The release of reducing sugars (RS) upon various pretreatments and hydrolysis methods from de-oiled Jatropha waste (DJW) was studied. The highest RS concentration of 12.9g/L was observed at 10% enzyme hydrolysis. The next highest RS of 8.0g/L and 7.8g/L were obtained with 10% HCl and 2.5% H2SO4, respectively. The NaOH (2.5%), ultrasonication and heat (90°C for 60min) treatments showed the RS concentration of 2.5g/L, 1.1g/L and 2.0g/L, respectively. Autoclave treatment slightly enhanced the sugar release (0.9g/L) compared to no treatment (0.7g/L). Glucose release (11.4g/L) peaked in enzyme hydrolysis. Enzyme treated acid unhydrolysed biomass showed 11.1g/L RS. HCl and H2SO4 pretreatment gave maximal xylose (6.89g/L and 6.16g/L, respectively). Combined (acid and enzyme) hydrolysis employed was efficient and its subsequent batch hydrogen fermentation showed a production 3.1L H2/L reactor.
All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal