Objectives. To compare the rheological characteristics of structurally different hyaluronic acid (HA)-based biomaterials that are presently used for phonosurgery and to investigate their influence on the viscoelastic properties of vocal folds after implantation in an in vivo rabbit model. Study Design. In vitro and in vivo rheometric investigation. Setting. Experimental laboratory, Inha and Seoul National Universities. Methods. Viscoelastic shear properties of 3 HA-based biomaterials (Rofilan, Restylane, and Reviderm) were measured with a strain-controlled rheometer. These biomaterials were injected into the deep layers of rabbit vocal folds, and viscoelastic moduli of the injected vocal folds were determined 2 months after the injection. The vocal fold specimens were observed using a light microscope and a transmission electron microscope. Results. All HA-based biomaterials showed similar levels of shear viscosity, which were slightly higher than that of human vocal folds reported in previous studies. Compared with noninjected control vocal folds, there were no significant differences in the magnitudes of both elastic shear modulus (G') and viscous modulus (G″) of injected vocal folds among all of the materials. Light microscopic images showed that all materials were observed in the deep layers of vocal folds and electron scanning images revealed that injected HA particles were homogeneously distributed in regions of collagenous fibers. Conclusion. HA-based biomaterials could preserve the viscoelastic properties of the vocal folds, when they were injected into vocal folds in an in vivo rabbit model. However, further studies on the influence of the biomaterials on the viscoelasticity of human vocal folds in ECM surroundings are still needed.
|Number of pages||7|
|Journal||Otolaryngology - Head and Neck Surgery (United States)|
|Publication status||Published - 2012 Sep|
Bibliographical noteFunding Information:
Funding source: This study was supported by the Basic Science Research Program of the National Research Foundation (NRF) funded by the Korean Ministry of Education, Science and Technology (Grant 2010-0004316) and an Inha Research Grant. This study was also funded in part by the NIH National Institute on Deafness and Other Communication Disorders (NIDCD; Grant R01DC004224).
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