Preparation of nano-magnetite impregnated mesocellular foam composite with a Cu ligand for His-tagged enzyme immobilization

Eun Ji Woo, Hyuk Sung Kwon, Chang-Ha Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A nano-sized magnetite (NSM) impregnated mesocellular foam (MCF) composite was synthesized to improve enzymatic activity and reusability in the immobilized state. 3-Chloropropyltriethoxy silane-di-(2-picolyl)amine was linked to the particle for cooperative complex formation with an enzyme through coordinated bonding with Cu (NSM/MCF-Cu). The NSM/MCF-Cu immobilized by histidine-tagged enzymes exhibited the high protein content and specific activity, showing an approximately 5 times higher protein content and 3 times higher specific activity compared to physically immobilized MCF particles. The NSM/MCF-Cu particles were recovered by magnetic separation and approximately 80% of the initial activity was retained even after 10 uses.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalChemical Engineering Journal
Volume274
DOIs
Publication statusPublished - 2015 Aug 5

Fingerprint

Ferrosoferric Oxide
Enzyme immobilization
Magnetite
foam
immobilization
ligand
Foams
magnetite
Ligands
enzyme
Composite materials
Enzymes
Proteins
Silanes
Magnetic separation
protein
Reusability
Histidine
Amines
particle

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Chemistry(all)
  • Industrial and Manufacturing Engineering
  • Environmental Chemistry

Cite this

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abstract = "A nano-sized magnetite (NSM) impregnated mesocellular foam (MCF) composite was synthesized to improve enzymatic activity and reusability in the immobilized state. 3-Chloropropyltriethoxy silane-di-(2-picolyl)amine was linked to the particle for cooperative complex formation with an enzyme through coordinated bonding with Cu (NSM/MCF-Cu). The NSM/MCF-Cu immobilized by histidine-tagged enzymes exhibited the high protein content and specific activity, showing an approximately 5 times higher protein content and 3 times higher specific activity compared to physically immobilized MCF particles. The NSM/MCF-Cu particles were recovered by magnetic separation and approximately 80{\%} of the initial activity was retained even after 10 uses.",
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Preparation of nano-magnetite impregnated mesocellular foam composite with a Cu ligand for His-tagged enzyme immobilization. / Woo, Eun Ji; Kwon, Hyuk Sung; Lee, Chang-Ha.

In: Chemical Engineering Journal, Vol. 274, 05.08.2015, p. 1-8.

Research output: Contribution to journalArticle

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