Primary cilia-mediated osteogenic response to fluid flow occurs via increases in focal adhesion and akt signaling pathway in MC3T3-E1 osteoblastic cells

Ok Hee Jeon, Yeong Min Yoo, Kyung Hwan Kim, Christopher R. Jacobs, Chi Hyun Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fluid flow is an important anabolic mechanical signal that results in the upregulation of osteogenic responses in bone cells. Primary cilium may be responsible for the sensing of mechanical signals and converting these into biochemical signals to maintain bone homeostasis through an unknown mechanism. Focal adhesions, sites of mechanical linkage between the cytoskeleton and extracellular matrix, play a role as mechanoreceptors that are involved in fluid flow-induced signaling. In this study, we first verified whether focal adhesions are required for primary cilia to upregulate fluid flow-induced bone forming activities in osteoblasts. Specifically, mRNA and protein levels of cyclooxygenase- 2 (COX-2) and release of prostaglandin E2 (PGE2), which are important molecules that regulate bone formation, were quantified in MC3T3-E1 osteoblastic cells with or without primary cilia after exposure to 1 Pa of fluid flow-induced shear stress. Then, this experiment was continued on Arg-Gly-Asp-Ser (RGDS) peptide-treated cells after determining the formation of focal adhesions were inhibited. Second, signaling pathways, including Ras, ERK, and Akt, whereby primary cilia transmit fluid flow to cellular responses in osteoblastic cells were studied. Removal of primary cilia resulted in a significant decrease in COX-2 mRNA and protein and PGE2 release with a similar decrease in FAK mRNA and vinculin-positive focal adhesion sites in response to fluid flow. Furthermore, the removal of primary cilia suppressed the activation of p-Akt, but not Ras and p-ERK in response to fluid flow. Our results suggest that primary cilia lead to fluid flow-induced COX-2 induction and PGE2 release via increases in focal adhesions and their responses are activated by Akt phosphorylation in osteoblastic cells.

Original languageEnglish
Pages (from-to)379-388
Number of pages10
JournalCellular and Molecular Bioengineering
Volume4
Issue number3
DOIs
Publication statusPublished - 2011 Sep 1

Fingerprint

Focal Adhesions
Signaling Pathways
Cilia
Adhesion
Fluid Flow
Flow of fluids
Cell
Prostaglandins
Bone
arginyl-glycyl-aspartyl-serine
Cyclooxygenase 2
Dinoprostone
Messenger RNA
Bone and Bones
Up-Regulation
Vinculin
Mechanoreceptors
Proteins
Protein
Cytoskeleton

All Science Journal Classification (ASJC) codes

  • Modelling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

@article{069021516d834711a6670d6d328ee3a9,
title = "Primary cilia-mediated osteogenic response to fluid flow occurs via increases in focal adhesion and akt signaling pathway in MC3T3-E1 osteoblastic cells",
abstract = "Fluid flow is an important anabolic mechanical signal that results in the upregulation of osteogenic responses in bone cells. Primary cilium may be responsible for the sensing of mechanical signals and converting these into biochemical signals to maintain bone homeostasis through an unknown mechanism. Focal adhesions, sites of mechanical linkage between the cytoskeleton and extracellular matrix, play a role as mechanoreceptors that are involved in fluid flow-induced signaling. In this study, we first verified whether focal adhesions are required for primary cilia to upregulate fluid flow-induced bone forming activities in osteoblasts. Specifically, mRNA and protein levels of cyclooxygenase- 2 (COX-2) and release of prostaglandin E2 (PGE2), which are important molecules that regulate bone formation, were quantified in MC3T3-E1 osteoblastic cells with or without primary cilia after exposure to 1 Pa of fluid flow-induced shear stress. Then, this experiment was continued on Arg-Gly-Asp-Ser (RGDS) peptide-treated cells after determining the formation of focal adhesions were inhibited. Second, signaling pathways, including Ras, ERK, and Akt, whereby primary cilia transmit fluid flow to cellular responses in osteoblastic cells were studied. Removal of primary cilia resulted in a significant decrease in COX-2 mRNA and protein and PGE2 release with a similar decrease in FAK mRNA and vinculin-positive focal adhesion sites in response to fluid flow. Furthermore, the removal of primary cilia suppressed the activation of p-Akt, but not Ras and p-ERK in response to fluid flow. Our results suggest that primary cilia lead to fluid flow-induced COX-2 induction and PGE2 release via increases in focal adhesions and their responses are activated by Akt phosphorylation in osteoblastic cells.",
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Primary cilia-mediated osteogenic response to fluid flow occurs via increases in focal adhesion and akt signaling pathway in MC3T3-E1 osteoblastic cells. / Jeon, Ok Hee; Yoo, Yeong Min; Kim, Kyung Hwan; Jacobs, Christopher R.; Kim, Chi Hyun.

In: Cellular and Molecular Bioengineering, Vol. 4, No. 3, 01.09.2011, p. 379-388.

Research output: Contribution to journalArticle

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AU - Jeon, Ok Hee

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N2 - Fluid flow is an important anabolic mechanical signal that results in the upregulation of osteogenic responses in bone cells. Primary cilium may be responsible for the sensing of mechanical signals and converting these into biochemical signals to maintain bone homeostasis through an unknown mechanism. Focal adhesions, sites of mechanical linkage between the cytoskeleton and extracellular matrix, play a role as mechanoreceptors that are involved in fluid flow-induced signaling. In this study, we first verified whether focal adhesions are required for primary cilia to upregulate fluid flow-induced bone forming activities in osteoblasts. Specifically, mRNA and protein levels of cyclooxygenase- 2 (COX-2) and release of prostaglandin E2 (PGE2), which are important molecules that regulate bone formation, were quantified in MC3T3-E1 osteoblastic cells with or without primary cilia after exposure to 1 Pa of fluid flow-induced shear stress. Then, this experiment was continued on Arg-Gly-Asp-Ser (RGDS) peptide-treated cells after determining the formation of focal adhesions were inhibited. Second, signaling pathways, including Ras, ERK, and Akt, whereby primary cilia transmit fluid flow to cellular responses in osteoblastic cells were studied. Removal of primary cilia resulted in a significant decrease in COX-2 mRNA and protein and PGE2 release with a similar decrease in FAK mRNA and vinculin-positive focal adhesion sites in response to fluid flow. Furthermore, the removal of primary cilia suppressed the activation of p-Akt, but not Ras and p-ERK in response to fluid flow. Our results suggest that primary cilia lead to fluid flow-induced COX-2 induction and PGE2 release via increases in focal adhesions and their responses are activated by Akt phosphorylation in osteoblastic cells.

AB - Fluid flow is an important anabolic mechanical signal that results in the upregulation of osteogenic responses in bone cells. Primary cilium may be responsible for the sensing of mechanical signals and converting these into biochemical signals to maintain bone homeostasis through an unknown mechanism. Focal adhesions, sites of mechanical linkage between the cytoskeleton and extracellular matrix, play a role as mechanoreceptors that are involved in fluid flow-induced signaling. In this study, we first verified whether focal adhesions are required for primary cilia to upregulate fluid flow-induced bone forming activities in osteoblasts. Specifically, mRNA and protein levels of cyclooxygenase- 2 (COX-2) and release of prostaglandin E2 (PGE2), which are important molecules that regulate bone formation, were quantified in MC3T3-E1 osteoblastic cells with or without primary cilia after exposure to 1 Pa of fluid flow-induced shear stress. Then, this experiment was continued on Arg-Gly-Asp-Ser (RGDS) peptide-treated cells after determining the formation of focal adhesions were inhibited. Second, signaling pathways, including Ras, ERK, and Akt, whereby primary cilia transmit fluid flow to cellular responses in osteoblastic cells were studied. Removal of primary cilia resulted in a significant decrease in COX-2 mRNA and protein and PGE2 release with a similar decrease in FAK mRNA and vinculin-positive focal adhesion sites in response to fluid flow. Furthermore, the removal of primary cilia suppressed the activation of p-Akt, but not Ras and p-ERK in response to fluid flow. Our results suggest that primary cilia lead to fluid flow-induced COX-2 induction and PGE2 release via increases in focal adhesions and their responses are activated by Akt phosphorylation in osteoblastic cells.

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