Production and characterization of monoclonal antibodies to Treponema pallidum

Kyu Kwang Whang, Mingeol Lee, Wook Lew, Jung Bock Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study was attempted to produce the monoclonal antibodies specific for Treponema pallidum and to investigate their characteristics, thereby contributing to identify the antigenic structure and to apply the diagnosis of syphilis. The seven clones (YS 3, YS 75, YS 307, YS 481, YS 343, YS 1 and YS 406) secreting monoclonal antibodies reactive with T. pallidum were produced. The isotypes of the seven monoclonal antibodies produced were defined. Optical densities of the 7 monoclonal antibodies were ranged from 0.59 to 1.48 as measured by ELISA. Monclonal antibodies from 5 of the 7 clones which could agglutinate sheep RBC sensitized with T. pallidum, showed strong fluorescences. The monoclonal antibody from YS 343 was cross-reactive with non-pathogenic treponemes, but the other 6 monoclonal antibodies reacted with T. pallidum specifically. On immunoblotting, monoclonal antibodies from 5 of the 7 clones reacted with a polypeptide of a molecular weight of 47 kDa, and monoclonal antibodies from YS 343 reacted with a polypeptide of 64 kDa common to both T. pallidum and T. phagedenis. The above results revealed that 7 clones secreting monoclonal antibodies reactive to T. pallidum were produced successfully, and specific antibodies reacting with major antigenic polypeptides of a molecular weight of 47 kDa would be useful in the diagnosis of syphilis in the future.

Original languageEnglish
Pages (from-to)26-32
Number of pages7
JournalJournal of Dermatological Science
Volume4
Issue number1
DOIs
Publication statusPublished - 1992 Jan 1

Fingerprint

Treponema pallidum
Monoclonal Antibodies
Clone Cells
Syphilis
Peptides
Molecular Weight
Molecular weight
Density (optical)
Antibodies
Immunoblotting
Sheep
Fluorescence
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Whang, Kyu Kwang ; Lee, Mingeol ; Lew, Wook ; Lee, Jung Bock. / Production and characterization of monoclonal antibodies to Treponema pallidum. In: Journal of Dermatological Science. 1992 ; Vol. 4, No. 1. pp. 26-32.
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Production and characterization of monoclonal antibodies to Treponema pallidum. / Whang, Kyu Kwang; Lee, Mingeol; Lew, Wook; Lee, Jung Bock.

In: Journal of Dermatological Science, Vol. 4, No. 1, 01.01.1992, p. 26-32.

Research output: Contribution to journalArticle

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