Precision and accuracy of the analog mean-delay method for high-speed fluorescence lifetime measurement

Young Jae Won, Won Taek Han, Dug Young Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The analog mean-delay (AMD) method is a new alternative method to measure the lifetime of a fluorescence molecule. Because of its powerful advantages of accurate lifetime determination, good photon economy, and a high photon detection rate, the AMD method is considered to be very suitable for high-speed confocal fluorescence lifetime imaging microscopy (FLIM). For the practical usage of the AMD method in FLIM (AMD-FLIM), detailed study on various experimental conditions and parameters that affect the precision and the accuracy of the AMD method is required. In this paper, we present the relation between the precision and accuracy of the lifetime versus iteration number in the AMD method, the best cutoff frequency of a low-pass filter used in the AMD-FLIM system for a given fluorophore, and the optimum position and width of the integration window by using Monte Carlo simulations and a series of AMD-FLIM experiments.

Original languageEnglish
Pages (from-to)2026-2032
Number of pages7
JournalJournal of the Optical Society of America A: Optics and Image Science, and Vision
Volume28
Issue number10
DOIs
Publication statusPublished - 2011 Oct

Fingerprint

Fluorescence
high speed
analogs
Microscopic examination
life (durability)
fluorescence
Imaging techniques
microscopy
Photons
Fluorophores
Low pass filters
Cutoff frequency
low pass filters
photons
economy
iteration
Molecules
cut-off
Experiments
molecules

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Computer Vision and Pattern Recognition

Cite this

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