Autologous fat and synthetic and natural polymers have been used as materials and scaffolds in adipose tissue (AD) regeneration, combining various polymers and biological components, including extracellular matrix molecules, decellularized matrix, and adipose-derived stem cells, which have been studied to improve the adipogenesis ability of scaffolds. This study used human AD as the scaffold material, with a great source of biomaterials closest to the human body. We report a simple and facile scaffold fabrication method using a natural polymer, alginate, and human AD and confirmed the adipogenesis ability in vivo. Scaffold fabrication was performed by simply mixing human AD with alginate and cross-linking in a non-cytotoxic way, using CaCl2 solution. This method allows facile control of the shape and mechanical properties of the scaffold, which has great advantages in medical and aesthetic applications. In vitro experiments demonstrated the viability and secretion of adipokines in the scaffold. Four weeks of in vivo experiments showed that alginate protected human AD, which aided in AD survival, and that volume and shape were better preserved. Furthermore, we confirmed that our scaffold enhanced the adipose regeneration of the host animal and increased adipogenesis of the transplanted human AD by gene expression analysis.
Bibliographical noteFunding Information:
This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning and the Korea government (MSIT) (2020M3A914039045, 2016M3A9B4919711 and 2018M3A9E9071162 ).
© 2020 The Korean Society of Industrial and Engineering Chemistry
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)