Probing Cell-Surface Carbohydrate Binding Proteins with Dual-Modal Glycan-Conjugated Nanoparticles

Sungjin Park, Gun Hee Kim, Seong Hyun Park, Jaeyoung Pai, Dominea Rathwell, Jin Yeon Park, Young Sun Kang, Injae Shin

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Dual-modal fluorescent magnetic glyconanoparticles have been prepared and shown to be powerful in probing lectins displayed on pathogenic and mammalian cell surfaces. Blood group H1- and Leb-conjugated nanoparticles were found to bind to BabA displaying Helicobacter pylori, and Leª- and Leb-modified nanoparticles are both recognized by and internalized into DC-SIGN and SIGN-R1 expressing mammalian cells via lectin-mediated endocytosis. In addition, glyconanoparticles block adhesion of H. pylori to mammalian cells, suggesting that they can serve as inhibitors of infection of host cells by this pathogen. It has been also shown that owing to their magnetic properties, glyconanoparticles are useful tools to enrich lectin expressing cells. The combined results indicate that dual-modal glyconanoparticles are biocompatible and that they can be employed in lectin-associated biological studies and biomedical applications. (Figure Presented).

Original languageEnglish
Pages (from-to)5961-5968
Number of pages8
JournalJournal of the American Chemical Society
Volume137
Issue number18
DOIs
Publication statusPublished - 2015 May 13

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Probing Cell-Surface Carbohydrate Binding Proteins with Dual-Modal Glycan-Conjugated Nanoparticles'. Together they form a unique fingerprint.

Cite this