Preparation of micropatterned hydrogel substrate via surface graft polymerization combined with photolithography for biosensor application

Woojin Lee, Dongkil Choi, Yeol Lee, Dae Nyun Kim, Jin Won Park, Won-Gun Koh

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

This paper presents a simple method to create protein micropattern on the poly(ethylene glycol) (PEG) hydrogels through the surface graft polymerization and photolithography. The modification of the protein-repellent PEG hydrogel surface was achieved by a two-step process using immobilization of benzophenone on the PEG hydrogel as surface initiator and subsequent surface-initiated polymerization of acrylic acid by UV irradiation. Surface modification of PEG hydrogels was demonstrated with FTIR/ATR spectroscopy and XPS by confirming the presence of carboxyl groups in the poly(acrylic acid) (PAA). The photograft polymerization through the designed photomask produced well-defined, pH-responsive PAA micropatterns with diameters ranging from 50 to 300 μm on the PEG hydrogels. The size of PAA micropatterns was controlled by changing the environmental pH, such that a 300 μm diameter and 17 μm thick PAA micropattern at pH 4 swelled to 480 μm diameter and 80 μm thick at pH 7. Activation of the carboxyl groups in PAA allowed covalent immobilization of proteins only on the PAA micropatterns due to the nonadhesivity of PEG. Based on these results, biotin was micropatterned on the PEG hydrogels and binding of streptavidin was qualitatively and quantitatively investigated, demonstrating the possibility of micropatterned PEG hydrogels for various biosensor systems.

Original languageEnglish
Pages (from-to)841-849
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume129
Issue number2
DOIs
Publication statusPublished - 2008 Feb 22

Fingerprint

Hydrogel
Photolithography
photolithography
bioinstrumentation
Biosensors
Hydrogels
Grafts
Polyethylene glycols
polymerization
Polymerization
preparation
Substrates
carboxyl group
acrylic acid
proteins
immobilization
carbopol 940
biotin
photomasks
Proteins

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

@article{b0bb1fdef6f949c8854baa13a0afc142,
title = "Preparation of micropatterned hydrogel substrate via surface graft polymerization combined with photolithography for biosensor application",
abstract = "This paper presents a simple method to create protein micropattern on the poly(ethylene glycol) (PEG) hydrogels through the surface graft polymerization and photolithography. The modification of the protein-repellent PEG hydrogel surface was achieved by a two-step process using immobilization of benzophenone on the PEG hydrogel as surface initiator and subsequent surface-initiated polymerization of acrylic acid by UV irradiation. Surface modification of PEG hydrogels was demonstrated with FTIR/ATR spectroscopy and XPS by confirming the presence of carboxyl groups in the poly(acrylic acid) (PAA). The photograft polymerization through the designed photomask produced well-defined, pH-responsive PAA micropatterns with diameters ranging from 50 to 300 μm on the PEG hydrogels. The size of PAA micropatterns was controlled by changing the environmental pH, such that a 300 μm diameter and 17 μm thick PAA micropattern at pH 4 swelled to 480 μm diameter and 80 μm thick at pH 7. Activation of the carboxyl groups in PAA allowed covalent immobilization of proteins only on the PAA micropatterns due to the nonadhesivity of PEG. Based on these results, biotin was micropatterned on the PEG hydrogels and binding of streptavidin was qualitatively and quantitatively investigated, demonstrating the possibility of micropatterned PEG hydrogels for various biosensor systems.",
author = "Woojin Lee and Dongkil Choi and Yeol Lee and Kim, {Dae Nyun} and Park, {Jin Won} and Won-Gun Koh",
year = "2008",
month = "2",
day = "22",
doi = "10.1016/j.snb.2007.09.085",
language = "English",
volume = "129",
pages = "841--849",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "2",

}

Preparation of micropatterned hydrogel substrate via surface graft polymerization combined with photolithography for biosensor application. / Lee, Woojin; Choi, Dongkil; Lee, Yeol; Kim, Dae Nyun; Park, Jin Won; Koh, Won-Gun.

In: Sensors and Actuators, B: Chemical, Vol. 129, No. 2, 22.02.2008, p. 841-849.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Preparation of micropatterned hydrogel substrate via surface graft polymerization combined with photolithography for biosensor application

AU - Lee, Woojin

AU - Choi, Dongkil

AU - Lee, Yeol

AU - Kim, Dae Nyun

AU - Park, Jin Won

AU - Koh, Won-Gun

PY - 2008/2/22

Y1 - 2008/2/22

N2 - This paper presents a simple method to create protein micropattern on the poly(ethylene glycol) (PEG) hydrogels through the surface graft polymerization and photolithography. The modification of the protein-repellent PEG hydrogel surface was achieved by a two-step process using immobilization of benzophenone on the PEG hydrogel as surface initiator and subsequent surface-initiated polymerization of acrylic acid by UV irradiation. Surface modification of PEG hydrogels was demonstrated with FTIR/ATR spectroscopy and XPS by confirming the presence of carboxyl groups in the poly(acrylic acid) (PAA). The photograft polymerization through the designed photomask produced well-defined, pH-responsive PAA micropatterns with diameters ranging from 50 to 300 μm on the PEG hydrogels. The size of PAA micropatterns was controlled by changing the environmental pH, such that a 300 μm diameter and 17 μm thick PAA micropattern at pH 4 swelled to 480 μm diameter and 80 μm thick at pH 7. Activation of the carboxyl groups in PAA allowed covalent immobilization of proteins only on the PAA micropatterns due to the nonadhesivity of PEG. Based on these results, biotin was micropatterned on the PEG hydrogels and binding of streptavidin was qualitatively and quantitatively investigated, demonstrating the possibility of micropatterned PEG hydrogels for various biosensor systems.

AB - This paper presents a simple method to create protein micropattern on the poly(ethylene glycol) (PEG) hydrogels through the surface graft polymerization and photolithography. The modification of the protein-repellent PEG hydrogel surface was achieved by a two-step process using immobilization of benzophenone on the PEG hydrogel as surface initiator and subsequent surface-initiated polymerization of acrylic acid by UV irradiation. Surface modification of PEG hydrogels was demonstrated with FTIR/ATR spectroscopy and XPS by confirming the presence of carboxyl groups in the poly(acrylic acid) (PAA). The photograft polymerization through the designed photomask produced well-defined, pH-responsive PAA micropatterns with diameters ranging from 50 to 300 μm on the PEG hydrogels. The size of PAA micropatterns was controlled by changing the environmental pH, such that a 300 μm diameter and 17 μm thick PAA micropattern at pH 4 swelled to 480 μm diameter and 80 μm thick at pH 7. Activation of the carboxyl groups in PAA allowed covalent immobilization of proteins only on the PAA micropatterns due to the nonadhesivity of PEG. Based on these results, biotin was micropatterned on the PEG hydrogels and binding of streptavidin was qualitatively and quantitatively investigated, demonstrating the possibility of micropatterned PEG hydrogels for various biosensor systems.

UR - http://www.scopus.com/inward/record.url?scp=38949190285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38949190285&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2007.09.085

DO - 10.1016/j.snb.2007.09.085

M3 - Article

VL - 129

SP - 841

EP - 849

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

IS - 2

ER -