Prior airway exposure to allergen increases virus-induced airway hyperresponsiveness

Mika J. Mäkelä, Ralph Tripp, Azzeddine Dakhama, Jungwon Park, Toshihide Ikemura, Anthony Joetham, Matti Waris, Larry J. Anderson, Erwin W. Gelfand

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: Respiratory syncytial virus (RSV) bronchiolitis in early life can lead to changes in airway function, but there are likely additional predisposing factors, such as prior allergen exposure, determining which children develop wheezing and asthma. Objective: To define the effects of prior airway exposure to sensitizing allergen on the development of airway inflammation and hyperresponsiveness (AHR) to subsequent RSV infection. Methods: BALB/c mice were exposed to ovalbumin or PBS exclusively through the airways and subsequently infected with RSV or sham-inoculated. AHR, lung inflammation, and the frequency of cytokine-producing T lymphocytes in the lung were determined. Results: In PBS-exposed mice, RSV infection induced AHR and an increased proportion of TH1-type (IFN-γ and IL-12) cytokine-producing cells in the lungs. However, in mice previously exposed to ovalbumin through the airways and subsequently infected with RSV, the degree of AHR was significantly increased and was associated with an increased proportion of TH2 (IL-4, IL-5) cytokine-producing T lymphocytes. This response was also associated with an increased accumulation of eosinophils, neutrophils, and CDS+ T cells in the lungs. Conclusions: These data suggest that prior airway exposure to allergen may predispose sensitized hosts to a greater degree of altered airway function upon subsequent respiratory viral infection.

Original languageEnglish
Pages (from-to)861-869
Number of pages9
JournalJournal of Allergy and Clinical Immunology
Volume112
Issue number5
DOIs
Publication statusPublished - 2003 Jan 1

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Respiratory Syncytial Viruses
Allergens
Respiratory Syncytial Virus Infections
Ovalbumin
Cytokines
Viruses
T-Lymphocytes
Lung
Bronchiolitis
Interleukin-5
Respiratory Sounds
Virus Diseases
Interleukin-12
Eosinophils
Respiratory Tract Infections
Interleukin-4
Causality
Pneumonia
Neutrophils
Asthma

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Mäkelä, Mika J. ; Tripp, Ralph ; Dakhama, Azzeddine ; Park, Jungwon ; Ikemura, Toshihide ; Joetham, Anthony ; Waris, Matti ; Anderson, Larry J. ; Gelfand, Erwin W. / Prior airway exposure to allergen increases virus-induced airway hyperresponsiveness. In: Journal of Allergy and Clinical Immunology. 2003 ; Vol. 112, No. 5. pp. 861-869.
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Mäkelä, MJ, Tripp, R, Dakhama, A, Park, J, Ikemura, T, Joetham, A, Waris, M, Anderson, LJ & Gelfand, EW 2003, 'Prior airway exposure to allergen increases virus-induced airway hyperresponsiveness', Journal of Allergy and Clinical Immunology, vol. 112, no. 5, pp. 861-869. https://doi.org/10.1016/S0091-6749(03)02020-7

Prior airway exposure to allergen increases virus-induced airway hyperresponsiveness. / Mäkelä, Mika J.; Tripp, Ralph; Dakhama, Azzeddine; Park, Jungwon; Ikemura, Toshihide; Joetham, Anthony; Waris, Matti; Anderson, Larry J.; Gelfand, Erwin W.

In: Journal of Allergy and Clinical Immunology, Vol. 112, No. 5, 01.01.2003, p. 861-869.

Research output: Contribution to journalArticle

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AU - Mäkelä, Mika J.

AU - Tripp, Ralph

AU - Dakhama, Azzeddine

AU - Park, Jungwon

AU - Ikemura, Toshihide

AU - Joetham, Anthony

AU - Waris, Matti

AU - Anderson, Larry J.

AU - Gelfand, Erwin W.

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N2 - Background: Respiratory syncytial virus (RSV) bronchiolitis in early life can lead to changes in airway function, but there are likely additional predisposing factors, such as prior allergen exposure, determining which children develop wheezing and asthma. Objective: To define the effects of prior airway exposure to sensitizing allergen on the development of airway inflammation and hyperresponsiveness (AHR) to subsequent RSV infection. Methods: BALB/c mice were exposed to ovalbumin or PBS exclusively through the airways and subsequently infected with RSV or sham-inoculated. AHR, lung inflammation, and the frequency of cytokine-producing T lymphocytes in the lung were determined. Results: In PBS-exposed mice, RSV infection induced AHR and an increased proportion of TH1-type (IFN-γ and IL-12) cytokine-producing cells in the lungs. However, in mice previously exposed to ovalbumin through the airways and subsequently infected with RSV, the degree of AHR was significantly increased and was associated with an increased proportion of TH2 (IL-4, IL-5) cytokine-producing T lymphocytes. This response was also associated with an increased accumulation of eosinophils, neutrophils, and CDS+ T cells in the lungs. Conclusions: These data suggest that prior airway exposure to allergen may predispose sensitized hosts to a greater degree of altered airway function upon subsequent respiratory viral infection.

AB - Background: Respiratory syncytial virus (RSV) bronchiolitis in early life can lead to changes in airway function, but there are likely additional predisposing factors, such as prior allergen exposure, determining which children develop wheezing and asthma. Objective: To define the effects of prior airway exposure to sensitizing allergen on the development of airway inflammation and hyperresponsiveness (AHR) to subsequent RSV infection. Methods: BALB/c mice were exposed to ovalbumin or PBS exclusively through the airways and subsequently infected with RSV or sham-inoculated. AHR, lung inflammation, and the frequency of cytokine-producing T lymphocytes in the lung were determined. Results: In PBS-exposed mice, RSV infection induced AHR and an increased proportion of TH1-type (IFN-γ and IL-12) cytokine-producing cells in the lungs. However, in mice previously exposed to ovalbumin through the airways and subsequently infected with RSV, the degree of AHR was significantly increased and was associated with an increased proportion of TH2 (IL-4, IL-5) cytokine-producing T lymphocytes. This response was also associated with an increased accumulation of eosinophils, neutrophils, and CDS+ T cells in the lungs. Conclusions: These data suggest that prior airway exposure to allergen may predispose sensitized hosts to a greater degree of altered airway function upon subsequent respiratory viral infection.

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