Process optimisation for production and recovery of succinic acid using xylose-rich hydrolysates by Actinobacillus succinogenes

Esther Oreoluwa Jokodola, Vivek Narisetty, Eulogio Castro, Sumit Durgapal, Frederic Coulon, Raveendran Sindhu, Parameswaran Binod, J. Rajesh Banu, Gopalakrishnan Kumar, Vinod Kumar

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11 Citations (Scopus)


Succinic acid (SA) is a top platform chemical obtainable from biomass. The current study evaluated the potential of Actinobacillus succinogenes for SA production using xylose-rich hemicellulosic fractions of two important lignocellulosic feedstocks, olive pits (OP) and sugarcane bagasse (SCB) and the results were compared with pure xylose. Initial experiments were conducted in shake flask followed by batch and fed-batch cultivation in bioreactor. Further separation of SA from the fermented broth was carried out by adapting direct crystallisation method. During fed-batch culture, maximum SA titers of 36.7, 33.6, and 28.7 g/L was achieved on pure xylose, OP and SCB hydrolysates, respectively, with same conversion yield of 0.27 g/g. The recovery yield of SA accumulated on pure xylose, OP and SCB hydrolysates was 79.1, 76.5, and 75.2%, respectively. The results obtained are of substantial value and pave the way for development of sustainable SA biomanufacturing in an integrated biorefinery.

Original languageEnglish
Article number126224
JournalBioresource technology
Publication statusPublished - 2022 Jan

Bibliographical note

Funding Information:
This study was financially supported through vWa Project (Grant BB/S011951/1) and we acknowledge BBSRC, Innovate UK and Department of Biotechnology, India for funding this project. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the article. We express gratitude to Cranfield University for providing facilities for conducting experiments. We acknowledge our industrial partner, Nova Pangaea Technologies for providing xylose rich SCB hydrolysate for this work.

Publisher Copyright:
© 2021 The Authors

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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