Process optimisation for production and recovery of succinic acid using xylose-rich hydrolysates by Actinobacillus succinogenes

Esther Oreoluwa Jokodola; Vivek Narisetty; Eulogio Castro; Sumit Durgapal; Frederic Coulon; Raveendran Sindhu; Parameswaran Binod; J. Rajesh Banu; Gopalakrishnan Kumar; Vinod Kumar

doi:10.1016/j.biortech.2021.126224

Process optimisation for production and recovery of succinic acid using xylose-rich hydrolysates by Actinobacillus succinogenes

Esther Oreoluwa Jokodola, Vivek Narisetty, Eulogio Castro, Sumit Durgapal, Frederic Coulon, Raveendran Sindhu, Parameswaran Binod, J. Rajesh Banu, Gopalakrishnan Kumar, Vinod Kumar

Department of Civil and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Succinic acid (SA) is a top platform chemical obtainable from biomass. The current study evaluated the potential of Actinobacillus succinogenes for SA production using xylose-rich hemicellulosic fractions of two important lignocellulosic feedstocks, olive pits (OP) and sugarcane bagasse (SCB) and the results were compared with pure xylose. Initial experiments were conducted in shake flask followed by batch and fed-batch cultivation in bioreactor. Further separation of SA from the fermented broth was carried out by adapting direct crystallisation method. During fed-batch culture, maximum SA titers of 36.7, 33.6, and 28.7 g/L was achieved on pure xylose, OP and SCB hydrolysates, respectively, with same conversion yield of 0.27 g/g. The recovery yield of SA accumulated on pure xylose, OP and SCB hydrolysates was 79.1, 76.5, and 75.2%, respectively. The results obtained are of substantial value and pave the way for development of sustainable SA biomanufacturing in an integrated biorefinery.

Original language	English
Article number	126224
Journal	Bioresource technology
Volume	344
DOIs	https://doi.org/10.1016/j.biortech.2021.126224
Publication status	Published - 2022 Jan

Bibliographical note

Funding Information:
This study was financially supported through vWa Project (Grant BB/S011951/1) and we acknowledge BBSRC, Innovate UK and Department of Biotechnology, India for funding this project. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the article. We express gratitude to Cranfield University for providing facilities for conducting experiments. We acknowledge our industrial partner, Nova Pangaea Technologies for providing xylose rich SCB hydrolysate for this work.

Publisher Copyright:
© 2021 The Authors

All Science Journal Classification (ASJC) codes

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2021.126224

Cite this

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title = "Process optimisation for production and recovery of succinic acid using xylose-rich hydrolysates by Actinobacillus succinogenes",

abstract = "Succinic acid (SA) is a top platform chemical obtainable from biomass. The current study evaluated the potential of Actinobacillus succinogenes for SA production using xylose-rich hemicellulosic fractions of two important lignocellulosic feedstocks, olive pits (OP) and sugarcane bagasse (SCB) and the results were compared with pure xylose. Initial experiments were conducted in shake flask followed by batch and fed-batch cultivation in bioreactor. Further separation of SA from the fermented broth was carried out by adapting direct crystallisation method. During fed-batch culture, maximum SA titers of 36.7, 33.6, and 28.7 g/L was achieved on pure xylose, OP and SCB hydrolysates, respectively, with same conversion yield of 0.27 g/g. The recovery yield of SA accumulated on pure xylose, OP and SCB hydrolysates was 79.1, 76.5, and 75.2%, respectively. The results obtained are of substantial value and pave the way for development of sustainable SA biomanufacturing in an integrated biorefinery.",

author = "{Oreoluwa Jokodola}, Esther and Vivek Narisetty and Eulogio Castro and Sumit Durgapal and Frederic Coulon and Raveendran Sindhu and Parameswaran Binod and {Rajesh Banu}, J. and Gopalakrishnan Kumar and Vinod Kumar",

note = "Funding Information: This study was financially supported through vWa Project (Grant BB/S011951/1) and we acknowledge BBSRC, Innovate UK and Department of Biotechnology, India for funding this project. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the article. We express gratitude to Cranfield University for providing facilities for conducting experiments. We acknowledge our industrial partner, Nova Pangaea Technologies for providing xylose rich SCB hydrolysate for this work. Publisher Copyright: {\textcopyright} 2021 The Authors",

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language = "English",

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T1 - Process optimisation for production and recovery of succinic acid using xylose-rich hydrolysates by Actinobacillus succinogenes

AU - Oreoluwa Jokodola, Esther

AU - Narisetty, Vivek

AU - Castro, Eulogio

AU - Durgapal, Sumit

AU - Coulon, Frederic

AU - Sindhu, Raveendran

AU - Binod, Parameswaran

AU - Rajesh Banu, J.

AU - Kumar, Gopalakrishnan

AU - Kumar, Vinod

N1 - Funding Information: This study was financially supported through vWa Project (Grant BB/S011951/1) and we acknowledge BBSRC, Innovate UK and Department of Biotechnology, India for funding this project. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the article. We express gratitude to Cranfield University for providing facilities for conducting experiments. We acknowledge our industrial partner, Nova Pangaea Technologies for providing xylose rich SCB hydrolysate for this work. Publisher Copyright: © 2021 The Authors

PY - 2022/1

Y1 - 2022/1

N2 - Succinic acid (SA) is a top platform chemical obtainable from biomass. The current study evaluated the potential of Actinobacillus succinogenes for SA production using xylose-rich hemicellulosic fractions of two important lignocellulosic feedstocks, olive pits (OP) and sugarcane bagasse (SCB) and the results were compared with pure xylose. Initial experiments were conducted in shake flask followed by batch and fed-batch cultivation in bioreactor. Further separation of SA from the fermented broth was carried out by adapting direct crystallisation method. During fed-batch culture, maximum SA titers of 36.7, 33.6, and 28.7 g/L was achieved on pure xylose, OP and SCB hydrolysates, respectively, with same conversion yield of 0.27 g/g. The recovery yield of SA accumulated on pure xylose, OP and SCB hydrolysates was 79.1, 76.5, and 75.2%, respectively. The results obtained are of substantial value and pave the way for development of sustainable SA biomanufacturing in an integrated biorefinery.

AB - Succinic acid (SA) is a top platform chemical obtainable from biomass. The current study evaluated the potential of Actinobacillus succinogenes for SA production using xylose-rich hemicellulosic fractions of two important lignocellulosic feedstocks, olive pits (OP) and sugarcane bagasse (SCB) and the results were compared with pure xylose. Initial experiments were conducted in shake flask followed by batch and fed-batch cultivation in bioreactor. Further separation of SA from the fermented broth was carried out by adapting direct crystallisation method. During fed-batch culture, maximum SA titers of 36.7, 33.6, and 28.7 g/L was achieved on pure xylose, OP and SCB hydrolysates, respectively, with same conversion yield of 0.27 g/g. The recovery yield of SA accumulated on pure xylose, OP and SCB hydrolysates was 79.1, 76.5, and 75.2%, respectively. The results obtained are of substantial value and pave the way for development of sustainable SA biomanufacturing in an integrated biorefinery.

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Process optimisation for production and recovery of succinic acid using xylose-rich hydrolysates by Actinobacillus succinogenes

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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