Preparation of poly(ethylene glycol) hydrogels with different network structures for the application of enzyme immobilization

Dongkil Choi, Woojin Lee, Jin Won Park, Won-Gun Koh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this study, poly(ethylene glycol) (PEG)-based hydrogels having different network structures were synthesized by UV-initiated photopolymerization and used for the enzyme immobilization. PEGs with different molecular weight were acrylated by derivatizing both ends with acryloyl chloride and photopolymerization of PEG-diacrylate (PEG-DA) yielded crosslinked hydrogel network within 5 seconds. Attachment of acrylate groups and gelation were confirmed by ATR/FT-IR and FT-Raman spectroscopy. Network structures of hydrogels could be easily controlled by changing the molecular weight (MW) of PEG-DA and characterized by calculating molecular weight between crosslinks and mesh size from the swelling measurement. Synthesis of hydrogels with higher MW of PEG produced less crosslinked hydrogels having higher water content, larger value of Mc and mesh size, which resulted in enhanced mass transfer but loss of mechanical properties. For the enzyme immobilization, glucose oxidase (GOX) was immobilized inside PEG hydrogels by means of physical entrapment and covalent immobilization. Encapsulated GOX were covalently bound to PEG backbone using acryloyl-PEG-N-hydroxysuccinimide and maintained their activity over a week period without leakage. Kinetic study indicated that immobilized enzyme inside hydrogel prepared from higher MW of PEG possessed lower apparent Km (Michaelis-Menten constant) and higher activity.

Original languageEnglish
Pages (from-to)345-356
Number of pages12
JournalBio-Medical Materials and Engineering
Volume18
Issue number6
DOIs
Publication statusPublished - 2008 Dec 1

Fingerprint

Enzyme immobilization
Hydrogels
Polyethylene glycols
Molecular weight
Glucose Oxidase
Glucose oxidase
Photopolymerization
Hydrogel
Enzyme kinetics
Immobilized Enzymes
Gelation
Water content
Swelling
Raman spectroscopy
Mass transfer
Enzymes

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

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abstract = "In this study, poly(ethylene glycol) (PEG)-based hydrogels having different network structures were synthesized by UV-initiated photopolymerization and used for the enzyme immobilization. PEGs with different molecular weight were acrylated by derivatizing both ends with acryloyl chloride and photopolymerization of PEG-diacrylate (PEG-DA) yielded crosslinked hydrogel network within 5 seconds. Attachment of acrylate groups and gelation were confirmed by ATR/FT-IR and FT-Raman spectroscopy. Network structures of hydrogels could be easily controlled by changing the molecular weight (MW) of PEG-DA and characterized by calculating molecular weight between crosslinks and mesh size from the swelling measurement. Synthesis of hydrogels with higher MW of PEG produced less crosslinked hydrogels having higher water content, larger value of Mc and mesh size, which resulted in enhanced mass transfer but loss of mechanical properties. For the enzyme immobilization, glucose oxidase (GOX) was immobilized inside PEG hydrogels by means of physical entrapment and covalent immobilization. Encapsulated GOX were covalently bound to PEG backbone using acryloyl-PEG-N-hydroxysuccinimide and maintained their activity over a week period without leakage. Kinetic study indicated that immobilized enzyme inside hydrogel prepared from higher MW of PEG possessed lower apparent Km (Michaelis-Menten constant) and higher activity.",
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Preparation of poly(ethylene glycol) hydrogels with different network structures for the application of enzyme immobilization. / Choi, Dongkil; Lee, Woojin; Park, Jin Won; Koh, Won-Gun.

In: Bio-Medical Materials and Engineering, Vol. 18, No. 6, 01.12.2008, p. 345-356.

Research output: Contribution to journalArticle

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