Production and characterization of peptide mimotopes of phenolic glycolipid-I of Mycobacterium leprae

Ju Ho Youn, Han Jeong Myung, Abraham Liav, Delphi Chatterjee, Patrick J. Brennan, In Hong Choi, Sangnae Cho, Jeon Soo Shin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Phenolic glycolipid-I (PGL-I), a Mycobacterium leprae-specific antigen, has been widely used for the serodiagnosis of leprosy and has been implicated in the pathogenesis of leprosy. In an effort to produce an alternate antigen of PGL-I, the mimotope peptides of PGL-I, W(T/R)LGPY(V/M), were obtained using a monoclonal antibody, III603.8, specific to PGL-I by a phage library. The biotin-labeled predominant mimotope peptide of PGLP1, WTLGPYV, bound to III603.8 in a dose-dependent manner in an immunoassay. However, PGLP1 did not bind to anti-PGL-I antibodies in the serum samples from leprosy patients that were reactive to PGL-I. Although the mimotope peptide of WTLGPYV was not effective as an alternate antigen of PGL-I for the serodiagnosis of leprosy, but it would be of interest to know how the mimotope peptides mimic the role of PGL-I antigen in the pathogenesis of M. leprae infection.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalFEMS Immunology and Medical Microbiology
Volume41
Issue number1
DOIs
Publication statusPublished - 2004 May 1

Fingerprint

Glycolipids
Leprosy
Peptides
Serologic Tests
Antigens
Mycobacterium leprae
Mycobacterium Infections
Biotin
Immunoassay
Bacteriophages
Mycobacterium leprae phenolic glycolipid I
Monoclonal Antibodies
Antibodies
Serum

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Youn, J. H., Myung, H. J., Liav, A., Chatterjee, D., Brennan, P. J., Choi, I. H., ... Shin, J. S. (2004). Production and characterization of peptide mimotopes of phenolic glycolipid-I of Mycobacterium leprae. FEMS Immunology and Medical Microbiology, 41(1), 51-57. https://doi.org/10.1016/j.femsim.2004.01.001
Youn, Ju Ho ; Myung, Han Jeong ; Liav, Abraham ; Chatterjee, Delphi ; Brennan, Patrick J. ; Choi, In Hong ; Cho, Sangnae ; Shin, Jeon Soo. / Production and characterization of peptide mimotopes of phenolic glycolipid-I of Mycobacterium leprae. In: FEMS Immunology and Medical Microbiology. 2004 ; Vol. 41, No. 1. pp. 51-57.
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Production and characterization of peptide mimotopes of phenolic glycolipid-I of Mycobacterium leprae. / Youn, Ju Ho; Myung, Han Jeong; Liav, Abraham; Chatterjee, Delphi; Brennan, Patrick J.; Choi, In Hong; Cho, Sangnae; Shin, Jeon Soo.

In: FEMS Immunology and Medical Microbiology, Vol. 41, No. 1, 01.05.2004, p. 51-57.

Research output: Contribution to journalArticle

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