Preservation of Human Saphenous Vein against Reactive Oxygen Species-induced Oxidative Stress by Green Tea Polyphenol Pretreatment

Dong Wook Han, Hwal Suh, Young Hwan Park, Bum Koo Cho, Suong Hyu Hyon, Jongchul Park

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The injurious effects of reactive oxygen species on venous tissues and the potential protective role played by green tea polyphenol (GTPP) on human saphenous veins were investigated. Oxidative stress was induced exogenously in the vein segments, either by adding 0.8 or 1.6 M of H2O2, or by using 80 or 160 U/L of xanthine oxidase in the presence of xanthine (0.5 mM). After incubation, the viability of the endothelial cells dissociated from veins and the histology of the veins were evaluated. Due to both types of treatment, a significant decrease in cellular viability, severe morphological changes in the veins, and extracellular structural damage were induced. The H2O2-induced alterations were prevented by preincubating the veins with either 0.5 or 1.0 mg/ml of GTPP for 1 h. When the oxidative stress was induced by xanthine oxidase, cellular viability and venous structure were preserved at the same polyphenol concentrations. These results demonstrate that GTPP can act as a biological antioxidant and protect veins from oxidative stress-induced toxicity.

Original languageEnglish
Pages (from-to)1137-1142
Number of pages6
JournalArtificial Organs
Volume27
Issue number12
DOIs
Publication statusPublished - 2003 Dec 1

Fingerprint

Oxidative stress
Saphenous Vein
Polyphenols
Tea
Veins
Reactive Oxygen Species
Oxidative Stress
Oxygen
Xanthine Oxidase
Histology
Xanthine
Endothelial cells
Antioxidants
Toxicity
Tissue
Endothelial Cells
Oxidoreductases

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Han, Dong Wook ; Suh, Hwal ; Park, Young Hwan ; Cho, Bum Koo ; Hyon, Suong Hyu ; Park, Jongchul. / Preservation of Human Saphenous Vein against Reactive Oxygen Species-induced Oxidative Stress by Green Tea Polyphenol Pretreatment. In: Artificial Organs. 2003 ; Vol. 27, No. 12. pp. 1137-1142.
@article{d582ed351771433fb00c803d4de3ba8f,
title = "Preservation of Human Saphenous Vein against Reactive Oxygen Species-induced Oxidative Stress by Green Tea Polyphenol Pretreatment",
abstract = "The injurious effects of reactive oxygen species on venous tissues and the potential protective role played by green tea polyphenol (GTPP) on human saphenous veins were investigated. Oxidative stress was induced exogenously in the vein segments, either by adding 0.8 or 1.6 M of H2O2, or by using 80 or 160 U/L of xanthine oxidase in the presence of xanthine (0.5 mM). After incubation, the viability of the endothelial cells dissociated from veins and the histology of the veins were evaluated. Due to both types of treatment, a significant decrease in cellular viability, severe morphological changes in the veins, and extracellular structural damage were induced. The H2O2-induced alterations were prevented by preincubating the veins with either 0.5 or 1.0 mg/ml of GTPP for 1 h. When the oxidative stress was induced by xanthine oxidase, cellular viability and venous structure were preserved at the same polyphenol concentrations. These results demonstrate that GTPP can act as a biological antioxidant and protect veins from oxidative stress-induced toxicity.",
author = "Han, {Dong Wook} and Hwal Suh and Park, {Young Hwan} and Cho, {Bum Koo} and Hyon, {Suong Hyu} and Jongchul Park",
year = "2003",
month = "12",
day = "1",
doi = "10.1111/j.1525-1594.2003.07163.x",
language = "English",
volume = "27",
pages = "1137--1142",
journal = "Artificial Organs",
issn = "0160-564X",
publisher = "Wiley-Blackwell",
number = "12",

}

Preservation of Human Saphenous Vein against Reactive Oxygen Species-induced Oxidative Stress by Green Tea Polyphenol Pretreatment. / Han, Dong Wook; Suh, Hwal; Park, Young Hwan; Cho, Bum Koo; Hyon, Suong Hyu; Park, Jongchul.

In: Artificial Organs, Vol. 27, No. 12, 01.12.2003, p. 1137-1142.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Preservation of Human Saphenous Vein against Reactive Oxygen Species-induced Oxidative Stress by Green Tea Polyphenol Pretreatment

AU - Han, Dong Wook

AU - Suh, Hwal

AU - Park, Young Hwan

AU - Cho, Bum Koo

AU - Hyon, Suong Hyu

AU - Park, Jongchul

PY - 2003/12/1

Y1 - 2003/12/1

N2 - The injurious effects of reactive oxygen species on venous tissues and the potential protective role played by green tea polyphenol (GTPP) on human saphenous veins were investigated. Oxidative stress was induced exogenously in the vein segments, either by adding 0.8 or 1.6 M of H2O2, or by using 80 or 160 U/L of xanthine oxidase in the presence of xanthine (0.5 mM). After incubation, the viability of the endothelial cells dissociated from veins and the histology of the veins were evaluated. Due to both types of treatment, a significant decrease in cellular viability, severe morphological changes in the veins, and extracellular structural damage were induced. The H2O2-induced alterations were prevented by preincubating the veins with either 0.5 or 1.0 mg/ml of GTPP for 1 h. When the oxidative stress was induced by xanthine oxidase, cellular viability and venous structure were preserved at the same polyphenol concentrations. These results demonstrate that GTPP can act as a biological antioxidant and protect veins from oxidative stress-induced toxicity.

AB - The injurious effects of reactive oxygen species on venous tissues and the potential protective role played by green tea polyphenol (GTPP) on human saphenous veins were investigated. Oxidative stress was induced exogenously in the vein segments, either by adding 0.8 or 1.6 M of H2O2, or by using 80 or 160 U/L of xanthine oxidase in the presence of xanthine (0.5 mM). After incubation, the viability of the endothelial cells dissociated from veins and the histology of the veins were evaluated. Due to both types of treatment, a significant decrease in cellular viability, severe morphological changes in the veins, and extracellular structural damage were induced. The H2O2-induced alterations were prevented by preincubating the veins with either 0.5 or 1.0 mg/ml of GTPP for 1 h. When the oxidative stress was induced by xanthine oxidase, cellular viability and venous structure were preserved at the same polyphenol concentrations. These results demonstrate that GTPP can act as a biological antioxidant and protect veins from oxidative stress-induced toxicity.

UR - http://www.scopus.com/inward/record.url?scp=0346599194&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346599194&partnerID=8YFLogxK

U2 - 10.1111/j.1525-1594.2003.07163.x

DO - 10.1111/j.1525-1594.2003.07163.x

M3 - Article

C2 - 14678430

AN - SCOPUS:0346599194

VL - 27

SP - 1137

EP - 1142

JO - Artificial Organs

JF - Artificial Organs

SN - 0160-564X

IS - 12

ER -