Preparation and characterization of heparinized multi-walled carbon nanotubes

Tae Joon Park, Yong Seok Kim, Taewon Hwang, Patakamuri Govindaiah, Sung Wook Choi, Eunkyoung Kim, Keehoon Won, Sang Hyun Lee, Jung Hyun Kim

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Abstract

The characteristics of heparinized multiwalled carbon nanotubes (MWNTs) were investigated in terms of the activated partial thromboplastin time (APTT) to verify the heparin activity, a carbazole assay was done to measure the content of the immobilized heparin, and the octanol-water partition coefficient was assessed to determine the lipophilicity. Two heparin-immobilized MWNTs were prepared to evaluate their differences. The first preparation method involved polymer-coated MWNTs with heparin indirectly center-point-attached. In the second approach, heparin was directly end-point-attached through its reducing end onto acid-treated MWNTs. The blood compatibility of MWNTs to which heparin was end-point-attached through its reducing end was greatly enhanced compared to that of the MWNTs onto which heparin was center-point-attached. The APTT and carbazole assay results demonstrated that heparinized MWNTs prepared through end-point attachment result in prolonged plasma-based anticoagulant activity. The blood compatibility of MWNTs heparinized by end-point attachment was not decreased up to the fourth pasteurization. Heparinized MWNTs were also studied using octanol-water partition, which should be useful for exploring heparinized MWNTs as drug carriers including delivery systems. The results of octanol/water partition on the design of heparinized MWNTs prepared by end-point attachment with a specific binding can facilitate the design of drug delivery carriers with high blood compatibility.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalProcess Biochemistry
Volume47
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Park, T. J., Kim, Y. S., Hwang, T., Govindaiah, P., Choi, S. W., Kim, E., Won, K., Lee, S. H., & Kim, J. H. (2012). Preparation and characterization of heparinized multi-walled carbon nanotubes. Process Biochemistry, 47(1), 113-118. https://doi.org/10.1016/j.procbio.2011.10.017