica fragrans Houtt.). / Lee, Jae Young; Kim, Ba Reum; Oh, Hyun In; Shen, Lingai; Kim, Naeung Bae; Hwang, Jae Kwan.

In: Food Science and Biotechnology, Vol. 17, No. 6, 01.12.2008, p. 1146-1150.

Research output: Contribution to journalArticle

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