Pro-apoptotic Noxa is involved in ablative focal irradiation-induced lung injury

Jee Youn Kim, Yong Min An, Won Hoon Choi, Jin Mo Kim, Samju Cho, Byung Rok Yoo, Jeong Wook Kang, Yun Sil Lee, Yoon Jin Lee, Jaeho Cho

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2 Citations (Scopus)

Abstract

Although lung injury including fibrosis is a well-documented side effect of lung irradiation, the mechanisms underlying its pathology are poorly understood. X-rays are known to cause apoptosis in the alveolar epithelial cells of irradiated lungs, which results in fibrosis due to the proliferation and differentiation of fibroblasts and the deposition of collagen. Apoptosis and BH3-only pro-apoptotic proteins have been implicated in the pathogenesis of pulmonary fibrosis. Recently, we have established a clinically analogous experimental model that reflects focal high-dose irradiation of the ipsilateral lung. The goal of this study was to elucidate the mechanism underlying radiation-induced lung injury based on this model. A radiation dose of 90 Gy was focally delivered to the left lung of C57BL/6 mice for 14 days. About 9 days after irradiation, the mice began to show increased levels of the pro-apoptotic protein Noxa in the irradiated lung alongside increased apoptosis and fibrosis. Suppression of Noxa expression by small interfering RNA protected cells from radiation-induced cell death and decreased expression of fibrogenic markers. Furthermore, we showed that reactive oxygen species participate in Noxa-mediated, radiation-induced cell death. Taken together, our results show that Noxa is involved in X-ray-induced lung injury.

Original languageEnglish
Pages (from-to)711-719
Number of pages9
JournalJournal of Cellular and Molecular Medicine
Volume21
Issue number4
DOIs
Publication statusPublished - 2017 Apr 1

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Cell Biology

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    Kim, J. Y., An, Y. M., Choi, W. H., Kim, J. M., Cho, S., Yoo, B. R., Kang, J. W., Lee, Y. S., Lee, Y. J., & Cho, J. (2017). Pro-apoptotic Noxa is involved in ablative focal irradiation-induced lung injury. Journal of Cellular and Molecular Medicine, 21(4), 711-719. https://doi.org/10.1111/jcmm.13014