Abstract
Understanding how excitatory (E) and inhibitory (I) inputs are integrated by neurons requires monitoring their subthreshold behavior. We probed the subthreshold dynamics using optogenetic depolarizing pulses in hippocampal neuronal assemblies in freely moving mice. Excitability decreased during sharpwave ripples coupled with increased I. In contrast to this "negative gain," optogenetic probing showed increased within-field excitability in place cells by weakening I and unmasked stable place fields in initially non-place cells. Neuronal assemblies active during sharp-wave ripples in the home cage predicted spatial overlap and sequences of place fields of both place cells and unmasked preexisting place fields of non-place cells during track running. Thus, indirect probing of subthreshold dynamics in neuronal populations permits the disclosing of preexisting assemblies and modes of neuronal operations.
| Original language | English |
|---|---|
| Article number | 6580 |
| Journal | Science |
| Volume | 375 |
| Issue number | 6580 |
| DOIs | |
| Publication status | Published - 2022 Feb 4 |
Bibliographical note
Funding Information:1Neuroscience Institute, Langone Medical Center, New York University, New York, NY 10016, USA. 2Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA. 3Center for Nanomedicine, Institute for Basic Science (IBS) and Graduate Program of Nano Biomedical Engineering (Nano BME), Yonsei University, Seoul 03722, South Korea. 4Neuroscience Institute and Department of Neurology, Langone Medical Center, New York, NY 10016, USA. 5Center for Neural Science, New York University, New York, NY 10003, USA.
Publisher Copyright:
© 2022 American Association for the Advancement of Science. All rights reserved.
All Science Journal Classification (ASJC) codes
- General