Preparation and characterization of biodegradable anti-adhesive membrane for peritoneal wound healing

Si Nae Park, Han Jeong Jang, Yu Suk Choi, Jae Min Cha, Seo Yeon Son, Seung Hun Han, Jung Hyun Kim, Woo Jung Lee, Hwal Suh

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Postoperative adhesions remain a significant complication of abdominal surgery although the wide variety of physical barriers has been developed to reduce the incidence of adhesion. In this study, the bilayered composite membrane formed by the association of a methoxy poly (ethylene glycol)-poly (L-lactide-co-glycolide) (mPEG-PLGA) film and a crosslinked collagen-hyaluronic acid (Col-HA) membrane with fibronectin (FN) coating was prepared for promoting wound healing and providing tissue adhesion resistance simultaneously. In vitro adhesion test revealed that fibroblasts attached better on Col-HA membrane compared to those on mPEG-PLGA film, PLGA film or InterceedTM (oxidized cellulose) while mPEG-PLGA film had the lowest cell adhesive property. In confocal microscopic observation, the actin filaments were significantly further polymerized when 50 or 100 μg/cm3 fibronectin was incorporated on the COL-HA membranes. After 7-day culture, fibroblasts penetrated throughout the Col-HA-FN network and the cell density increased whereas very few cells were found attached on the surface of the mPEG-PLGA film. In vivo evaluation test showed that the composite membrane could remain during the critical period of peritoneal healing and did not provoke any inflammation or adverse tissue reaction.

Original languageEnglish
Pages (from-to)475-482
Number of pages8
JournalJournal of Materials Science: Materials in Medicine
Volume18
Issue number3
DOIs
Publication statusPublished - 2007 Mar 1

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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