Process performance of biohydrogen production using glucose at various HRTs and assessment of microbial dynamics variation via q-PCR

Arivalagan Pugazhendhi, Parthiban Anburajan, Jong Hun Park, Gopalakrishnan Kumar, Periyasamy Sivagurunathan, Sang-Hyoun Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This paper describes the continuous biohydrogen production in a mesophilic fixed-bed reactor using anaerobic digester sludge as an inoculum. Hydraulic retention times (HRTs) were decreased stepwise from 12 to 1.5 h, while 15 g/L of glucose was used as the model substrate. The peak hydrogen production performance was found at 1.5 h HRT with the hydrogen yield (HY) of 2.3 mol H2/mol glucoseadded and the hydrogen production rate (HPR) of 78 L H2/L-d. Butyrate and acetate were the major soluble metabolic products released during the fermentation. Quantitative polymerase chain reaction (qPCR) and scanning electron microscopy analyses implied that Clostridium butyricum was dominant in the mixed culture fermentation in all the examined HRTs.

Original languageEnglish
Pages (from-to)27550-27557
Number of pages8
JournalInternational Journal of Hydrogen Energy
Volume42
Issue number45
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

glucose
hydraulics
Glucose
fermentation
Hydraulics
hydrogen production
Hydrogen production
Fermentation
inoculum
Clostridium
polymerase chain reaction
sludge
Polymerase chain reaction
beds
acetates
reactors
Hydrogen
Scanning electron microscopy
scanning electron microscopy
Substrates

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

Pugazhendhi, Arivalagan ; Anburajan, Parthiban ; Park, Jong Hun ; Kumar, Gopalakrishnan ; Sivagurunathan, Periyasamy ; Kim, Sang-Hyoun. / Process performance of biohydrogen production using glucose at various HRTs and assessment of microbial dynamics variation via q-PCR. In: International Journal of Hydrogen Energy. 2017 ; Vol. 42, No. 45. pp. 27550-27557.
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Process performance of biohydrogen production using glucose at various HRTs and assessment of microbial dynamics variation via q-PCR. / Pugazhendhi, Arivalagan; Anburajan, Parthiban; Park, Jong Hun; Kumar, Gopalakrishnan; Sivagurunathan, Periyasamy; Kim, Sang-Hyoun.

In: International Journal of Hydrogen Energy, Vol. 42, No. 45, 01.01.2017, p. 27550-27557.

Research output: Contribution to journalArticle

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