Production of oleamide, a functional lipid, by Streptomyces sp. KK90378

Ho Jeong Kwon, Sung Eun Hwang, Jae Taek Han, Chang Jin Kim, Jung Rae Rho, Jongheon Shin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Oleamide (cis-9-octadecenamide) is an endogenous primary amide of fatty acid that is produced in small amounts in animal brains. It is known to induce sleep and to lower temperature by destroying the lipid plasma membrane structure of cells, thereby disclosing gap junction channels. To develop a new biological production method for oleamide, a screening program was conducted to isolate a microorganism producing oleamide. Among 1,500 soil microorganisms tested, KK90378 exhibited a potent positive reaction with Dragendorff's reagent, used to detect the primary amide of oleamide. KK90378 was identified as a Streptomyces species based on cultural and morphological characteristics, the presence of diaminopimelic acid in the cell wall, and the sugar patterns for the whole-cell extract. Streptomyces sp. KK90378 produced oleamide 3 days after culture at 28°C, pH 7.2. A series of purification steps, including hexane extraction, silica gel column, and preparative thin layer chromatographies, were performed for the purification of oleamide. A spectrophotometric analysis using 1H, 13C-NMR, and GC-MS confirmed that the chemical structure of the purified oleamide was identical to that of authentic oleamide.

Original languageEnglish
Pages (from-to)1018-1023
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume11
Issue number6
Publication statusPublished - 2001 Dec 1

Fingerprint

Streptomyces
Amides
Microorganisms
Lipids
Purification
Thin layer chromatography
Membrane structures
Distillation columns
Silica gel
Cell membranes
Hexane
Fatty acids
Sugars
Brain
Screening
Animals
Cells
Nuclear magnetic resonance
Soils
Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Kwon, H. J., Hwang, S. E., Han, J. T., Kim, C. J., Rho, J. R., & Shin, J. (2001). Production of oleamide, a functional lipid, by Streptomyces sp. KK90378. Journal of Microbiology and Biotechnology, 11(6), 1018-1023.
Kwon, Ho Jeong ; Hwang, Sung Eun ; Han, Jae Taek ; Kim, Chang Jin ; Rho, Jung Rae ; Shin, Jongheon. / Production of oleamide, a functional lipid, by Streptomyces sp. KK90378. In: Journal of Microbiology and Biotechnology. 2001 ; Vol. 11, No. 6. pp. 1018-1023.
@article{f77eb4bb1e1f4366bf4b693f63c6d26d,
title = "Production of oleamide, a functional lipid, by Streptomyces sp. KK90378",
abstract = "Oleamide (cis-9-octadecenamide) is an endogenous primary amide of fatty acid that is produced in small amounts in animal brains. It is known to induce sleep and to lower temperature by destroying the lipid plasma membrane structure of cells, thereby disclosing gap junction channels. To develop a new biological production method for oleamide, a screening program was conducted to isolate a microorganism producing oleamide. Among 1,500 soil microorganisms tested, KK90378 exhibited a potent positive reaction with Dragendorff's reagent, used to detect the primary amide of oleamide. KK90378 was identified as a Streptomyces species based on cultural and morphological characteristics, the presence of diaminopimelic acid in the cell wall, and the sugar patterns for the whole-cell extract. Streptomyces sp. KK90378 produced oleamide 3 days after culture at 28°C, pH 7.2. A series of purification steps, including hexane extraction, silica gel column, and preparative thin layer chromatographies, were performed for the purification of oleamide. A spectrophotometric analysis using 1H, 13C-NMR, and GC-MS confirmed that the chemical structure of the purified oleamide was identical to that of authentic oleamide.",
author = "Kwon, {Ho Jeong} and Hwang, {Sung Eun} and Han, {Jae Taek} and Kim, {Chang Jin} and Rho, {Jung Rae} and Jongheon Shin",
year = "2001",
month = "12",
day = "1",
language = "English",
volume = "11",
pages = "1018--1023",
journal = "Journal of Microbiology and Biotechnology",
issn = "1017-7825",
publisher = "Korean Society for Microbiolog and Biotechnology",
number = "6",

}

Kwon, HJ, Hwang, SE, Han, JT, Kim, CJ, Rho, JR & Shin, J 2001, 'Production of oleamide, a functional lipid, by Streptomyces sp. KK90378', Journal of Microbiology and Biotechnology, vol. 11, no. 6, pp. 1018-1023.

Production of oleamide, a functional lipid, by Streptomyces sp. KK90378. / Kwon, Ho Jeong; Hwang, Sung Eun; Han, Jae Taek; Kim, Chang Jin; Rho, Jung Rae; Shin, Jongheon.

In: Journal of Microbiology and Biotechnology, Vol. 11, No. 6, 01.12.2001, p. 1018-1023.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Production of oleamide, a functional lipid, by Streptomyces sp. KK90378

AU - Kwon, Ho Jeong

AU - Hwang, Sung Eun

AU - Han, Jae Taek

AU - Kim, Chang Jin

AU - Rho, Jung Rae

AU - Shin, Jongheon

PY - 2001/12/1

Y1 - 2001/12/1

N2 - Oleamide (cis-9-octadecenamide) is an endogenous primary amide of fatty acid that is produced in small amounts in animal brains. It is known to induce sleep and to lower temperature by destroying the lipid plasma membrane structure of cells, thereby disclosing gap junction channels. To develop a new biological production method for oleamide, a screening program was conducted to isolate a microorganism producing oleamide. Among 1,500 soil microorganisms tested, KK90378 exhibited a potent positive reaction with Dragendorff's reagent, used to detect the primary amide of oleamide. KK90378 was identified as a Streptomyces species based on cultural and morphological characteristics, the presence of diaminopimelic acid in the cell wall, and the sugar patterns for the whole-cell extract. Streptomyces sp. KK90378 produced oleamide 3 days after culture at 28°C, pH 7.2. A series of purification steps, including hexane extraction, silica gel column, and preparative thin layer chromatographies, were performed for the purification of oleamide. A spectrophotometric analysis using 1H, 13C-NMR, and GC-MS confirmed that the chemical structure of the purified oleamide was identical to that of authentic oleamide.

AB - Oleamide (cis-9-octadecenamide) is an endogenous primary amide of fatty acid that is produced in small amounts in animal brains. It is known to induce sleep and to lower temperature by destroying the lipid plasma membrane structure of cells, thereby disclosing gap junction channels. To develop a new biological production method for oleamide, a screening program was conducted to isolate a microorganism producing oleamide. Among 1,500 soil microorganisms tested, KK90378 exhibited a potent positive reaction with Dragendorff's reagent, used to detect the primary amide of oleamide. KK90378 was identified as a Streptomyces species based on cultural and morphological characteristics, the presence of diaminopimelic acid in the cell wall, and the sugar patterns for the whole-cell extract. Streptomyces sp. KK90378 produced oleamide 3 days after culture at 28°C, pH 7.2. A series of purification steps, including hexane extraction, silica gel column, and preparative thin layer chromatographies, were performed for the purification of oleamide. A spectrophotometric analysis using 1H, 13C-NMR, and GC-MS confirmed that the chemical structure of the purified oleamide was identical to that of authentic oleamide.

UR - http://www.scopus.com/inward/record.url?scp=0035689898&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035689898&partnerID=8YFLogxK

M3 - Article

VL - 11

SP - 1018

EP - 1023

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

SN - 1017-7825

IS - 6

ER -