Prevalence and mechanisms of decreased susceptibility to carbapenems in Klebsiella pneumoniae isolates

Soo Young Kim, Yeon Joon Park, Jin Kyung Yu, Han Sik Kim, Yong Sung Park, Jong-Bok Yoon, Jin Young Yoo, Kyungwon Lee

Research output: Contribution to journalArticle

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Abstract

In this study, we examined the prevalence of and mechanisms of decreased susceptibility to either imipenem or meropenem in Klebsiella pneumoniae isolates. A total of 230 clinical isolates of K. pneumoniae were collected from 13 clinical laboratories from a nationwide distribution. The MICs of imipenem and meropenem were determined by the agar dilution method. To characterize the isolates with decreased susceptibility to carbapenems (MICs of >2 μg/mL), we performed polymerase chain reaction amplification of a variety of β-lactamase genes, isoelectric focusing, and outer membrane profile analysis using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry. Three isolates (BD6, BD8, and KN16) exhibited decreased susceptibility to carbapenems with imipenem MICs of 1, 4, and 8 μg/mL and meropenem MICs of 4, 8, and 4, respectively. Isolate BD6 produced bla TEM-1 , bla SHV-12 , and bla OXA-17 ; isolate BD8 produced bla GES-3 , bla SHV-12 , and bla OXA-17 ; and isolate KN16 produced bla TEM-11 , bla SHV-12 , and bla DHA-1 . In all the 3 isolates, OmpK35 porin was not expressed, and in 1 isolate (KN16), OmpK36 was not expressed either. The prevalence of decreased susceptibility to carbapenems was low (1.3%), and none of them showed overt resistance to carbapenems. Decreased susceptibility to carbapenems can occur in K. pneumoniae when bla GES-3 , bla TEM-11 , bla SHV-12 , bla OXA-17 , and/or bla DHA-1 are produced in combination with porin loss. In addition, to our knowledge, this is the 1st report of bla OXA-17 in Enterobacteriaceae.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Volume57
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

meropenem
Carbapenems
Klebsiella pneumoniae
Imipenem
Porins
Isoelectric Focusing
Enterobacteriaceae
Sodium Dodecyl Sulfate
Agar
Polyacrylamide Gel Electrophoresis
Mass Spectrometry
Cross-Sectional Studies
Polymerase Chain Reaction
Membranes
Asp(5)-oxytocin
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Kim, Soo Young ; Park, Yeon Joon ; Yu, Jin Kyung ; Kim, Han Sik ; Park, Yong Sung ; Yoon, Jong-Bok ; Yoo, Jin Young ; Lee, Kyungwon. / Prevalence and mechanisms of decreased susceptibility to carbapenems in Klebsiella pneumoniae isolates. In: Diagnostic Microbiology and Infectious Disease. 2007 ; Vol. 57, No. 1. pp. 85-91.
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abstract = "In this study, we examined the prevalence of and mechanisms of decreased susceptibility to either imipenem or meropenem in Klebsiella pneumoniae isolates. A total of 230 clinical isolates of K. pneumoniae were collected from 13 clinical laboratories from a nationwide distribution. The MICs of imipenem and meropenem were determined by the agar dilution method. To characterize the isolates with decreased susceptibility to carbapenems (MICs of >2 μg/mL), we performed polymerase chain reaction amplification of a variety of β-lactamase genes, isoelectric focusing, and outer membrane profile analysis using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry. Three isolates (BD6, BD8, and KN16) exhibited decreased susceptibility to carbapenems with imipenem MICs of 1, 4, and 8 μg/mL and meropenem MICs of 4, 8, and 4, respectively. Isolate BD6 produced bla TEM-1 , bla SHV-12 , and bla OXA-17 ; isolate BD8 produced bla GES-3 , bla SHV-12 , and bla OXA-17 ; and isolate KN16 produced bla TEM-11 , bla SHV-12 , and bla DHA-1 . In all the 3 isolates, OmpK35 porin was not expressed, and in 1 isolate (KN16), OmpK36 was not expressed either. The prevalence of decreased susceptibility to carbapenems was low (1.3{\%}), and none of them showed overt resistance to carbapenems. Decreased susceptibility to carbapenems can occur in K. pneumoniae when bla GES-3 , bla TEM-11 , bla SHV-12 , bla OXA-17 , and/or bla DHA-1 are produced in combination with porin loss. In addition, to our knowledge, this is the 1st report of bla OXA-17 in Enterobacteriaceae.",
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Prevalence and mechanisms of decreased susceptibility to carbapenems in Klebsiella pneumoniae isolates. / Kim, Soo Young; Park, Yeon Joon; Yu, Jin Kyung; Kim, Han Sik; Park, Yong Sung; Yoon, Jong-Bok; Yoo, Jin Young; Lee, Kyungwon.

In: Diagnostic Microbiology and Infectious Disease, Vol. 57, No. 1, 01.01.2007, p. 85-91.

Research output: Contribution to journalArticle

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AB - In this study, we examined the prevalence of and mechanisms of decreased susceptibility to either imipenem or meropenem in Klebsiella pneumoniae isolates. A total of 230 clinical isolates of K. pneumoniae were collected from 13 clinical laboratories from a nationwide distribution. The MICs of imipenem and meropenem were determined by the agar dilution method. To characterize the isolates with decreased susceptibility to carbapenems (MICs of >2 μg/mL), we performed polymerase chain reaction amplification of a variety of β-lactamase genes, isoelectric focusing, and outer membrane profile analysis using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry. Three isolates (BD6, BD8, and KN16) exhibited decreased susceptibility to carbapenems with imipenem MICs of 1, 4, and 8 μg/mL and meropenem MICs of 4, 8, and 4, respectively. Isolate BD6 produced bla TEM-1 , bla SHV-12 , and bla OXA-17 ; isolate BD8 produced bla GES-3 , bla SHV-12 , and bla OXA-17 ; and isolate KN16 produced bla TEM-11 , bla SHV-12 , and bla DHA-1 . In all the 3 isolates, OmpK35 porin was not expressed, and in 1 isolate (KN16), OmpK36 was not expressed either. The prevalence of decreased susceptibility to carbapenems was low (1.3%), and none of them showed overt resistance to carbapenems. Decreased susceptibility to carbapenems can occur in K. pneumoniae when bla GES-3 , bla TEM-11 , bla SHV-12 , bla OXA-17 , and/or bla DHA-1 are produced in combination with porin loss. In addition, to our knowledge, this is the 1st report of bla OXA-17 in Enterobacteriaceae.

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