Prevalence of acquired fosfomycin resistance among extended-spectrum β-lactamase-producing Escherichia coli and klebsiella pneumoniae clinical isolates in korea and IS26-composite transposon surrounding fosA3

So Young Lee, Yeon Joon Park, Jin Kyung Yu, Seungwon Jung, Yoonjoo Kim, Seokhoon Jeong, Yoshichika Arakawa

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Objectives: To investigate the prevalence of plasmid-mediated fosfomycin resistance determinants among extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae and their genetic environments. Methods: A total of 347 non-duplicate ESBL-producing E. coli (165) and K. pneumoniae (182) were collected. The fosfomycin MICs were determined by the agar dilution method according to CLSI guidelines. PCR was used to detect the plasmid-encoded fosfomycin resistance genes (fosA, fosA3, fosB and fosC2). For isolates harbouring plasmid-encoded fosfomycin resistance genes, sequence types (STs) were determined. The transformation experiment was performed using E. coli TOPO10 (Invitrogen, USA) as a recipient strain. With the plasmids from the transformants, plasmid replicon typing was performed and the nucleotide sequences adjacent to fosA3 were determined. Results: The susceptibility to fosfomycin was 92.9% in E. coli and 95.2% in K. pneumoniae. Of the 21 isolates non-susceptible to fosfomycin (8 E. coli and 13 K. pneumoniae), 7 (5 E. coli and 2 K. pneumoniae) isolates harboured fosA3 and all of them co-harboured blaCTX-M-1group or blaCTX-M-9group. The STs of the isolates harbouring fosA3 were diverse (E. coli: ST1, ST1, ST533, ST2 and ST86; K. pneumoniae: ST11 and ST101). The plasmid replicon types of transformants co-harbouring blaCTX-M-1group and blaCTX-M-9group were IncF and IncN, respectively. By sequence analysis, we found the common feature that the fosA3 gene, connected to blaCTX-M via insertion sequences, was located between two IS26 elements oriented in the opposite direction, composing an IS26-composite transposon. Conclusions: An IS26-composite transposon appears to be the main vehicle for dissemination of fosA3 in E. coli and K. pneumoniae of diverse clones.

Original languageEnglish
Article numberdks319
Pages (from-to)2843-2847
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Volume67
Issue number12
DOIs
Publication statusPublished - 2012 Dec 1

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Fosfomycin
Klebsiella pneumoniae
Korea
Escherichia coli
Plasmids
Replicon
Genes
Insertional Mutagenesis
Agar
Sequence Analysis
Clone Cells
Guidelines
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

@article{1210f855142d4ae6922485238f01e78f,
title = "Prevalence of acquired fosfomycin resistance among extended-spectrum β-lactamase-producing Escherichia coli and klebsiella pneumoniae clinical isolates in korea and IS26-composite transposon surrounding fosA3",
abstract = "Objectives: To investigate the prevalence of plasmid-mediated fosfomycin resistance determinants among extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae and their genetic environments. Methods: A total of 347 non-duplicate ESBL-producing E. coli (165) and K. pneumoniae (182) were collected. The fosfomycin MICs were determined by the agar dilution method according to CLSI guidelines. PCR was used to detect the plasmid-encoded fosfomycin resistance genes (fosA, fosA3, fosB and fosC2). For isolates harbouring plasmid-encoded fosfomycin resistance genes, sequence types (STs) were determined. The transformation experiment was performed using E. coli TOPO10 (Invitrogen, USA) as a recipient strain. With the plasmids from the transformants, plasmid replicon typing was performed and the nucleotide sequences adjacent to fosA3 were determined. Results: The susceptibility to fosfomycin was 92.9{\%} in E. coli and 95.2{\%} in K. pneumoniae. Of the 21 isolates non-susceptible to fosfomycin (8 E. coli and 13 K. pneumoniae), 7 (5 E. coli and 2 K. pneumoniae) isolates harboured fosA3 and all of them co-harboured blaCTX-M-1group or blaCTX-M-9group. The STs of the isolates harbouring fosA3 were diverse (E. coli: ST1, ST1, ST533, ST2 and ST86; K. pneumoniae: ST11 and ST101). The plasmid replicon types of transformants co-harbouring blaCTX-M-1group and blaCTX-M-9group were IncF and IncN, respectively. By sequence analysis, we found the common feature that the fosA3 gene, connected to blaCTX-M via insertion sequences, was located between two IS26 elements oriented in the opposite direction, composing an IS26-composite transposon. Conclusions: An IS26-composite transposon appears to be the main vehicle for dissemination of fosA3 in E. coli and K. pneumoniae of diverse clones.",
author = "Lee, {So Young} and Park, {Yeon Joon} and Yu, {Jin Kyung} and Seungwon Jung and Yoonjoo Kim and Seokhoon Jeong and Yoshichika Arakawa",
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language = "English",
volume = "67",
pages = "2843--2847",
journal = "Journal of Antimicrobial Chemotherapy",
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Prevalence of acquired fosfomycin resistance among extended-spectrum β-lactamase-producing Escherichia coli and klebsiella pneumoniae clinical isolates in korea and IS26-composite transposon surrounding fosA3. / Lee, So Young; Park, Yeon Joon; Yu, Jin Kyung; Jung, Seungwon; Kim, Yoonjoo; Jeong, Seokhoon; Arakawa, Yoshichika.

In: Journal of Antimicrobial Chemotherapy, Vol. 67, No. 12, dks319, 01.12.2012, p. 2843-2847.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Prevalence of acquired fosfomycin resistance among extended-spectrum β-lactamase-producing Escherichia coli and klebsiella pneumoniae clinical isolates in korea and IS26-composite transposon surrounding fosA3

AU - Lee, So Young

AU - Park, Yeon Joon

AU - Yu, Jin Kyung

AU - Jung, Seungwon

AU - Kim, Yoonjoo

AU - Jeong, Seokhoon

AU - Arakawa, Yoshichika

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Objectives: To investigate the prevalence of plasmid-mediated fosfomycin resistance determinants among extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae and their genetic environments. Methods: A total of 347 non-duplicate ESBL-producing E. coli (165) and K. pneumoniae (182) were collected. The fosfomycin MICs were determined by the agar dilution method according to CLSI guidelines. PCR was used to detect the plasmid-encoded fosfomycin resistance genes (fosA, fosA3, fosB and fosC2). For isolates harbouring plasmid-encoded fosfomycin resistance genes, sequence types (STs) were determined. The transformation experiment was performed using E. coli TOPO10 (Invitrogen, USA) as a recipient strain. With the plasmids from the transformants, plasmid replicon typing was performed and the nucleotide sequences adjacent to fosA3 were determined. Results: The susceptibility to fosfomycin was 92.9% in E. coli and 95.2% in K. pneumoniae. Of the 21 isolates non-susceptible to fosfomycin (8 E. coli and 13 K. pneumoniae), 7 (5 E. coli and 2 K. pneumoniae) isolates harboured fosA3 and all of them co-harboured blaCTX-M-1group or blaCTX-M-9group. The STs of the isolates harbouring fosA3 were diverse (E. coli: ST1, ST1, ST533, ST2 and ST86; K. pneumoniae: ST11 and ST101). The plasmid replicon types of transformants co-harbouring blaCTX-M-1group and blaCTX-M-9group were IncF and IncN, respectively. By sequence analysis, we found the common feature that the fosA3 gene, connected to blaCTX-M via insertion sequences, was located between two IS26 elements oriented in the opposite direction, composing an IS26-composite transposon. Conclusions: An IS26-composite transposon appears to be the main vehicle for dissemination of fosA3 in E. coli and K. pneumoniae of diverse clones.

AB - Objectives: To investigate the prevalence of plasmid-mediated fosfomycin resistance determinants among extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae and their genetic environments. Methods: A total of 347 non-duplicate ESBL-producing E. coli (165) and K. pneumoniae (182) were collected. The fosfomycin MICs were determined by the agar dilution method according to CLSI guidelines. PCR was used to detect the plasmid-encoded fosfomycin resistance genes (fosA, fosA3, fosB and fosC2). For isolates harbouring plasmid-encoded fosfomycin resistance genes, sequence types (STs) were determined. The transformation experiment was performed using E. coli TOPO10 (Invitrogen, USA) as a recipient strain. With the plasmids from the transformants, plasmid replicon typing was performed and the nucleotide sequences adjacent to fosA3 were determined. Results: The susceptibility to fosfomycin was 92.9% in E. coli and 95.2% in K. pneumoniae. Of the 21 isolates non-susceptible to fosfomycin (8 E. coli and 13 K. pneumoniae), 7 (5 E. coli and 2 K. pneumoniae) isolates harboured fosA3 and all of them co-harboured blaCTX-M-1group or blaCTX-M-9group. The STs of the isolates harbouring fosA3 were diverse (E. coli: ST1, ST1, ST533, ST2 and ST86; K. pneumoniae: ST11 and ST101). The plasmid replicon types of transformants co-harbouring blaCTX-M-1group and blaCTX-M-9group were IncF and IncN, respectively. By sequence analysis, we found the common feature that the fosA3 gene, connected to blaCTX-M via insertion sequences, was located between two IS26 elements oriented in the opposite direction, composing an IS26-composite transposon. Conclusions: An IS26-composite transposon appears to be the main vehicle for dissemination of fosA3 in E. coli and K. pneumoniae of diverse clones.

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U2 - 10.1093/jac/dks319

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JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

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M1 - dks319

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