Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

Young Ho Hong, Dong Woo Lee, Sang Jae Lee, Eun Ah Choe, Seong Bo Kim, Yoon Hee Lee, Chan Ick Cheigh, Yu Ryang Pyun

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Abstract

Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

Original languageEnglish
Pages (from-to)569-574
Number of pages6
JournalBiotechnology Letters
Volume29
Issue number4
DOIs
Publication statusPublished - 2007 Apr 1

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All Science Journal Classification (ASJC) codes

  • Biotechnology

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