Prediction of the binding site on E1 in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus

Hyo il Jung, Richard N. Perham

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The β-subunit (E1β) of the pyruvate decarboxylase (E1, α2β2) component of the Bacillus stearothermophilus pyruvate dehydrogenase complex was comparatively modelled based on the crystal structures of the homologous 2-oxoisovalerate decarboxylase of Pseudomonas putida and Homo sapiens. Based on this homology modelling, alanine-scanning mutagenesis studies revealed that the negatively charged side chain of Glu285 and the hydrophobic side chain of Phe324 are of particular importance in the interaction with the peripheral subunit-binding domain of the dihydrolipoyl acetyltransferase component of the complex. These results help to identify the site of interaction on the E1β subunit and are consistent with thermodynamic evidence of a mixture of electrostatic and hydrophobic interactions being involved.

Original languageEnglish
Pages (from-to)405-410
Number of pages6
JournalFEBS Letters
Volume555
Issue number2
DOIs
Publication statusPublished - 2003 Dec 4

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Dihydrolipoyllysine-Residue Acetyltransferase
Pyruvate Decarboxylase
Multienzyme Complexes
Pyruvate Dehydrogenase Complex
Geobacillus stearothermophilus
Mutagenesis
Pseudomonas putida
Carboxy-Lyases
Bacilli
Static Electricity
Pyruvic Acid
Hydrophobic and Hydrophilic Interactions
Thermodynamics
Alanine
Electrostatics
Oxidoreductases
Crystal structure
Binding Sites
Scanning
alpha-ketoisovalerate

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

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Prediction of the binding site on E1 in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. / Jung, Hyo il; Perham, Richard N.

In: FEBS Letters, Vol. 555, No. 2, 04.12.2003, p. 405-410.

Research output: Contribution to journalArticle

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