Preparation and properties of immobilized amyloglucosidase on nonporous PS/PNaSS microspheres

Joon Taek Oh, Jung-Hyun Kim

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Nonporous polystyrene/poly(sodium styrene sulfonate) (PS/PNaSS) microspheres were used for immobilization of amyloglucosidase and the properties of immobilized enzyme was studied and compared with those of free enzyme. Sulfonated groups on the PS/PNaSS microspheres present a very simple, mild, and time-saving process for enzyme immobilization. Nonporous microspheres provide their surface for immobilization of enzyme and prevent the diffusion limitation problem in the pore. Despite the high concentration of bound enzyme the influence of immobilization on kinematic parameters, Km and Vmax, is relatively low compare to other porous supports. Simple and time-saving immobilization procedure as well as the effects of pH and temperature on immobilized enzyme also showed that the PS/PNaSS microspheres could be good support.

Original languageEnglish
Pages (from-to)356-361
Number of pages6
JournalEnzyme and Microbial Technology
Volume27
Issue number6
DOIs
Publication statusPublished - 2000 Sep 1

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Glucan 1,4-alpha-Glucosidase
Microspheres
Immobilization
Enzymes
Immobilized Enzymes
Enzyme immobilization
Styrene
Polystyrenes
Kinematics
Biomechanical Phenomena
Sodium
Temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "Nonporous polystyrene/poly(sodium styrene sulfonate) (PS/PNaSS) microspheres were used for immobilization of amyloglucosidase and the properties of immobilized enzyme was studied and compared with those of free enzyme. Sulfonated groups on the PS/PNaSS microspheres present a very simple, mild, and time-saving process for enzyme immobilization. Nonporous microspheres provide their surface for immobilization of enzyme and prevent the diffusion limitation problem in the pore. Despite the high concentration of bound enzyme the influence of immobilization on kinematic parameters, Km and Vmax, is relatively low compare to other porous supports. Simple and time-saving immobilization procedure as well as the effects of pH and temperature on immobilized enzyme also showed that the PS/PNaSS microspheres could be good support.",
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Preparation and properties of immobilized amyloglucosidase on nonporous PS/PNaSS microspheres. / Oh, Joon Taek; Kim, Jung-Hyun.

In: Enzyme and Microbial Technology, Vol. 27, No. 6, 01.09.2000, p. 356-361.

Research output: Contribution to journalArticle

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AU - Kim, Jung-Hyun

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AB - Nonporous polystyrene/poly(sodium styrene sulfonate) (PS/PNaSS) microspheres were used for immobilization of amyloglucosidase and the properties of immobilized enzyme was studied and compared with those of free enzyme. Sulfonated groups on the PS/PNaSS microspheres present a very simple, mild, and time-saving process for enzyme immobilization. Nonporous microspheres provide their surface for immobilization of enzyme and prevent the diffusion limitation problem in the pore. Despite the high concentration of bound enzyme the influence of immobilization on kinematic parameters, Km and Vmax, is relatively low compare to other porous supports. Simple and time-saving immobilization procedure as well as the effects of pH and temperature on immobilized enzyme also showed that the PS/PNaSS microspheres could be good support.

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