Production of γ-aminobutyric acid using immobilized glutamate decarboxylase from Lactobacillus plantarum

Sang Jae Lee, Han Seung Lee, Dong Woo Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The glutamate decarboxylase gene {gadB) from Lactobacillus plantarum WCFS1 was cloned and expressed as an N-terminal hexa-histidine-tagged fusion protein in Escherichia coli BL21 (DE3) as the host strain. Purified glutamate decarboxylase (GAD) was immobilized onto porous silica beads by covalent coupling. The pH dependence of activity and stability of the immobilized GAD was significantly altered, when compared to those of the free enzyme. Immobilized GAD was stable in the range of pH 3.5 to 6.0. The resulting packed-bed reactor produced 41.7 g of γ-aminobutyric acid/Ih at 45°C.

Original languageEnglish
Pages (from-to)300-305
Number of pages6
JournalKorean Journal of Microbiology and Biotechnology
Volume43
Issue number3
DOIs
Publication statusPublished - 2015 Sep 1

Fingerprint

Aminobutyrates
Lactobacillus plantarum
Glutamate Decarboxylase
Escherichia coli Proteins
Histidine
Silicon Dioxide
Enzymes
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "The glutamate decarboxylase gene {gadB) from Lactobacillus plantarum WCFS1 was cloned and expressed as an N-terminal hexa-histidine-tagged fusion protein in Escherichia coli BL21 (DE3) as the host strain. Purified glutamate decarboxylase (GAD) was immobilized onto porous silica beads by covalent coupling. The pH dependence of activity and stability of the immobilized GAD was significantly altered, when compared to those of the free enzyme. Immobilized GAD was stable in the range of pH 3.5 to 6.0. The resulting packed-bed reactor produced 41.7 g of γ-aminobutyric acid/Ih at 45°C.",
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Production of γ-aminobutyric acid using immobilized glutamate decarboxylase from Lactobacillus plantarum. / Lee, Sang Jae; Lee, Han Seung; Lee, Dong Woo.

In: Korean Journal of Microbiology and Biotechnology, Vol. 43, No. 3, 01.09.2015, p. 300-305.

Research output: Contribution to journalArticle

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