Production of a new sucrose derivative by transglycosylation of recombinant Sulfolobus shibatae β-glycosidase

Na Young Park, Nam In Baek, Jaeho Cha, Soo Bok Lee, Joong Hyuck Auh, Cheon Seok Park

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Abstract

The gene encoding β-glycosidase of the hyperthermophilic archaea Sulfolobus shibatae (SSG) was expressed in Escherichia coli. Recombinant SSG (referred to as rSSG hereafter) was efficiently purified, and its transglycosylation activity was tested with lactose as a donor and various sugars as acceptors. When sucrose was used as an acceptor, we found a distinct intermolecular transglycosylation product and confirmed its presence by TLC and high performance anion exchange chromatography (HPAEC). The sucrose transglycosylation product was isolated by paper chromatography, and its chemical structure was determined by 1H and 13C NMR. The sucrose transfer product was determined to be β-d-galactopyranosyl- (1→6)-α-d-glucopyranosyl-β-d-fructofuranoside with a galactose molecule linked to sucrose via a β-(1→6)-glycosidic bond.

Original languageEnglish
Pages (from-to)1089-1096
Number of pages8
JournalCarbohydrate Research
Volume340
Issue number6
DOIs
Publication statusPublished - 2005 May 2

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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