Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

Young Ho Hong, Dong Woo Lee, Sang Jae Lee, Eun Ah Choe, Seong Bo Kim, Yoon Hee Lee, Chan Ick Cheigh, Yu Ryang Pyun

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

Original languageEnglish
Pages (from-to)569-574
Number of pages6
JournalBiotechnology Letters
Volume29
Issue number4
DOIs
Publication statusPublished - 2007 Apr

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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