Process performance of biohydrogen production using glucose at various HRTs and assessment of microbial dynamics variation via q-PCR

Arivalagan Pugazhendhi; Parthiban Anburajan; Jong Hun Park; Gopalakrishnan Kumar; Periyasamy Sivagurunathan; Sang Hyoun Kim

doi:10.1016/j.ijhydene.2017.06.184

Process performance of biohydrogen production using glucose at various HRTs and assessment of microbial dynamics variation via q-PCR

Arivalagan Pugazhendhi, Parthiban Anburajan, Jong Hun Park, Gopalakrishnan Kumar, Periyasamy Sivagurunathan, Sang Hyoun Kim

Department of Civil and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

This paper describes the continuous biohydrogen production in a mesophilic fixed-bed reactor using anaerobic digester sludge as an inoculum. Hydraulic retention times (HRTs) were decreased stepwise from 12 to 1.5 h, while 15 g/L of glucose was used as the model substrate. The peak hydrogen production performance was found at 1.5 h HRT with the hydrogen yield (HY) of 2.3 mol H₂/mol glucose_added and the hydrogen production rate (HPR) of 78 L H₂/L-d. Butyrate and acetate were the major soluble metabolic products released during the fermentation. Quantitative polymerase chain reaction (qPCR) and scanning electron microscopy analyses implied that Clostridium butyricum was dominant in the mixed culture fermentation in all the examined HRTs.

Original language	English
Pages (from-to)	27550-27557
Number of pages	8
Journal	International Journal of Hydrogen Energy
Volume	42
Issue number	45
DOIs	https://doi.org/10.1016/j.ijhydene.2017.06.184
Publication status	Published - 2017

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIP) (No. 2017R1A2A2A07000900).

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIP) (No. 2017R1A2A2A07000900 ).

Publisher Copyright:
© 2017 Hydrogen Energy Publications LLC

All Science Journal Classification (ASJC) codes

Renewable Energy, Sustainability and the Environment
Fuel Technology
Condensed Matter Physics
Energy Engineering and Power Technology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.ijhydene.2017.06.184

Cite this

@article{88edd6e5761a4716bb5c25489df6a42b,

title = "Process performance of biohydrogen production using glucose at various HRTs and assessment of microbial dynamics variation via q-PCR",

abstract = "This paper describes the continuous biohydrogen production in a mesophilic fixed-bed reactor using anaerobic digester sludge as an inoculum. Hydraulic retention times (HRTs) were decreased stepwise from 12 to 1.5 h, while 15 g/L of glucose was used as the model substrate. The peak hydrogen production performance was found at 1.5 h HRT with the hydrogen yield (HY) of 2.3 mol H2/mol glucoseadded and the hydrogen production rate (HPR) of 78 L H2/L-d. Butyrate and acetate were the major soluble metabolic products released during the fermentation. Quantitative polymerase chain reaction (qPCR) and scanning electron microscopy analyses implied that Clostridium butyricum was dominant in the mixed culture fermentation in all the examined HRTs.",

author = "Arivalagan Pugazhendhi and Parthiban Anburajan and Park, {Jong Hun} and Gopalakrishnan Kumar and Periyasamy Sivagurunathan and Kim, {Sang Hyoun}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIP) (No. 2017R1A2A2A07000900). Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIP) (No. 2017R1A2A2A07000900 ). Publisher Copyright: {\textcopyright} 2017 Hydrogen Energy Publications LLC",

year = "2017",

doi = "10.1016/j.ijhydene.2017.06.184",

language = "English",

volume = "42",

pages = "27550--27557",

journal = "International Journal of Hydrogen Energy",

issn = "0360-3199",

publisher = "Elsevier Limited",

number = "45",

}

TY - JOUR

T1 - Process performance of biohydrogen production using glucose at various HRTs and assessment of microbial dynamics variation via q-PCR

AU - Pugazhendhi, Arivalagan

AU - Anburajan, Parthiban

AU - Park, Jong Hun

AU - Kumar, Gopalakrishnan

AU - Sivagurunathan, Periyasamy

AU - Kim, Sang Hyoun

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIP) (No. 2017R1A2A2A07000900). Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIP) (No. 2017R1A2A2A07000900 ). Publisher Copyright: © 2017 Hydrogen Energy Publications LLC

PY - 2017

Y1 - 2017

N2 - This paper describes the continuous biohydrogen production in a mesophilic fixed-bed reactor using anaerobic digester sludge as an inoculum. Hydraulic retention times (HRTs) were decreased stepwise from 12 to 1.5 h, while 15 g/L of glucose was used as the model substrate. The peak hydrogen production performance was found at 1.5 h HRT with the hydrogen yield (HY) of 2.3 mol H2/mol glucoseadded and the hydrogen production rate (HPR) of 78 L H2/L-d. Butyrate and acetate were the major soluble metabolic products released during the fermentation. Quantitative polymerase chain reaction (qPCR) and scanning electron microscopy analyses implied that Clostridium butyricum was dominant in the mixed culture fermentation in all the examined HRTs.

AB - This paper describes the continuous biohydrogen production in a mesophilic fixed-bed reactor using anaerobic digester sludge as an inoculum. Hydraulic retention times (HRTs) were decreased stepwise from 12 to 1.5 h, while 15 g/L of glucose was used as the model substrate. The peak hydrogen production performance was found at 1.5 h HRT with the hydrogen yield (HY) of 2.3 mol H2/mol glucoseadded and the hydrogen production rate (HPR) of 78 L H2/L-d. Butyrate and acetate were the major soluble metabolic products released during the fermentation. Quantitative polymerase chain reaction (qPCR) and scanning electron microscopy analyses implied that Clostridium butyricum was dominant in the mixed culture fermentation in all the examined HRTs.

UR - http://www.scopus.com/inward/record.url?scp=85023188495&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85023188495&partnerID=8YFLogxK

U2 - 10.1016/j.ijhydene.2017.06.184

DO - 10.1016/j.ijhydene.2017.06.184

M3 - Article

AN - SCOPUS:85023188495

VL - 42

SP - 27550

EP - 27557

JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

SN - 0360-3199

IS - 45

ER -

Process performance of biohydrogen production using glucose at various HRTs and assessment of microbial dynamics variation via q-PCR

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this