Production of a new sucrose derivative by transglycosylation of recombinant Sulfolobus shibatae β-glycosidase

Na Young Park, Nam In Baek, Jaeho Cha, Soo-Bok Lee, Joong Hyuck Auh, Cheon Seok Park

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The gene encoding β-glycosidase of the hyperthermophilic archaea Sulfolobus shibatae (SSG) was expressed in Escherichia coli. Recombinant SSG (referred to as rSSG hereafter) was efficiently purified, and its transglycosylation activity was tested with lactose as a donor and various sugars as acceptors. When sucrose was used as an acceptor, we found a distinct intermolecular transglycosylation product and confirmed its presence by TLC and high performance anion exchange chromatography (HPAEC). The sucrose transglycosylation product was isolated by paper chromatography, and its chemical structure was determined by 1H and 13C NMR. The sucrose transfer product was determined to be β-d-galactopyranosyl- (1→6)-α-d-glucopyranosyl-β-d-fructofuranoside with a galactose molecule linked to sucrose via a β-(1→6)-glycosidic bond.

Original languageEnglish
Pages (from-to)1089-1096
Number of pages8
JournalCarbohydrate Research
Volume340
Issue number6
DOIs
Publication statusPublished - 2005 May 2

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Sulfolobus
Glycoside Hydrolases
Sucrose
Derivatives
Chromatography
Paper Chromatography
Gene encoding
Archaea
Lactose
Galactose
Sugars
Escherichia coli
Anions
Nuclear magnetic resonance
Molecules
Genes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Park, Na Young ; Baek, Nam In ; Cha, Jaeho ; Lee, Soo-Bok ; Auh, Joong Hyuck ; Park, Cheon Seok. / Production of a new sucrose derivative by transglycosylation of recombinant Sulfolobus shibatae β-glycosidase. In: Carbohydrate Research. 2005 ; Vol. 340, No. 6. pp. 1089-1096.
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Production of a new sucrose derivative by transglycosylation of recombinant Sulfolobus shibatae β-glycosidase. / Park, Na Young; Baek, Nam In; Cha, Jaeho; Lee, Soo-Bok; Auh, Joong Hyuck; Park, Cheon Seok.

In: Carbohydrate Research, Vol. 340, No. 6, 02.05.2005, p. 1089-1096.

Research output: Contribution to journalArticle

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