Practical algorisms for PCR-RFLP-based genotyping of Echinococcus Granulosus sensu lato

Hye Jin Kim, Taisoon Yong, Myeong Heon Shin, Kyu Jae Lee, Gab Man Park, Uktamjon Suvonkulov, Dmitriy Kovalenko, Hak Sun Yu

Research output: Contribution to journalArticle

Abstract

Echinococcus granulosus sensu lato (s.l.) is a causative agent of cystic echinococcosis or cystic hydatid disease in humans and domestic and wild animals. The disease is a serious health problem in countries associated with poverty and poor hygiene practices, particularly in livestock raising. We introduced a practical algorism for genotyping the parasite, which may be useful to many developing countries. To evaluate the efficiency of the algorism, we genotyped 3 unknown strains isolated from human patients. We found that unknowns 1 and 3 were included in G1, G2, and G3 genotypes group and unknown 2 was included in G4 genotype (Echinococcus equinus) according to the algorisms. We confirmed these results by sequencing the 3 unknown isolates cox1 and nad1 PCR products. In conclusion, these new algorisms are very fast genotype identification tools that are suitable for evaluating E. granulosus s.l. isolated from livestock or livestock holders, particularly in developing countries.

Original languageEnglish
Pages (from-to)679-684
Number of pages6
JournalKorean Journal of Parasitology
Volume55
Issue number6
DOIs
Publication statusPublished - 2017 Dec 1

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Echinococcus granulosus
Livestock
Restriction Fragment Length Polymorphisms
Genotype
Polymerase Chain Reaction
Developing Countries
Echinococcus
Wild Animals
Echinococcosis
Domestic Animals
Poverty
Hygiene
Parasites
Health

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Kim, Hye Jin ; Yong, Taisoon ; Shin, Myeong Heon ; Lee, Kyu Jae ; Park, Gab Man ; Suvonkulov, Uktamjon ; Kovalenko, Dmitriy ; Yu, Hak Sun. / Practical algorisms for PCR-RFLP-based genotyping of Echinococcus Granulosus sensu lato. In: Korean Journal of Parasitology. 2017 ; Vol. 55, No. 6. pp. 679-684.
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Kim, HJ, Yong, T, Shin, MH, Lee, KJ, Park, GM, Suvonkulov, U, Kovalenko, D & Yu, HS 2017, 'Practical algorisms for PCR-RFLP-based genotyping of Echinococcus Granulosus sensu lato', Korean Journal of Parasitology, vol. 55, no. 6, pp. 679-684. https://doi.org/10.3347/kjp.2017.55.6.679

Practical algorisms for PCR-RFLP-based genotyping of Echinococcus Granulosus sensu lato. / Kim, Hye Jin; Yong, Taisoon; Shin, Myeong Heon; Lee, Kyu Jae; Park, Gab Man; Suvonkulov, Uktamjon; Kovalenko, Dmitriy; Yu, Hak Sun.

In: Korean Journal of Parasitology, Vol. 55, No. 6, 01.12.2017, p. 679-684.

Research output: Contribution to journalArticle

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AU - Kim, Hye Jin

AU - Yong, Taisoon

AU - Shin, Myeong Heon

AU - Lee, Kyu Jae

AU - Park, Gab Man

AU - Suvonkulov, Uktamjon

AU - Kovalenko, Dmitriy

AU - Yu, Hak Sun

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