Primary cilia negatively regulate melanogenesis in melanocytes and pigmentation in a human skin model

Hyunjung Choi, Ji Hyun Shin, Eun Sung Kim, So Jung Park, Il Hong Bae, Yoon Kyung Jo, In Young Jeong, Hyoung June Kim, Youngjin Lee, Hea Chul Park, Hong Bae Jeon, Kiwoo Kim, Tae Ryong Lee, Dong Hyung Cho

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The primary cilium is an organelle protruding from the cell body that senses external stimuli including chemical, mechanical, light, osmotic, fluid flow, and gravitational signals. Skin is always exposed to the external environment and responds to external stimuli. Therefore, it is possible that primary cilia have an important role in skin. Ciliogenesis was reported to be involved in developmental processes in skin, such as keratinocyte differentiation and hair formation. However, the relation between skin pigmentation and primary cilia is largely unknown. Here, we observed that increased melanogenesis in melanocytes treated with a melanogenic inducer was inhibited by a ciliogenesis inducer, cytochalasin D, and serumfree culture. However, these inhibitory effects disappeared in GLI2 knockdown cells. In addition, activation of sonic hedgehog (SHH)-smoothened (Smo) signaling pathway by a Smo agonist, SAG inhibited melanin synthesis in melanocytes and pigmentation in a human skin model. On the contrary, an inhibitor of primary cilium formation, ciliobrevin A1, activated melanogenesis in melanocytes. These results suggest that skin pigmentation may be regulated partly by the induction of ciliogenesis through Smo-GLI2 signaling.

Original languageEnglish
Article numbere0168025
JournalPloS one
Volume11
Issue number12
DOIs
Publication statusPublished - 2016 Dec 1

Fingerprint

melanogenesis
melanocytes
Cilia
Melanocytes
Pigmentation
cilia
skin (animal)
pigmentation
Skin
Skin Pigmentation
Cytochalasin D
Melanins
Keratinocytes
Organelles
Hair
cytochalasin D
Erinaceidae
keratinocytes
melanin
Light

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Choi, H., Shin, J. H., Kim, E. S., Park, S. J., Bae, I. H., Jo, Y. K., ... Cho, D. H. (2016). Primary cilia negatively regulate melanogenesis in melanocytes and pigmentation in a human skin model. PloS one, 11(12), [e0168025]. https://doi.org/10.1371/journal.pone.0168025
Choi, Hyunjung ; Shin, Ji Hyun ; Kim, Eun Sung ; Park, So Jung ; Bae, Il Hong ; Jo, Yoon Kyung ; Jeong, In Young ; Kim, Hyoung June ; Lee, Youngjin ; Park, Hea Chul ; Jeon, Hong Bae ; Kim, Kiwoo ; Lee, Tae Ryong ; Cho, Dong Hyung. / Primary cilia negatively regulate melanogenesis in melanocytes and pigmentation in a human skin model. In: PloS one. 2016 ; Vol. 11, No. 12.
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abstract = "The primary cilium is an organelle protruding from the cell body that senses external stimuli including chemical, mechanical, light, osmotic, fluid flow, and gravitational signals. Skin is always exposed to the external environment and responds to external stimuli. Therefore, it is possible that primary cilia have an important role in skin. Ciliogenesis was reported to be involved in developmental processes in skin, such as keratinocyte differentiation and hair formation. However, the relation between skin pigmentation and primary cilia is largely unknown. Here, we observed that increased melanogenesis in melanocytes treated with a melanogenic inducer was inhibited by a ciliogenesis inducer, cytochalasin D, and serumfree culture. However, these inhibitory effects disappeared in GLI2 knockdown cells. In addition, activation of sonic hedgehog (SHH)-smoothened (Smo) signaling pathway by a Smo agonist, SAG inhibited melanin synthesis in melanocytes and pigmentation in a human skin model. On the contrary, an inhibitor of primary cilium formation, ciliobrevin A1, activated melanogenesis in melanocytes. These results suggest that skin pigmentation may be regulated partly by the induction of ciliogenesis through Smo-GLI2 signaling.",
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Choi, H, Shin, JH, Kim, ES, Park, SJ, Bae, IH, Jo, YK, Jeong, IY, Kim, HJ, Lee, Y, Park, HC, Jeon, HB, Kim, K, Lee, TR & Cho, DH 2016, 'Primary cilia negatively regulate melanogenesis in melanocytes and pigmentation in a human skin model', PloS one, vol. 11, no. 12, e0168025. https://doi.org/10.1371/journal.pone.0168025

Primary cilia negatively regulate melanogenesis in melanocytes and pigmentation in a human skin model. / Choi, Hyunjung; Shin, Ji Hyun; Kim, Eun Sung; Park, So Jung; Bae, Il Hong; Jo, Yoon Kyung; Jeong, In Young; Kim, Hyoung June; Lee, Youngjin; Park, Hea Chul; Jeon, Hong Bae; Kim, Kiwoo; Lee, Tae Ryong; Cho, Dong Hyung.

In: PloS one, Vol. 11, No. 12, e0168025, 01.12.2016.

Research output: Contribution to journalArticle

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T1 - Primary cilia negatively regulate melanogenesis in melanocytes and pigmentation in a human skin model

AU - Choi, Hyunjung

AU - Shin, Ji Hyun

AU - Kim, Eun Sung

AU - Park, So Jung

AU - Bae, Il Hong

AU - Jo, Yoon Kyung

AU - Jeong, In Young

AU - Kim, Hyoung June

AU - Lee, Youngjin

AU - Park, Hea Chul

AU - Jeon, Hong Bae

AU - Kim, Kiwoo

AU - Lee, Tae Ryong

AU - Cho, Dong Hyung

PY - 2016/12/1

Y1 - 2016/12/1

N2 - The primary cilium is an organelle protruding from the cell body that senses external stimuli including chemical, mechanical, light, osmotic, fluid flow, and gravitational signals. Skin is always exposed to the external environment and responds to external stimuli. Therefore, it is possible that primary cilia have an important role in skin. Ciliogenesis was reported to be involved in developmental processes in skin, such as keratinocyte differentiation and hair formation. However, the relation between skin pigmentation and primary cilia is largely unknown. Here, we observed that increased melanogenesis in melanocytes treated with a melanogenic inducer was inhibited by a ciliogenesis inducer, cytochalasin D, and serumfree culture. However, these inhibitory effects disappeared in GLI2 knockdown cells. In addition, activation of sonic hedgehog (SHH)-smoothened (Smo) signaling pathway by a Smo agonist, SAG inhibited melanin synthesis in melanocytes and pigmentation in a human skin model. On the contrary, an inhibitor of primary cilium formation, ciliobrevin A1, activated melanogenesis in melanocytes. These results suggest that skin pigmentation may be regulated partly by the induction of ciliogenesis through Smo-GLI2 signaling.

AB - The primary cilium is an organelle protruding from the cell body that senses external stimuli including chemical, mechanical, light, osmotic, fluid flow, and gravitational signals. Skin is always exposed to the external environment and responds to external stimuli. Therefore, it is possible that primary cilia have an important role in skin. Ciliogenesis was reported to be involved in developmental processes in skin, such as keratinocyte differentiation and hair formation. However, the relation between skin pigmentation and primary cilia is largely unknown. Here, we observed that increased melanogenesis in melanocytes treated with a melanogenic inducer was inhibited by a ciliogenesis inducer, cytochalasin D, and serumfree culture. However, these inhibitory effects disappeared in GLI2 knockdown cells. In addition, activation of sonic hedgehog (SHH)-smoothened (Smo) signaling pathway by a Smo agonist, SAG inhibited melanin synthesis in melanocytes and pigmentation in a human skin model. On the contrary, an inhibitor of primary cilium formation, ciliobrevin A1, activated melanogenesis in melanocytes. These results suggest that skin pigmentation may be regulated partly by the induction of ciliogenesis through Smo-GLI2 signaling.

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