Production of water-soluble pigment from mycelial culture of Cordyceps scarabaeicola KEFC-C252 and its antimutagenic effect

H. W. Lee, Hyung Sohn Joon Hyung Sohn, J. W. Choi, B. I. Yeh, W. S. Shin, J. B. Kim, H. W. Kim

Research output: Contribution to journalArticle

Abstract

Cultural conditions for the production of water-soluble pigment from mycelial culture of Cordyceps scarabaeicola KEFC-C252 and antimutagenic activity of the pigment were investigated. To obtain the maximum productivity of the pigment from mycelial culture of C. scarabaeicola KEFC-C252, the optimized medium was made with 1.5% sucrose, 2.5% yeast extract and initial pH 5.5. C. scarabaeicola KEFC-C252 was cultivated to reach the maximum concentration of the pigment at 26°C for 108 hrs. C. scarabaeicola KEFC-C252 produced about 1.2 g/liter pigment under the optimized condition. The pigment was isolated from the culture filtrate by ethylacetate extraction, acidic precipitation and crystallization. The isolated pigment was scarlet hexagonal column crystal, and the color of the pigment was changed according to pH of the solution. The pigment showed violet in the alkaline water but showed red color in the acidic water. The pigment showed inhibitory activity against mutagenic activity induced by 4-nitroquinoline N-oxide. Furthermore, the pigment showed inhibitory activity against spontaneous mutation on Salmonella typhimurium TA98 and TA100.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalKorean Journal of Applied Microbiology and Biotechnology
Volume28
Issue number2
Publication statusPublished - 2000 Jan 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology

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