Production and characterization of peptide mimotopes of phenolic glycolipid-I of Mycobacterium leprae

Ju Ho Youn, Han Jeong Myung, Abraham Liav, Delphi Chatterjee, Patrick J. Brennan, In Hong Choi, Sang Nae Cho, Jeon Soo Shin

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8 Citations (Scopus)

Abstract

Phenolic glycolipid-I (PGL-I), a Mycobacterium leprae-specific antigen, has been widely used for the serodiagnosis of leprosy and has been implicated in the pathogenesis of leprosy. In an effort to produce an alternate antigen of PGL-I, the mimotope peptides of PGL-I, W(T/R)LGPY(V/M), were obtained using a monoclonal antibody, III603.8, specific to PGL-I by a phage library. The biotin-labeled predominant mimotope peptide of PGLP1, WTLGPYV, bound to III603.8 in a dose-dependent manner in an immunoassay. However, PGLP1 did not bind to anti-PGL-I antibodies in the serum samples from leprosy patients that were reactive to PGL-I. Although the mimotope peptide of WTLGPYV was not effective as an alternate antigen of PGL-I for the serodiagnosis of leprosy, but it would be of interest to know how the mimotope peptides mimic the role of PGL-I antigen in the pathogenesis of M. leprae infection.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalFEMS Immunology and Medical Microbiology
Volume41
Issue number1
DOIs
Publication statusPublished - 2004 May 1

Bibliographical note

Funding Information:
The work was supported by a grant from The Heiser Program of The New York Community Trust, by a grant from the National Research Laboratory Program (M1-0204-00-0146), of the Ministry of Science and Technology National Research and Development Program, Korea, and by grant from National Institutes of Health (AI47197), USA. The PGL-I and its related antigens and neoglycoconjugates were provided via the contract NO1 AI55262 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, USA.

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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