Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow-derived cells and a hybrid biodegradable polymer scaffold

Seung-Woo Cho, Oju Jeon, Joung Eun Lim, So Jung Gwak, Sang Soo Kim, Cha Yong Choi, Dong Ik Kim, Byung Soo Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Objective: Currently available synthetic polymer vascular patches used in cardiovascular surgery have shown serious shortcomings, including thrombosis, calcification, infection, and lack of growth potential. These problems may be avoided by vascular patches tissue-engineered with autologous stem cells and biodegradable polymeric materials. The objective of this study was to develop a tissue-engineered vascular patch by using autologous bone marrow-derived cells (BMCs) and a hybrid biodegradable polymer scaffold. Methods: Hybrid biodegradable polymer scaffolds were fabricated from poly(lactide-co-ε-caprolactone) (PLCL) copolymer reinforced with poly(glycolic acid) (PGA) fibers. Canine bone marrow mononuclear cells were induced in vitro to differentiate into vascular smooth muscle cells and endothelial cells. Tissue-engineered vascular patches (15 mm wide × 30 mm long) were fabricated by seeding vascular cells onto PGA/PLCL scaffolds and implanted into the inferior vena cava of bone marrow donor dogs. Results: Compared with PLCL scaffolds, PGA/PLCL scaffolds exhibited tensile mechanical properties more similar to those of dog inferior vena cava. Eight weeks after implantation of vascular patches tissue-engineered with BMCs and PGA/PLCL scaffolds, the vascular patches remained patent with no sign of thrombosis, stenosis, or dilatation. Histological, immunohistochemical, and scanning electron microscopic analyses of the retrieved vascular patches revealed regeneration of endothelium and smooth muscle, as well as the presence of collagen. Calcium deposition on tissue-engineered vascular patches was not significantly different from that on native blood vessels. Immunofluorescent double staining confirmed that implanted BMCs survived after implantation and contributed to regeneration of endothelium and vascular smooth muscle in the implanted vascular patches. Conclusions: This study demonstrates that vascular patches can be tissue-engineered with autologous BMCs and hybrid biodegradable polymer scaffolds.

Original languageEnglish
Pages (from-to)1329-1340
Number of pages12
JournalJournal of Vascular Surgery
Volume44
Issue number6
DOIs
Publication statusPublished - 2006 Dec 1

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Tissue Engineering
Bone Marrow Cells
Blood Vessels
Polymers
Prostaglandins A
glycolic acid
Inferior Vena Cava
Vascular Smooth Muscle
Endothelium
Regeneration
Thrombosis
Dogs
Smooth Muscle Myocytes
Smooth Muscle
Canidae
Dilatation
Pathologic Constriction
Collagen
Stem Cells
Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Surgery
  • Cardiology and Cardiovascular Medicine

Cite this

Cho, Seung-Woo ; Jeon, Oju ; Lim, Joung Eun ; Gwak, So Jung ; Kim, Sang Soo ; Choi, Cha Yong ; Kim, Dong Ik ; Kim, Byung Soo. / Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow-derived cells and a hybrid biodegradable polymer scaffold. In: Journal of Vascular Surgery. 2006 ; Vol. 44, No. 6. pp. 1329-1340.
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abstract = "Objective: Currently available synthetic polymer vascular patches used in cardiovascular surgery have shown serious shortcomings, including thrombosis, calcification, infection, and lack of growth potential. These problems may be avoided by vascular patches tissue-engineered with autologous stem cells and biodegradable polymeric materials. The objective of this study was to develop a tissue-engineered vascular patch by using autologous bone marrow-derived cells (BMCs) and a hybrid biodegradable polymer scaffold. Methods: Hybrid biodegradable polymer scaffolds were fabricated from poly(lactide-co-ε-caprolactone) (PLCL) copolymer reinforced with poly(glycolic acid) (PGA) fibers. Canine bone marrow mononuclear cells were induced in vitro to differentiate into vascular smooth muscle cells and endothelial cells. Tissue-engineered vascular patches (15 mm wide × 30 mm long) were fabricated by seeding vascular cells onto PGA/PLCL scaffolds and implanted into the inferior vena cava of bone marrow donor dogs. Results: Compared with PLCL scaffolds, PGA/PLCL scaffolds exhibited tensile mechanical properties more similar to those of dog inferior vena cava. Eight weeks after implantation of vascular patches tissue-engineered with BMCs and PGA/PLCL scaffolds, the vascular patches remained patent with no sign of thrombosis, stenosis, or dilatation. Histological, immunohistochemical, and scanning electron microscopic analyses of the retrieved vascular patches revealed regeneration of endothelium and smooth muscle, as well as the presence of collagen. Calcium deposition on tissue-engineered vascular patches was not significantly different from that on native blood vessels. Immunofluorescent double staining confirmed that implanted BMCs survived after implantation and contributed to regeneration of endothelium and vascular smooth muscle in the implanted vascular patches. Conclusions: This study demonstrates that vascular patches can be tissue-engineered with autologous BMCs and hybrid biodegradable polymer scaffolds.",
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Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow-derived cells and a hybrid biodegradable polymer scaffold. / Cho, Seung-Woo; Jeon, Oju; Lim, Joung Eun; Gwak, So Jung; Kim, Sang Soo; Choi, Cha Yong; Kim, Dong Ik; Kim, Byung Soo.

In: Journal of Vascular Surgery, Vol. 44, No. 6, 01.12.2006, p. 1329-1340.

Research output: Contribution to journalArticle

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AU - Cho, Seung-Woo

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AU - Lim, Joung Eun

AU - Gwak, So Jung

AU - Kim, Sang Soo

AU - Choi, Cha Yong

AU - Kim, Dong Ik

AU - Kim, Byung Soo

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