Preparation of nano-magnetite impregnated mesocellular foam composite with a Cu ligand for His-tagged enzyme immobilization

Eun Ji Woo; Hyuk Sung Kwon; Chang Ha Lee

doi:10.1016/j.cej.2015.03.123

Preparation of nano-magnetite impregnated mesocellular foam composite with a Cu ligand for His-tagged enzyme immobilization

Eun Ji Woo, Hyuk Sung Kwon, Chang Ha Lee

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A nano-sized magnetite (NSM) impregnated mesocellular foam (MCF) composite was synthesized to improve enzymatic activity and reusability in the immobilized state. 3-Chloropropyltriethoxy silane-di-(2-picolyl)amine was linked to the particle for cooperative complex formation with an enzyme through coordinated bonding with Cu (NSM/MCF-Cu). The NSM/MCF-Cu immobilized by histidine-tagged enzymes exhibited the high protein content and specific activity, showing an approximately 5 times higher protein content and 3 times higher specific activity compared to physically immobilized MCF particles. The NSM/MCF-Cu particles were recovered by magnetic separation and approximately 80% of the initial activity was retained even after 10 uses.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Chemical Engineering Journal
Volume	274
DOIs	https://doi.org/10.1016/j.cej.2015.03.123
Publication status	Published - 2015 Aug 5

Bibliographical note

Funding Information:
We acknowledge Dr. JungOh Ahn at the Korea Research Institute of Bioscience & Biotechnology (KRIBB) for the preparation of His-tagged B. stearothermophilus L1. This work is financially supported by Yonsei University , Republic of Korea.

Publisher Copyright:
© 2015 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Chemistry(all)
Environmental Chemistry
Chemical Engineering(all)
Industrial and Manufacturing Engineering

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10.1016/j.cej.2015.03.123

Cite this

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title = "Preparation of nano-magnetite impregnated mesocellular foam composite with a Cu ligand for His-tagged enzyme immobilization",

abstract = "A nano-sized magnetite (NSM) impregnated mesocellular foam (MCF) composite was synthesized to improve enzymatic activity and reusability in the immobilized state. 3-Chloropropyltriethoxy silane-di-(2-picolyl)amine was linked to the particle for cooperative complex formation with an enzyme through coordinated bonding with Cu (NSM/MCF-Cu). The NSM/MCF-Cu immobilized by histidine-tagged enzymes exhibited the high protein content and specific activity, showing an approximately 5 times higher protein content and 3 times higher specific activity compared to physically immobilized MCF particles. The NSM/MCF-Cu particles were recovered by magnetic separation and approximately 80% of the initial activity was retained even after 10 uses.",

author = "Woo, {Eun Ji} and Kwon, {Hyuk Sung} and Lee, {Chang Ha}",

note = "Funding Information: We acknowledge Dr. JungOh Ahn at the Korea Research Institute of Bioscience & Biotechnology (KRIBB) for the preparation of His-tagged B. stearothermophilus L1. This work is financially supported by Yonsei University , Republic of Korea. Publisher Copyright: {\textcopyright} 2015 Elsevier B.V.",

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AU - Woo, Eun Ji

AU - Kwon, Hyuk Sung

AU - Lee, Chang Ha

N1 - Funding Information: We acknowledge Dr. JungOh Ahn at the Korea Research Institute of Bioscience & Biotechnology (KRIBB) for the preparation of His-tagged B. stearothermophilus L1. This work is financially supported by Yonsei University , Republic of Korea. Publisher Copyright: © 2015 Elsevier B.V.

PY - 2015/8/5

Y1 - 2015/8/5

N2 - A nano-sized magnetite (NSM) impregnated mesocellular foam (MCF) composite was synthesized to improve enzymatic activity and reusability in the immobilized state. 3-Chloropropyltriethoxy silane-di-(2-picolyl)amine was linked to the particle for cooperative complex formation with an enzyme through coordinated bonding with Cu (NSM/MCF-Cu). The NSM/MCF-Cu immobilized by histidine-tagged enzymes exhibited the high protein content and specific activity, showing an approximately 5 times higher protein content and 3 times higher specific activity compared to physically immobilized MCF particles. The NSM/MCF-Cu particles were recovered by magnetic separation and approximately 80% of the initial activity was retained even after 10 uses.

AB - A nano-sized magnetite (NSM) impregnated mesocellular foam (MCF) composite was synthesized to improve enzymatic activity and reusability in the immobilized state. 3-Chloropropyltriethoxy silane-di-(2-picolyl)amine was linked to the particle for cooperative complex formation with an enzyme through coordinated bonding with Cu (NSM/MCF-Cu). The NSM/MCF-Cu immobilized by histidine-tagged enzymes exhibited the high protein content and specific activity, showing an approximately 5 times higher protein content and 3 times higher specific activity compared to physically immobilized MCF particles. The NSM/MCF-Cu particles were recovered by magnetic separation and approximately 80% of the initial activity was retained even after 10 uses.

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Preparation of nano-magnetite impregnated mesocellular foam composite with a Cu ligand for His-tagged enzyme immobilization

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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