Preparation and characterization of heparinized multi-walled carbon nanotubes

Tae Joon Park, Yong Seok Kim, Taewon Hwang, Patakamuri Govindaiah, Sung Wook Choi, Eunkyoung Kim, Keehoon Won, Sang Hyun Lee, Jung-Hyun Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The characteristics of heparinized multiwalled carbon nanotubes (MWNTs) were investigated in terms of the activated partial thromboplastin time (APTT) to verify the heparin activity, a carbazole assay was done to measure the content of the immobilized heparin, and the octanol-water partition coefficient was assessed to determine the lipophilicity. Two heparin-immobilized MWNTs were prepared to evaluate their differences. The first preparation method involved polymer-coated MWNTs with heparin indirectly center-point-attached. In the second approach, heparin was directly end-point-attached through its reducing end onto acid-treated MWNTs. The blood compatibility of MWNTs to which heparin was end-point-attached through its reducing end was greatly enhanced compared to that of the MWNTs onto which heparin was center-point-attached. The APTT and carbazole assay results demonstrated that heparinized MWNTs prepared through end-point attachment result in prolonged plasma-based anticoagulant activity. The blood compatibility of MWNTs heparinized by end-point attachment was not decreased up to the fourth pasteurization. Heparinized MWNTs were also studied using octanol-water partition, which should be useful for exploring heparinized MWNTs as drug carriers including delivery systems. The results of octanol/water partition on the design of heparinized MWNTs prepared by end-point attachment with a specific binding can facilitate the design of drug delivery carriers with high blood compatibility.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalProcess Biochemistry
Volume47
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

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Carbon Nanotubes
Multiwalled carbon nanotubes (MWCN)
Carbon nanotubes
Heparin
Octanols
Drug Carriers
Blood
Partial Thromboplastin Time
Thromboplastin
Water
Assays
Pasteurization
Drug delivery
Anticoagulants
Polymers

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Park, Tae Joon ; Kim, Yong Seok ; Hwang, Taewon ; Govindaiah, Patakamuri ; Choi, Sung Wook ; Kim, Eunkyoung ; Won, Keehoon ; Lee, Sang Hyun ; Kim, Jung-Hyun. / Preparation and characterization of heparinized multi-walled carbon nanotubes. In: Process Biochemistry. 2012 ; Vol. 47, No. 1. pp. 113-118.
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Park, TJ, Kim, YS, Hwang, T, Govindaiah, P, Choi, SW, Kim, E, Won, K, Lee, SH & Kim, J-H 2012, 'Preparation and characterization of heparinized multi-walled carbon nanotubes', Process Biochemistry, vol. 47, no. 1, pp. 113-118. https://doi.org/10.1016/j.procbio.2011.10.017

Preparation and characterization of heparinized multi-walled carbon nanotubes. / Park, Tae Joon; Kim, Yong Seok; Hwang, Taewon; Govindaiah, Patakamuri; Choi, Sung Wook; Kim, Eunkyoung; Won, Keehoon; Lee, Sang Hyun; Kim, Jung-Hyun.

In: Process Biochemistry, Vol. 47, No. 1, 01.01.2012, p. 113-118.

Research output: Contribution to journalArticle

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