Prevalence and molecular characteristics of carbapenemase-producing enterobacteriaceae from five hospitals in Korea

Seok Hoon Jeong, Han Sung Kim, Jae Seok Kim, Dong Hoon Shin, Hyun Soo Kim, Min Jeong Park, Saeam Shin, Jun Sung Hong, Seung Soon Lee, Wonkeun Song

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Abstract

Background: The emergence of carbapenemase-producing Enterobacteriaceae (CPE) represents a major clinical problem because these bacteria are resistant to most antibiotics. CPE remain relatively uncommon in Korea. We report the prevalence, clinical characteristics, and molecular epidemiology of CPE isolates collected from five university hospitals in Korea. Methods: Between January and December 2015, 393 non-duplicated isolates that were nonsusceptible to ertapenem were analyzed. Production of carbapenemase, extended-spectrum β-lactamase, and AmpC β-lactamase was determined by genotypic tests. Antimicrobial susceptibility profiles were determined by using an Etest. Clonality of Klebsiella pneumoniae carbapenemase (KPC)-2-producing and oxacillinase (OXA)-232-producing Klebsiella pneumoniae isolates was determined by pulsed-field gel electrophoresis (PFGE). Results: Of the 393 isolates tested, 79 (20.1%) were CPE. Of these 79 isolates, 47 (59.5%) harbored the blaOXA-232 gene while the remaining isolates carried genes blaKPC-2 (n=27), blaIMP-1(n=4), and blaNDM-1(n=1). Among the 24 KPC-2 K. pneumoniae isolates from hospital B, 100% were resistant to carbapenems, 8% to colistin, and 0% to tigecycline. Among the 45 OXA-232 K. pneumoniae at hospital C, 95% were resistant to ertapenem, 68% to imipenem, 95% to meropenem, 10% to colistin, and 24% to tigecycline. PFGE analysis revealed a unique pattern for KPC-2 K. pneumoniae and identified 30 isolates belonging to the dominant pulsotypes (PT)1 and PT2 among 41 OXA-232 K. pneumoniae isolates. Conclusions: CPE strains are present in Korea, with the majority of K. pneumoniae isolates producing OXA-232 and KPC-2. The prevalence and predominant genotypes of CPE show hospital-specific differences.

Original languageEnglish
Pages (from-to)529-535
Number of pages7
JournalAnnals of laboratory medicine
Volume36
Issue number6
DOIs
Publication statusPublished - 2016 Nov

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All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Jeong, S. H., Kim, H. S., Kim, J. S., Shin, D. H., Kim, H. S., Park, M. J., Shin, S., Hong, J. S., Lee, S. S., & Song, W. (2016). Prevalence and molecular characteristics of carbapenemase-producing enterobacteriaceae from five hospitals in Korea. Annals of laboratory medicine, 36(6), 529-535. https://doi.org/10.3343/alm.2016.36.6.529