Prevalence of the VNIc genotype of Cryptococcus neoformans in non-HIV-associated cryptococcosis in the Republic of Korea

Young Hwa Choi, Popchai Ngamskulrungroj, Ashok Varma, Edward Sionov, Soo Myung Hwang, Fabian Carriconde, Wieland Meyer, Anastasia P. Litvintseva, Wee Gyo Lee, Jong Hee Shin, Eui Chong Kim, Kyungwon Lee, Tae Yeal Choi, Yeong Seon Lee, Kyung J. Kwon-Chung

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

PCR fingerprinting and multilocus sequence typing were applied to determine the major molecular types of the Cryptococcus neoformansCryptococcus gattii species complex in the Republic of Korea. Of the 78 strains isolated from patients diagnosed with cryptococcosis between 1990 and 2008, 96% were C. neoformans serotype A, mating type MATα and molecular type VNI. The remaining 4% were C. gattii, serotype B, mating type MATα and either molecular type VGIIb or VGIII. Of the 62 strains with known HIV status, only 14 (22.6%) were isolated from HIV-positive patients and belonged to molecular type VNI. Remarkably, 93% of the C. neoformans isolates had identical PCR fingerprint profiles with the VNIc genotype that has been identified recently as the major genotype among C. neoformans strains in China. Most strains (81.8%) of the VNIc genotype were associated with non-HIV patients compared with strains of the non-VNIc genotype (20%) (P=0.009). Unlike the Chinese strains, a majority (60%) of the non-HIV patients infected with strains of the VNIc genotype in the Republic of Korea had serious underlying conditions, with cancer and liver disease being the most common. This study affirms VNIc to be the most prevalent genotype of C. neoformans isolated from non-HIV patients with cryptococcosis.

Original languageEnglish
Pages (from-to)769-778
Number of pages10
JournalFEMS Yeast Research
Volume10
Issue number6
DOIs
Publication statusPublished - 2010 Sep 1

Fingerprint

Cryptococcosis
Republic of Korea
Cryptococcus neoformans
Genotype
Cryptococcus gattii
HIV
Multilocus Sequence Typing
Polymerase Chain Reaction
Dermatoglyphics
Liver Neoplasms
Liver Diseases
China

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Choi, Y. H., Ngamskulrungroj, P., Varma, A., Sionov, E., Hwang, S. M., Carriconde, F., ... Kwon-Chung, K. J. (2010). Prevalence of the VNIc genotype of Cryptococcus neoformans in non-HIV-associated cryptococcosis in the Republic of Korea. FEMS Yeast Research, 10(6), 769-778. https://doi.org/10.1111/j.1567-1364.2010.00648.x
Choi, Young Hwa ; Ngamskulrungroj, Popchai ; Varma, Ashok ; Sionov, Edward ; Hwang, Soo Myung ; Carriconde, Fabian ; Meyer, Wieland ; Litvintseva, Anastasia P. ; Lee, Wee Gyo ; Shin, Jong Hee ; Kim, Eui Chong ; Lee, Kyungwon ; Choi, Tae Yeal ; Lee, Yeong Seon ; Kwon-Chung, Kyung J. / Prevalence of the VNIc genotype of Cryptococcus neoformans in non-HIV-associated cryptococcosis in the Republic of Korea. In: FEMS Yeast Research. 2010 ; Vol. 10, No. 6. pp. 769-778.
@article{f2697bb67352471c9159c05d72af545a,
title = "Prevalence of the VNIc genotype of Cryptococcus neoformans in non-HIV-associated cryptococcosis in the Republic of Korea",
abstract = "PCR fingerprinting and multilocus sequence typing were applied to determine the major molecular types of the Cryptococcus neoformansCryptococcus gattii species complex in the Republic of Korea. Of the 78 strains isolated from patients diagnosed with cryptococcosis between 1990 and 2008, 96{\%} were C. neoformans serotype A, mating type MATα and molecular type VNI. The remaining 4{\%} were C. gattii, serotype B, mating type MATα and either molecular type VGIIb or VGIII. Of the 62 strains with known HIV status, only 14 (22.6{\%}) were isolated from HIV-positive patients and belonged to molecular type VNI. Remarkably, 93{\%} of the C. neoformans isolates had identical PCR fingerprint profiles with the VNIc genotype that has been identified recently as the major genotype among C. neoformans strains in China. Most strains (81.8{\%}) of the VNIc genotype were associated with non-HIV patients compared with strains of the non-VNIc genotype (20{\%}) (P=0.009). Unlike the Chinese strains, a majority (60{\%}) of the non-HIV patients infected with strains of the VNIc genotype in the Republic of Korea had serious underlying conditions, with cancer and liver disease being the most common. This study affirms VNIc to be the most prevalent genotype of C. neoformans isolated from non-HIV patients with cryptococcosis.",
author = "Choi, {Young Hwa} and Popchai Ngamskulrungroj and Ashok Varma and Edward Sionov and Hwang, {Soo Myung} and Fabian Carriconde and Wieland Meyer and Litvintseva, {Anastasia P.} and Lee, {Wee Gyo} and Shin, {Jong Hee} and Kim, {Eui Chong} and Kyungwon Lee and Choi, {Tae Yeal} and Lee, {Yeong Seon} and Kwon-Chung, {Kyung J.}",
year = "2010",
month = "9",
day = "1",
doi = "10.1111/j.1567-1364.2010.00648.x",
language = "English",
volume = "10",
pages = "769--778",
journal = "FEMS Yeast Research",
issn = "1567-1356",
publisher = "Wiley-Blackwell",
number = "6",

}

Choi, YH, Ngamskulrungroj, P, Varma, A, Sionov, E, Hwang, SM, Carriconde, F, Meyer, W, Litvintseva, AP, Lee, WG, Shin, JH, Kim, EC, Lee, K, Choi, TY, Lee, YS & Kwon-Chung, KJ 2010, 'Prevalence of the VNIc genotype of Cryptococcus neoformans in non-HIV-associated cryptococcosis in the Republic of Korea', FEMS Yeast Research, vol. 10, no. 6, pp. 769-778. https://doi.org/10.1111/j.1567-1364.2010.00648.x

Prevalence of the VNIc genotype of Cryptococcus neoformans in non-HIV-associated cryptococcosis in the Republic of Korea. / Choi, Young Hwa; Ngamskulrungroj, Popchai; Varma, Ashok; Sionov, Edward; Hwang, Soo Myung; Carriconde, Fabian; Meyer, Wieland; Litvintseva, Anastasia P.; Lee, Wee Gyo; Shin, Jong Hee; Kim, Eui Chong; Lee, Kyungwon; Choi, Tae Yeal; Lee, Yeong Seon; Kwon-Chung, Kyung J.

In: FEMS Yeast Research, Vol. 10, No. 6, 01.09.2010, p. 769-778.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Prevalence of the VNIc genotype of Cryptococcus neoformans in non-HIV-associated cryptococcosis in the Republic of Korea

AU - Choi, Young Hwa

AU - Ngamskulrungroj, Popchai

AU - Varma, Ashok

AU - Sionov, Edward

AU - Hwang, Soo Myung

AU - Carriconde, Fabian

AU - Meyer, Wieland

AU - Litvintseva, Anastasia P.

AU - Lee, Wee Gyo

AU - Shin, Jong Hee

AU - Kim, Eui Chong

AU - Lee, Kyungwon

AU - Choi, Tae Yeal

AU - Lee, Yeong Seon

AU - Kwon-Chung, Kyung J.

PY - 2010/9/1

Y1 - 2010/9/1

N2 - PCR fingerprinting and multilocus sequence typing were applied to determine the major molecular types of the Cryptococcus neoformansCryptococcus gattii species complex in the Republic of Korea. Of the 78 strains isolated from patients diagnosed with cryptococcosis between 1990 and 2008, 96% were C. neoformans serotype A, mating type MATα and molecular type VNI. The remaining 4% were C. gattii, serotype B, mating type MATα and either molecular type VGIIb or VGIII. Of the 62 strains with known HIV status, only 14 (22.6%) were isolated from HIV-positive patients and belonged to molecular type VNI. Remarkably, 93% of the C. neoformans isolates had identical PCR fingerprint profiles with the VNIc genotype that has been identified recently as the major genotype among C. neoformans strains in China. Most strains (81.8%) of the VNIc genotype were associated with non-HIV patients compared with strains of the non-VNIc genotype (20%) (P=0.009). Unlike the Chinese strains, a majority (60%) of the non-HIV patients infected with strains of the VNIc genotype in the Republic of Korea had serious underlying conditions, with cancer and liver disease being the most common. This study affirms VNIc to be the most prevalent genotype of C. neoformans isolated from non-HIV patients with cryptococcosis.

AB - PCR fingerprinting and multilocus sequence typing were applied to determine the major molecular types of the Cryptococcus neoformansCryptococcus gattii species complex in the Republic of Korea. Of the 78 strains isolated from patients diagnosed with cryptococcosis between 1990 and 2008, 96% were C. neoformans serotype A, mating type MATα and molecular type VNI. The remaining 4% were C. gattii, serotype B, mating type MATα and either molecular type VGIIb or VGIII. Of the 62 strains with known HIV status, only 14 (22.6%) were isolated from HIV-positive patients and belonged to molecular type VNI. Remarkably, 93% of the C. neoformans isolates had identical PCR fingerprint profiles with the VNIc genotype that has been identified recently as the major genotype among C. neoformans strains in China. Most strains (81.8%) of the VNIc genotype were associated with non-HIV patients compared with strains of the non-VNIc genotype (20%) (P=0.009). Unlike the Chinese strains, a majority (60%) of the non-HIV patients infected with strains of the VNIc genotype in the Republic of Korea had serious underlying conditions, with cancer and liver disease being the most common. This study affirms VNIc to be the most prevalent genotype of C. neoformans isolated from non-HIV patients with cryptococcosis.

UR - http://www.scopus.com/inward/record.url?scp=77955802922&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955802922&partnerID=8YFLogxK

U2 - 10.1111/j.1567-1364.2010.00648.x

DO - 10.1111/j.1567-1364.2010.00648.x

M3 - Article

VL - 10

SP - 769

EP - 778

JO - FEMS Yeast Research

JF - FEMS Yeast Research

SN - 1567-1356

IS - 6

ER -