Production and characterization of peptide mimotopes of phenolic glycolipid-I of Mycobacterium leprae

Ju Ho Youn, Han Jeong Myung, Abraham Liav, Delphi Chatterjee, Patrick J. Brennan, In Hong Choi, Sang Nae Cho, Jeon Soo Shin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Phenolic glycolipid-I (PGL-I), a Mycobacterium leprae-specific antigen, has been widely used for the serodiagnosis of leprosy and has been implicated in the pathogenesis of leprosy. In an effort to produce an alternate antigen of PGL-I, the mimotope peptides of PGL-I, W(T/R)LGPY(V/M), were obtained using a monoclonal antibody, III603.8, specific to PGL-I by a phage library. The biotin-labeled predominant mimotope peptide of PGLP1, WTLGPYV, bound to III603.8 in a dose-dependent manner in an immunoassay. However, PGLP1 did not bind to anti-PGL-I antibodies in the serum samples from leprosy patients that were reactive to PGL-I. Although the mimotope peptide of WTLGPYV was not effective as an alternate antigen of PGL-I for the serodiagnosis of leprosy, but it would be of interest to know how the mimotope peptides mimic the role of PGL-I antigen in the pathogenesis of M. leprae infection.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalFEMS Immunology and Medical Microbiology
Volume41
Issue number1
DOIs
Publication statusPublished - 2004 May 1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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