Preparation of peptide-loaded polymer microparticles using supercritical carbon dioxide

In Il Jung, Seoungjoo Haam, Giobin Lim, Jong Hoon Ryu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, peptide-loaded microparticles were prepared using an aerosol solvent extraction system (ASES) by employing supercritical carbon dioxide as an antisolvent. The effects of the molecular weight of poly(Llactide) (PLLA), poly(ethylene glycol) (PEG), the blocklength of methoxy poly(ethylene glycol)-b-poly(L-lactide) (mPEG-PLLA), the blending of PLLA and PEG, and the drug-to-polymer feed ratio on the formation of leuprolideacetate (LA)-loaded microparticles and their release characteristics were investigated. Scanning electron microscope observations showed that the LA-loaded polymer particles had a spherical morphology with a smooth surface. The entrapment efficiency of LA in the ASES-processed microparticles was found to be extremely high (about 99%), whereas the initial release rate of the LA-loaded microparticles was very low for PLLA. The release rate of LA was observed to increase as the PEG block length of mPEG-PLLA and/or the drug content in the microparticles increased. When PLLA was blended with PEG, the release rate of LA from the PLLA/PEG microparticles was significantly faster compared with the correspondingmPEG-PLLA copolymer

Original languageEnglish
Pages (from-to)185-194
Number of pages10
JournalBiotechnology and Bioprocess Engineering
Volume17
Issue number1
DOIs
Publication statusPublished - 2012 Feb 1

Fingerprint

Ethylene Glycol
Carbon Dioxide
Peptides
Polyethylene glycols
Carbon dioxide
Polymers
Solvent extraction
Aerosols
Ethylene glycol
Pharmaceutical Preparations
Electron microscopes
Copolymers
Molecular Weight
Molecular weight
Electrons
Scanning

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

Cite this

@article{1ab78a05847a4a26a504e784a95f407b,
title = "Preparation of peptide-loaded polymer microparticles using supercritical carbon dioxide",
abstract = "In this study, peptide-loaded microparticles were prepared using an aerosol solvent extraction system (ASES) by employing supercritical carbon dioxide as an antisolvent. The effects of the molecular weight of poly(Llactide) (PLLA), poly(ethylene glycol) (PEG), the blocklength of methoxy poly(ethylene glycol)-b-poly(L-lactide) (mPEG-PLLA), the blending of PLLA and PEG, and the drug-to-polymer feed ratio on the formation of leuprolideacetate (LA)-loaded microparticles and their release characteristics were investigated. Scanning electron microscope observations showed that the LA-loaded polymer particles had a spherical morphology with a smooth surface. The entrapment efficiency of LA in the ASES-processed microparticles was found to be extremely high (about 99{\%}), whereas the initial release rate of the LA-loaded microparticles was very low for PLLA. The release rate of LA was observed to increase as the PEG block length of mPEG-PLLA and/or the drug content in the microparticles increased. When PLLA was blended with PEG, the release rate of LA from the PLLA/PEG microparticles was significantly faster compared with the correspondingmPEG-PLLA copolymer",
author = "Jung, {In Il} and Seoungjoo Haam and Giobin Lim and Ryu, {Jong Hoon}",
year = "2012",
month = "2",
day = "1",
doi = "10.1007/s12257-011-0241-1",
language = "English",
volume = "17",
pages = "185--194",
journal = "Biotechnology and Bioprocess Engineering",
issn = "1226-8372",
publisher = "Korean Society for Biotechnology and Bioengineering",
number = "1",

}

Preparation of peptide-loaded polymer microparticles using supercritical carbon dioxide. / Jung, In Il; Haam, Seoungjoo; Lim, Giobin; Ryu, Jong Hoon.

In: Biotechnology and Bioprocess Engineering, Vol. 17, No. 1, 01.02.2012, p. 185-194.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Preparation of peptide-loaded polymer microparticles using supercritical carbon dioxide

AU - Jung, In Il

AU - Haam, Seoungjoo

AU - Lim, Giobin

AU - Ryu, Jong Hoon

PY - 2012/2/1

Y1 - 2012/2/1

N2 - In this study, peptide-loaded microparticles were prepared using an aerosol solvent extraction system (ASES) by employing supercritical carbon dioxide as an antisolvent. The effects of the molecular weight of poly(Llactide) (PLLA), poly(ethylene glycol) (PEG), the blocklength of methoxy poly(ethylene glycol)-b-poly(L-lactide) (mPEG-PLLA), the blending of PLLA and PEG, and the drug-to-polymer feed ratio on the formation of leuprolideacetate (LA)-loaded microparticles and their release characteristics were investigated. Scanning electron microscope observations showed that the LA-loaded polymer particles had a spherical morphology with a smooth surface. The entrapment efficiency of LA in the ASES-processed microparticles was found to be extremely high (about 99%), whereas the initial release rate of the LA-loaded microparticles was very low for PLLA. The release rate of LA was observed to increase as the PEG block length of mPEG-PLLA and/or the drug content in the microparticles increased. When PLLA was blended with PEG, the release rate of LA from the PLLA/PEG microparticles was significantly faster compared with the correspondingmPEG-PLLA copolymer

AB - In this study, peptide-loaded microparticles were prepared using an aerosol solvent extraction system (ASES) by employing supercritical carbon dioxide as an antisolvent. The effects of the molecular weight of poly(Llactide) (PLLA), poly(ethylene glycol) (PEG), the blocklength of methoxy poly(ethylene glycol)-b-poly(L-lactide) (mPEG-PLLA), the blending of PLLA and PEG, and the drug-to-polymer feed ratio on the formation of leuprolideacetate (LA)-loaded microparticles and their release characteristics were investigated. Scanning electron microscope observations showed that the LA-loaded polymer particles had a spherical morphology with a smooth surface. The entrapment efficiency of LA in the ASES-processed microparticles was found to be extremely high (about 99%), whereas the initial release rate of the LA-loaded microparticles was very low for PLLA. The release rate of LA was observed to increase as the PEG block length of mPEG-PLLA and/or the drug content in the microparticles increased. When PLLA was blended with PEG, the release rate of LA from the PLLA/PEG microparticles was significantly faster compared with the correspondingmPEG-PLLA copolymer

UR - http://www.scopus.com/inward/record.url?scp=84861033226&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861033226&partnerID=8YFLogxK

U2 - 10.1007/s12257-011-0241-1

DO - 10.1007/s12257-011-0241-1

M3 - Article

AN - SCOPUS:84861033226

VL - 17

SP - 185

EP - 194

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

SN - 1226-8372

IS - 1

ER -