Production of (3-hydroxybutyrate-co-3-hydroxyhexanoate) copolymer from coffee waste oil using engineered Ralstonia eutropha

Shashi Kant Bhatia, Jung Ho Kim, Min Sun Kim, Junyoung Kim, Ju Won Hong, Yoon Gi Hong, Hyun Joong Kim, Jong Min Jeon, Sang-Hyoun Kim, Jungoh Ahn, Hongweon Lee, Yung Hun Yang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Polyhydroxyalkonate (PHA) is a type of polymer that has the potential to replace petro-based plastics. To make PHA production more economically feasible, there is a need to find a new carbon source and engineer microbes to produce a commercially valuable polymer. Coffee waste is an inexpensive raw material that contains fatty acids. It can act as a sustainable carbon source and seems quite promising with PHA production in Ralstonia eutropha, which is a well-known microbe for PHA accumulation, and has the potential to utilize fatty acids. In this study, to make poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)), which has superior properties in terms of biodegradability, biocompatibility, and mechanical strength, engineered strain Ralstonia eutropha Re2133 overexpressing (R)-specific enoyl coenzyme-A hydratase (phaJ) and PHA synthetase (phaC2) with deletion of acetoacetyl Co-A reductases (phaB1, phaB2, and phaB3) was used to produce PHA from coffee waste oil. At a coffee oil concentration of 1.5%, and C/N ratio of 20, the R. eutropha Re2133 fermentation process results in 69% w/w of DCW PHA accumulation and consists of HB (78 mol%) and HHx (22 mol%). This shows the feasibility of using coffee waste oil for P(HB-co-HHx) production, as it is a low-cost fatty acid enriched waste material.

Original languageEnglish
Pages (from-to)229-235
Number of pages7
JournalBioprocess and Biosystems Engineering
Volume41
Issue number2
DOIs
Publication statusPublished - 2018 Feb 1

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Cupriavidus necator
Coffee
3-Hydroxybutyric Acid
Oils
Copolymers
Fatty acids
Fatty Acids
Polymers
Carbon
Hydro-Lyases
Coenzymes
Biodegradability
Ligases
Biocompatibility
Fermentation
Plastics
Strength of materials
Raw materials
Oxidoreductases
Engineers

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering

Cite this

Bhatia, Shashi Kant ; Kim, Jung Ho ; Kim, Min Sun ; Kim, Junyoung ; Hong, Ju Won ; Hong, Yoon Gi ; Kim, Hyun Joong ; Jeon, Jong Min ; Kim, Sang-Hyoun ; Ahn, Jungoh ; Lee, Hongweon ; Yang, Yung Hun. / Production of (3-hydroxybutyrate-co-3-hydroxyhexanoate) copolymer from coffee waste oil using engineered Ralstonia eutropha. In: Bioprocess and Biosystems Engineering. 2018 ; Vol. 41, No. 2. pp. 229-235.
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abstract = "Polyhydroxyalkonate (PHA) is a type of polymer that has the potential to replace petro-based plastics. To make PHA production more economically feasible, there is a need to find a new carbon source and engineer microbes to produce a commercially valuable polymer. Coffee waste is an inexpensive raw material that contains fatty acids. It can act as a sustainable carbon source and seems quite promising with PHA production in Ralstonia eutropha, which is a well-known microbe for PHA accumulation, and has the potential to utilize fatty acids. In this study, to make poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)), which has superior properties in terms of biodegradability, biocompatibility, and mechanical strength, engineered strain Ralstonia eutropha Re2133 overexpressing (R)-specific enoyl coenzyme-A hydratase (phaJ) and PHA synthetase (phaC2) with deletion of acetoacetyl Co-A reductases (phaB1, phaB2, and phaB3) was used to produce PHA from coffee waste oil. At a coffee oil concentration of 1.5{\%}, and C/N ratio of 20, the R. eutropha Re2133 fermentation process results in 69{\%} w/w of DCW PHA accumulation and consists of HB (78 mol{\%}) and HHx (22 mol{\%}). This shows the feasibility of using coffee waste oil for P(HB-co-HHx) production, as it is a low-cost fatty acid enriched waste material.",
author = "Bhatia, {Shashi Kant} and Kim, {Jung Ho} and Kim, {Min Sun} and Junyoung Kim and Hong, {Ju Won} and Hong, {Yoon Gi} and Kim, {Hyun Joong} and Jeon, {Jong Min} and Sang-Hyoun Kim and Jungoh Ahn and Hongweon Lee and Yang, {Yung Hun}",
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Bhatia, SK, Kim, JH, Kim, MS, Kim, J, Hong, JW, Hong, YG, Kim, HJ, Jeon, JM, Kim, S-H, Ahn, J, Lee, H & Yang, YH 2018, 'Production of (3-hydroxybutyrate-co-3-hydroxyhexanoate) copolymer from coffee waste oil using engineered Ralstonia eutropha', Bioprocess and Biosystems Engineering, vol. 41, no. 2, pp. 229-235. https://doi.org/10.1007/s00449-017-1861-4

Production of (3-hydroxybutyrate-co-3-hydroxyhexanoate) copolymer from coffee waste oil using engineered Ralstonia eutropha. / Bhatia, Shashi Kant; Kim, Jung Ho; Kim, Min Sun; Kim, Junyoung; Hong, Ju Won; Hong, Yoon Gi; Kim, Hyun Joong; Jeon, Jong Min; Kim, Sang-Hyoun; Ahn, Jungoh; Lee, Hongweon; Yang, Yung Hun.

In: Bioprocess and Biosystems Engineering, Vol. 41, No. 2, 01.02.2018, p. 229-235.

Research output: Contribution to journalArticle

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T1 - Production of (3-hydroxybutyrate-co-3-hydroxyhexanoate) copolymer from coffee waste oil using engineered Ralstonia eutropha

AU - Bhatia, Shashi Kant

AU - Kim, Jung Ho

AU - Kim, Min Sun

AU - Kim, Junyoung

AU - Hong, Ju Won

AU - Hong, Yoon Gi

AU - Kim, Hyun Joong

AU - Jeon, Jong Min

AU - Kim, Sang-Hyoun

AU - Ahn, Jungoh

AU - Lee, Hongweon

AU - Yang, Yung Hun

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Polyhydroxyalkonate (PHA) is a type of polymer that has the potential to replace petro-based plastics. To make PHA production more economically feasible, there is a need to find a new carbon source and engineer microbes to produce a commercially valuable polymer. Coffee waste is an inexpensive raw material that contains fatty acids. It can act as a sustainable carbon source and seems quite promising with PHA production in Ralstonia eutropha, which is a well-known microbe for PHA accumulation, and has the potential to utilize fatty acids. In this study, to make poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)), which has superior properties in terms of biodegradability, biocompatibility, and mechanical strength, engineered strain Ralstonia eutropha Re2133 overexpressing (R)-specific enoyl coenzyme-A hydratase (phaJ) and PHA synthetase (phaC2) with deletion of acetoacetyl Co-A reductases (phaB1, phaB2, and phaB3) was used to produce PHA from coffee waste oil. At a coffee oil concentration of 1.5%, and C/N ratio of 20, the R. eutropha Re2133 fermentation process results in 69% w/w of DCW PHA accumulation and consists of HB (78 mol%) and HHx (22 mol%). This shows the feasibility of using coffee waste oil for P(HB-co-HHx) production, as it is a low-cost fatty acid enriched waste material.

AB - Polyhydroxyalkonate (PHA) is a type of polymer that has the potential to replace petro-based plastics. To make PHA production more economically feasible, there is a need to find a new carbon source and engineer microbes to produce a commercially valuable polymer. Coffee waste is an inexpensive raw material that contains fatty acids. It can act as a sustainable carbon source and seems quite promising with PHA production in Ralstonia eutropha, which is a well-known microbe for PHA accumulation, and has the potential to utilize fatty acids. In this study, to make poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)), which has superior properties in terms of biodegradability, biocompatibility, and mechanical strength, engineered strain Ralstonia eutropha Re2133 overexpressing (R)-specific enoyl coenzyme-A hydratase (phaJ) and PHA synthetase (phaC2) with deletion of acetoacetyl Co-A reductases (phaB1, phaB2, and phaB3) was used to produce PHA from coffee waste oil. At a coffee oil concentration of 1.5%, and C/N ratio of 20, the R. eutropha Re2133 fermentation process results in 69% w/w of DCW PHA accumulation and consists of HB (78 mol%) and HHx (22 mol%). This shows the feasibility of using coffee waste oil for P(HB-co-HHx) production, as it is a low-cost fatty acid enriched waste material.

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