Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

Young Ho Hong, Dong Woo Lee, Sang Jae Lee, Eun Ah Choe, Seong Bo Kim, Yoon Hee Lee, Chan Ick Cheigh, Yu Ryang Pyun

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

Original languageEnglish
Pages (from-to)569-574
Number of pages6
JournalBiotechnology Letters
Volume29
Issue number4
DOIs
Publication statusPublished - 2007 Apr 1

Fingerprint

L-arabinose isomerase
Thermotoga neapolitana
Isomerases
Arabinose
Alginate
Galactose
Escherichia coli
Recycling
Calcium
Cells
Temperature
alginic acid
tagatose

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

Hong, Young Ho ; Lee, Dong Woo ; Lee, Sang Jae ; Choe, Eun Ah ; Kim, Seong Bo ; Lee, Yoon Hee ; Cheigh, Chan Ick ; Pyun, Yu Ryang. / Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana. In: Biotechnology Letters. 2007 ; Vol. 29, No. 4. pp. 569-574.
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Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana. / Hong, Young Ho; Lee, Dong Woo; Lee, Sang Jae; Choe, Eun Ah; Kim, Seong Bo; Lee, Yoon Hee; Cheigh, Chan Ick; Pyun, Yu Ryang.

In: Biotechnology Letters, Vol. 29, No. 4, 01.04.2007, p. 569-574.

Research output: Contribution to journalArticle

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AU - Kim, Seong Bo

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AU - Cheigh, Chan Ick

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