Abstract
Phenolic glycolipid-I (PGL-I), a Mycobacterium leprae-specific antigen, has been widely used for the serodiagnosis of leprosy and has been implicated in the pathogenesis of leprosy. In an effort to produce an alternate antigen of PGL-I, the mimotope peptides of PGL-I, W(T/R)LGPY(V/M), were obtained using a monoclonal antibody, III603.8, specific to PGL-I by a phage library. The biotin-labeled predominant mimotope peptide of PGLP1, WTLGPYV, bound to III603.8 in a dose-dependent manner in an immunoassay. However, PGLP1 did not bind to anti-PGL-I antibodies in the serum samples from leprosy patients that were reactive to PGL-I. Although the mimotope peptide of WTLGPYV was not effective as an alternate antigen of PGL-I for the serodiagnosis of leprosy, but it would be of interest to know how the mimotope peptides mimic the role of PGL-I antigen in the pathogenesis of M. leprae infection.
| Original language | English |
|---|---|
| Pages (from-to) | 51-57 |
| Number of pages | 7 |
| Journal | FEMS Immunology and Medical Microbiology |
| Volume | 41 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2004 May 1 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Microbiology
- Immunology
- Microbiology (medical)
- Infectious Diseases
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Production and characterization of peptide mimotopes of phenolic glycolipid-I of Mycobacterium leprae. / Youn, Ju Ho; Myung, Han Jeong; Liav, Abraham; Chatterjee, Delphi; Brennan, Patrick J.; Choi, In Hong; Cho, Sangnae; Shin, Jeon Soo.
In: FEMS Immunology and Medical Microbiology, Vol. 41, No. 1, 01.05.2004, p. 51-57.Research output: Contribution to journal › Article
TY - JOUR
T1 - Production and characterization of peptide mimotopes of phenolic glycolipid-I of Mycobacterium leprae
AU - Youn, Ju Ho
AU - Myung, Han Jeong
AU - Liav, Abraham
AU - Chatterjee, Delphi
AU - Brennan, Patrick J.
AU - Choi, In Hong
AU - Cho, Sangnae
AU - Shin, Jeon Soo
PY - 2004/5/1
Y1 - 2004/5/1
N2 - Phenolic glycolipid-I (PGL-I), a Mycobacterium leprae-specific antigen, has been widely used for the serodiagnosis of leprosy and has been implicated in the pathogenesis of leprosy. In an effort to produce an alternate antigen of PGL-I, the mimotope peptides of PGL-I, W(T/R)LGPY(V/M), were obtained using a monoclonal antibody, III603.8, specific to PGL-I by a phage library. The biotin-labeled predominant mimotope peptide of PGLP1, WTLGPYV, bound to III603.8 in a dose-dependent manner in an immunoassay. However, PGLP1 did not bind to anti-PGL-I antibodies in the serum samples from leprosy patients that were reactive to PGL-I. Although the mimotope peptide of WTLGPYV was not effective as an alternate antigen of PGL-I for the serodiagnosis of leprosy, but it would be of interest to know how the mimotope peptides mimic the role of PGL-I antigen in the pathogenesis of M. leprae infection.
AB - Phenolic glycolipid-I (PGL-I), a Mycobacterium leprae-specific antigen, has been widely used for the serodiagnosis of leprosy and has been implicated in the pathogenesis of leprosy. In an effort to produce an alternate antigen of PGL-I, the mimotope peptides of PGL-I, W(T/R)LGPY(V/M), were obtained using a monoclonal antibody, III603.8, specific to PGL-I by a phage library. The biotin-labeled predominant mimotope peptide of PGLP1, WTLGPYV, bound to III603.8 in a dose-dependent manner in an immunoassay. However, PGLP1 did not bind to anti-PGL-I antibodies in the serum samples from leprosy patients that were reactive to PGL-I. Although the mimotope peptide of WTLGPYV was not effective as an alternate antigen of PGL-I for the serodiagnosis of leprosy, but it would be of interest to know how the mimotope peptides mimic the role of PGL-I antigen in the pathogenesis of M. leprae infection.
UR - http://www.scopus.com/inward/record.url?scp=16544392222&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=16544392222&partnerID=8YFLogxK
U2 - 10.1016/j.femsim.2004.01.001
DO - 10.1016/j.femsim.2004.01.001
M3 - Article
C2 - 15094167
AN - SCOPUS:16544392222
VL - 41
SP - 51
EP - 57
JO - Pathogens and Disease
JF - Pathogens and Disease
SN - 2049-632X
IS - 1
ER -