Prevention of reactive oxygen species-induced oxidative stress in human microvascular endothelial cells by green tea polyphenol

Dong Kyun Rah, Dong Wook Han, Hyun Sook Baek, Suong Hyu Hyon, Jong Chul Park

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The potential protective roles played by green tea polyphenol (GTP) against the injurious effects of reactive oxygen species in human microvascular endothelial cells (HUMVECs) were investigated. Oxidative stress was induced in cultured HUMVECs, either by adding 10 mM H 2O 2 or by the action of 10 U/l xanthine oxidase (XO) in the presence of xanthine (250 μM). Both treatments produced a significant reduction (to 68% and 71%, respectively) in HUMVEC viability, as assessed by fluorescence double staining method followed by flow cytometric analysis. On the microscopic observations, the morphological changes and necrotic detachment were appreciably induced by both treatments. The H 2O 2-induced alterations were completely prevented by pre-incubating the ECs with 10 μg/ml GTP for 1 h. When the oxidative stress was induced by XO, the cell viability and morphology were also significantly maintained at the same GTP concentration. These results demonstrate that GTP can act as a biological antioxidant in a cell culture experimental model and prevent oxidative stress-induced cytotoxicity in ECs.

Original languageEnglish
Pages (from-to)269-275
Number of pages7
JournalToxicology Letters
Volume155
Issue number2
DOIs
Publication statusPublished - 2005 Feb 15

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Oxidative stress
Endothelial cells
Polyphenols
Tea
Reactive Oxygen Species
Oxidative Stress
Endothelial Cells
Xanthine Oxidase
Cell Survival
Xanthine
Cytotoxicity
Cell culture
Theoretical Models
Cell Culture Techniques
Antioxidants
Fluorescence
Cells
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Rah, Dong Kyun ; Han, Dong Wook ; Baek, Hyun Sook ; Hyon, Suong Hyu ; Park, Jong Chul. / Prevention of reactive oxygen species-induced oxidative stress in human microvascular endothelial cells by green tea polyphenol. In: Toxicology Letters. 2005 ; Vol. 155, No. 2. pp. 269-275.
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Prevention of reactive oxygen species-induced oxidative stress in human microvascular endothelial cells by green tea polyphenol. / Rah, Dong Kyun; Han, Dong Wook; Baek, Hyun Sook; Hyon, Suong Hyu; Park, Jong Chul.

In: Toxicology Letters, Vol. 155, No. 2, 15.02.2005, p. 269-275.

Research output: Contribution to journalArticle

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