Prevalence of plasmid-mediated AmpC β-lactamases in Escherichia coli and Klebsiella pneumoniae in Korea

Kyungwon Lee, Miae Lee, Jong Hee Shin, Myung Hee Lee, Sung Ha Kang, Ae Ja Park, DongEun Yong, Yunsop Chong

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae are relatively prevalent in Korea, suggesting dissemination of plasmid-mediated AmpC β-lactamases. In this study, 238 isolates of cefositin-resistant E. coli and K, pneumoniae (not including subspecies ozaenae and rhinoscleromatis) were collected in 2003 from 16 Korean hospitals. The prevalence of plasmid-mediated AmpC β-lactamases was determined by PCR. The AmpC gene alleles detected in E. coli and K. pneumoniae were blaDHA-1, 10 (8.6%) and 93 (76.2%); blaCMY-1-like, 14 (12.1%) and 2 (1.6%); and blaCMY-2-like, 38 (32.7%) and 1 (0.8%) isolates, respectively. The genes identified were blaDHA-1, blaCMY10-like, and blaCMY-2-like, and a new variant, blaCMY-18. Plasmid-mediated AmpC gene allele-positive isolates were present both in large city and in small province hospitals, as well as in isolates from outpatients. The proportions of plasmid-mediated AmpC gene-positive isolates were similar in both expanded spectrum β-lactamase (ESBL)-producing and -nonproducing isolates. In conclusion, DHA-1, CMY-2-like, and CMY-10-like plasmid-mediated AmpC β-lactamase-producing K. pneumoniae and E. coli isolates are widely disseminated in both large city and small province hospitals. Absence of blaCMY-1 and detection of a novel variant of blaCMY-2, blaCMY-18, indicate continued evolution of the prototype genes. Similar proportions of plasmid-mediated AmpC gene-positive isolates in both ESBL-producing and -nonproducing isolates suggest unhindered future spread of these resistances.

Original languageEnglish
Pages (from-to)44-49
Number of pages6
JournalMicrobial Drug Resistance
Volume12
Issue number1
DOIs
Publication statusPublished - 2006 Mar 1

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Klebsiella pneumoniae
Korea
Plasmids
Escherichia coli
Genes
Alleles
Cefoxitin
Pneumonia
Outpatients
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Pharmacology
  • Microbiology (medical)

Cite this

Lee, Kyungwon ; Lee, Miae ; Shin, Jong Hee ; Lee, Myung Hee ; Kang, Sung Ha ; Park, Ae Ja ; Yong, DongEun ; Chong, Yunsop. / Prevalence of plasmid-mediated AmpC β-lactamases in Escherichia coli and Klebsiella pneumoniae in Korea. In: Microbial Drug Resistance. 2006 ; Vol. 12, No. 1. pp. 44-49.
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abstract = "Cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae are relatively prevalent in Korea, suggesting dissemination of plasmid-mediated AmpC β-lactamases. In this study, 238 isolates of cefositin-resistant E. coli and K, pneumoniae (not including subspecies ozaenae and rhinoscleromatis) were collected in 2003 from 16 Korean hospitals. The prevalence of plasmid-mediated AmpC β-lactamases was determined by PCR. The AmpC gene alleles detected in E. coli and K. pneumoniae were blaDHA-1, 10 (8.6{\%}) and 93 (76.2{\%}); blaCMY-1-like, 14 (12.1{\%}) and 2 (1.6{\%}); and blaCMY-2-like, 38 (32.7{\%}) and 1 (0.8{\%}) isolates, respectively. The genes identified were blaDHA-1, blaCMY10-like, and blaCMY-2-like, and a new variant, blaCMY-18. Plasmid-mediated AmpC gene allele-positive isolates were present both in large city and in small province hospitals, as well as in isolates from outpatients. The proportions of plasmid-mediated AmpC gene-positive isolates were similar in both expanded spectrum β-lactamase (ESBL)-producing and -nonproducing isolates. In conclusion, DHA-1, CMY-2-like, and CMY-10-like plasmid-mediated AmpC β-lactamase-producing K. pneumoniae and E. coli isolates are widely disseminated in both large city and small province hospitals. Absence of blaCMY-1 and detection of a novel variant of blaCMY-2, blaCMY-18, indicate continued evolution of the prototype genes. Similar proportions of plasmid-mediated AmpC gene-positive isolates in both ESBL-producing and -nonproducing isolates suggest unhindered future spread of these resistances.",
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Prevalence of plasmid-mediated AmpC β-lactamases in Escherichia coli and Klebsiella pneumoniae in Korea. / Lee, Kyungwon; Lee, Miae; Shin, Jong Hee; Lee, Myung Hee; Kang, Sung Ha; Park, Ae Ja; Yong, DongEun; Chong, Yunsop.

In: Microbial Drug Resistance, Vol. 12, No. 1, 01.03.2006, p. 44-49.

Research output: Contribution to journalArticle

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AU - Lee, Kyungwon

AU - Lee, Miae

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N2 - Cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae are relatively prevalent in Korea, suggesting dissemination of plasmid-mediated AmpC β-lactamases. In this study, 238 isolates of cefositin-resistant E. coli and K, pneumoniae (not including subspecies ozaenae and rhinoscleromatis) were collected in 2003 from 16 Korean hospitals. The prevalence of plasmid-mediated AmpC β-lactamases was determined by PCR. The AmpC gene alleles detected in E. coli and K. pneumoniae were blaDHA-1, 10 (8.6%) and 93 (76.2%); blaCMY-1-like, 14 (12.1%) and 2 (1.6%); and blaCMY-2-like, 38 (32.7%) and 1 (0.8%) isolates, respectively. The genes identified were blaDHA-1, blaCMY10-like, and blaCMY-2-like, and a new variant, blaCMY-18. Plasmid-mediated AmpC gene allele-positive isolates were present both in large city and in small province hospitals, as well as in isolates from outpatients. The proportions of plasmid-mediated AmpC gene-positive isolates were similar in both expanded spectrum β-lactamase (ESBL)-producing and -nonproducing isolates. In conclusion, DHA-1, CMY-2-like, and CMY-10-like plasmid-mediated AmpC β-lactamase-producing K. pneumoniae and E. coli isolates are widely disseminated in both large city and small province hospitals. Absence of blaCMY-1 and detection of a novel variant of blaCMY-2, blaCMY-18, indicate continued evolution of the prototype genes. Similar proportions of plasmid-mediated AmpC gene-positive isolates in both ESBL-producing and -nonproducing isolates suggest unhindered future spread of these resistances.

AB - Cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae are relatively prevalent in Korea, suggesting dissemination of plasmid-mediated AmpC β-lactamases. In this study, 238 isolates of cefositin-resistant E. coli and K, pneumoniae (not including subspecies ozaenae and rhinoscleromatis) were collected in 2003 from 16 Korean hospitals. The prevalence of plasmid-mediated AmpC β-lactamases was determined by PCR. The AmpC gene alleles detected in E. coli and K. pneumoniae were blaDHA-1, 10 (8.6%) and 93 (76.2%); blaCMY-1-like, 14 (12.1%) and 2 (1.6%); and blaCMY-2-like, 38 (32.7%) and 1 (0.8%) isolates, respectively. The genes identified were blaDHA-1, blaCMY10-like, and blaCMY-2-like, and a new variant, blaCMY-18. Plasmid-mediated AmpC gene allele-positive isolates were present both in large city and in small province hospitals, as well as in isolates from outpatients. The proportions of plasmid-mediated AmpC gene-positive isolates were similar in both expanded spectrum β-lactamase (ESBL)-producing and -nonproducing isolates. In conclusion, DHA-1, CMY-2-like, and CMY-10-like plasmid-mediated AmpC β-lactamase-producing K. pneumoniae and E. coli isolates are widely disseminated in both large city and small province hospitals. Absence of blaCMY-1 and detection of a novel variant of blaCMY-2, blaCMY-18, indicate continued evolution of the prototype genes. Similar proportions of plasmid-mediated AmpC gene-positive isolates in both ESBL-producing and -nonproducing isolates suggest unhindered future spread of these resistances.

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