Production of transgenic bovine cloned embryos using piggybac transposition

Su Jin Kim, Islam M. Saadeldin, Woo Jae Choi, Song Jeon Lee, Won Wu Lee, Bong Han Kim, Hyo Jun Han, Duhee Bang, Byeong Chun Lee, Goo Jang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Transgenic research on cattle embryos has been developed to date using viral or plasmid DNA delivery systems. In this study, a different gene delivery system, piggybac transposition, was employed to investigate if it can be applied for producing transgenic cattle embryos. Green or red fluorescent proteins (GFP or RFP) were transfected into donor fibroblasts, and then transfected donor cells were reprogrammed in enucleated oocytes through SCNT and developed into pre-implantation stage embryos. GFP was expressed in donor cells and in cloned embryos without any mosaicism. Induction of RFP expression was regulated by doxycycline treatment in donor fibro-blasts and pre-implantational stage embryos. In conclusion, this study demonstrated that piggybac transposition could be a mean to deliver genes into bovine somatic cells or embryos for transgenic research.

Original languageEnglish
Pages (from-to)1453-1457
Number of pages5
JournalJournal of Veterinary Medical Science
Volume73
Issue number11
DOIs
Publication statusPublished - 2011 Jan 1

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transposition (genetics)
embryo (animal)
Embryonic Structures
genetically modified organisms
cattle
Embryo Research
Gene Transfer Techniques
Mosaicism
Doxycycline
Green Fluorescent Proteins
doxycycline
Oocytes
Plasmids
somatic embryos
somatic cells
gene transfer
Fibroblasts
fibroblasts
plasmids
oocytes

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Kim, S. J., Saadeldin, I. M., Choi, W. J., Lee, S. J., Lee, W. W., Kim, B. H., ... Jang, G. (2011). Production of transgenic bovine cloned embryos using piggybac transposition. Journal of Veterinary Medical Science, 73(11), 1453-1457. https://doi.org/10.1292/jvms.11-0054
Kim, Su Jin ; Saadeldin, Islam M. ; Choi, Woo Jae ; Lee, Song Jeon ; Lee, Won Wu ; Kim, Bong Han ; Han, Hyo Jun ; Bang, Duhee ; Lee, Byeong Chun ; Jang, Goo. / Production of transgenic bovine cloned embryos using piggybac transposition. In: Journal of Veterinary Medical Science. 2011 ; Vol. 73, No. 11. pp. 1453-1457.
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Kim, SJ, Saadeldin, IM, Choi, WJ, Lee, SJ, Lee, WW, Kim, BH, Han, HJ, Bang, D, Lee, BC & Jang, G 2011, 'Production of transgenic bovine cloned embryos using piggybac transposition', Journal of Veterinary Medical Science, vol. 73, no. 11, pp. 1453-1457. https://doi.org/10.1292/jvms.11-0054

Production of transgenic bovine cloned embryos using piggybac transposition. / Kim, Su Jin; Saadeldin, Islam M.; Choi, Woo Jae; Lee, Song Jeon; Lee, Won Wu; Kim, Bong Han; Han, Hyo Jun; Bang, Duhee; Lee, Byeong Chun; Jang, Goo.

In: Journal of Veterinary Medical Science, Vol. 73, No. 11, 01.01.2011, p. 1453-1457.

Research output: Contribution to journalArticle

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