Preparation of Chlorophyll-free Young Barley Leaf Extract Powders Using Supercritical Carbon Dioxide Modified with Cosolvent

Jin Hee Hong, Seungjoo Haam, Heaung Shig Oh, Giobin Lim, Jong Hoon Ryu

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we attempted to prepare chlorophyll-free young barley leaf extract powders using three different supercritical carbon dioxide (SCCO2) drying processes. The pure SCCO2 drying process for a methanolic extract solution resulted in a ~30% increase in the antioxidant (saponarin and isovitexin) content and a ~75% decrease in the chlorophyll content compared to a solvent evaporation method. The drying process with ethanol-modified SCCO2 increased the antioxidant content considerably and completely removed the chlorophylls. However, this process could not dry an aqueous ethanolic extract solution with a more than 30% water content. The ethanol-modified SCCO2 drying process that employed a periodic flush with pure SCCO2 was found to effectively dry an aqueous ethanolic extract solution with more than 30% water content.

Original languageEnglish
Pages (from-to)997-1006
Number of pages10
JournalBiotechnology and Bioprocess Engineering
Volume24
Issue number6
DOIs
Publication statusPublished - 2019 Nov 1

Bibliographical note

Funding Information:
This work was carried out with the support of “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ00950201)” Rural Development Administration, Republic of Korea. Publisher’s Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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