Preparation of nano-magnetite impregnated mesocellular foam composite with a Cu ligand for His-tagged enzyme immobilization

Eun Ji Woo, Hyuk Sung Kwon, Chang Ha Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A nano-sized magnetite (NSM) impregnated mesocellular foam (MCF) composite was synthesized to improve enzymatic activity and reusability in the immobilized state. 3-Chloropropyltriethoxy silane-di-(2-picolyl)amine was linked to the particle for cooperative complex formation with an enzyme through coordinated bonding with Cu (NSM/MCF-Cu). The NSM/MCF-Cu immobilized by histidine-tagged enzymes exhibited the high protein content and specific activity, showing an approximately 5 times higher protein content and 3 times higher specific activity compared to physically immobilized MCF particles. The NSM/MCF-Cu particles were recovered by magnetic separation and approximately 80% of the initial activity was retained even after 10 uses.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalChemical Engineering Journal
Volume274
DOIs
Publication statusPublished - 2015 Aug 5

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

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