A nano-sized magnetite (NSM) impregnated mesocellular foam (MCF) composite was synthesized to improve enzymatic activity and reusability in the immobilized state. 3-Chloropropyltriethoxy silane-di-(2-picolyl)amine was linked to the particle for cooperative complex formation with an enzyme through coordinated bonding with Cu (NSM/MCF-Cu). The NSM/MCF-Cu immobilized by histidine-tagged enzymes exhibited the high protein content and specific activity, showing an approximately 5 times higher protein content and 3 times higher specific activity compared to physically immobilized MCF particles. The NSM/MCF-Cu particles were recovered by magnetic separation and approximately 80% of the initial activity was retained even after 10 uses.
Bibliographical noteFunding Information:
We acknowledge Dr. JungOh Ahn at the Korea Research Institute of Bioscience & Biotechnology (KRIBB) for the preparation of His-tagged B. stearothermophilus L1. This work is financially supported by Yonsei University , Republic of Korea.
© 2015 Elsevier B.V.
All Science Journal Classification (ASJC) codes
- Environmental Chemistry
- Chemical Engineering(all)
- Industrial and Manufacturing Engineering