Preparation and characterization of maltosyl-sucrose isomers produced by transglycosylation of maltogenic amylase from Bacillus stearothermophilus

Hye Young Lee, Myo Jeong Kim, Jin Sook Baek, Hee Seob Lee, Hyun Ju Cha, Soo-Bok Lee, Tae Wha Moon, Eun Seong Seo, Doman Kim, Cheon Seok Park, Kwan Hwa Park

Research output: Contribution to journalArticle

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Abstract

To develop a new transfer product of sucrose, sucrose was modified to maltosyl-sucrose using the transglycosylation activity of maltogenic amylase from Bacillus stearothermophilus (BSMA). The transglycosylation reaction was conducted with maltotriose and sucrose as the donor and acceptor, respectively. The presence of various sucrose transfer products was confirmed by thin layer chromatography (TLC) and high performance anion exchange chromatography (HPAEC). The sucrose transfer products were isolated by alkali-degradation followed by charcoal column chromatography using 20% (v/v) ethanol, then purified by ion exchange and Biogel P-2 gel permeation chromatographies. The structures of the major transfer products were determined to be 6 G -α-maltosyl- sucrose (maltosyl-sucrose 1) and 6 F -α-maltosyl-sucrose (maltosyl-sucrose 2) by LC-MS and 13 C NMR. The mixture of maltosyl-sucrose 1 and 2 showed low sweetness, high hygroscopicity, low Maillard reactivity, and high acid and heat stability. Furthermore, it had an inhibitory effect on mutansucrase and water-insoluble glucan formation. These results indicated that the mixture of maltosyl-sucrose 1 and 2 is a suitable sugar substitute useful for various food products.

Original languageEnglish
Pages (from-to)293-305
Number of pages13
JournalJournal of Molecular Catalysis B: Enzymatic
Volume26
Issue number3-6
DOIs
Publication statusPublished - 2003 Dec 1

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glucan 1,4-alpha-maltohydrolase
Geobacillus stearothermophilus
Amylases
Sugar (sucrose)
Bacilli
Isomers
Sucrose
Sugar substitutes
Chromatography
Ion exchange

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Bioengineering
  • Biochemistry
  • Process Chemistry and Technology

Cite this

Lee, Hye Young ; Kim, Myo Jeong ; Baek, Jin Sook ; Lee, Hee Seob ; Cha, Hyun Ju ; Lee, Soo-Bok ; Moon, Tae Wha ; Seo, Eun Seong ; Kim, Doman ; Park, Cheon Seok ; Park, Kwan Hwa. / Preparation and characterization of maltosyl-sucrose isomers produced by transglycosylation of maltogenic amylase from Bacillus stearothermophilus. In: Journal of Molecular Catalysis B: Enzymatic. 2003 ; Vol. 26, No. 3-6. pp. 293-305.
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abstract = "To develop a new transfer product of sucrose, sucrose was modified to maltosyl-sucrose using the transglycosylation activity of maltogenic amylase from Bacillus stearothermophilus (BSMA). The transglycosylation reaction was conducted with maltotriose and sucrose as the donor and acceptor, respectively. The presence of various sucrose transfer products was confirmed by thin layer chromatography (TLC) and high performance anion exchange chromatography (HPAEC). The sucrose transfer products were isolated by alkali-degradation followed by charcoal column chromatography using 20{\%} (v/v) ethanol, then purified by ion exchange and Biogel P-2 gel permeation chromatographies. The structures of the major transfer products were determined to be 6 G -α-maltosyl- sucrose (maltosyl-sucrose 1) and 6 F -α-maltosyl-sucrose (maltosyl-sucrose 2) by LC-MS and 13 C NMR. The mixture of maltosyl-sucrose 1 and 2 showed low sweetness, high hygroscopicity, low Maillard reactivity, and high acid and heat stability. Furthermore, it had an inhibitory effect on mutansucrase and water-insoluble glucan formation. These results indicated that the mixture of maltosyl-sucrose 1 and 2 is a suitable sugar substitute useful for various food products.",
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Preparation and characterization of maltosyl-sucrose isomers produced by transglycosylation of maltogenic amylase from Bacillus stearothermophilus. / Lee, Hye Young; Kim, Myo Jeong; Baek, Jin Sook; Lee, Hee Seob; Cha, Hyun Ju; Lee, Soo-Bok; Moon, Tae Wha; Seo, Eun Seong; Kim, Doman; Park, Cheon Seok; Park, Kwan Hwa.

In: Journal of Molecular Catalysis B: Enzymatic, Vol. 26, No. 3-6, 01.12.2003, p. 293-305.

Research output: Contribution to journalArticle

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AU - Lee, Hye Young

AU - Kim, Myo Jeong

AU - Baek, Jin Sook

AU - Lee, Hee Seob

AU - Cha, Hyun Ju

AU - Lee, Soo-Bok

AU - Moon, Tae Wha

AU - Seo, Eun Seong

AU - Kim, Doman

AU - Park, Cheon Seok

AU - Park, Kwan Hwa

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